Chemotaxis response regulator protein-glutamate methylesterase (P07330)

Identification
Name:Chemotaxis response regulator protein-glutamate methylesterase
Synonyms:Not Available
Gene Name:cheB
Enzyme Class:
Biological Properties
General Function:Involved in two-component response regulator activity
Specific Function:Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR
Cellular Location:Cytoplasm
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
1.0Protein glutamate methyl ester+1.0Thumb ↔ 1.0Protein glutamate+1.0Thumb
1.0Protein glutamate methyl ester + 1.0Water ↔ 1.0Protein glutamate + 1.0Methanol
ReactionCard
Complex Reactions:
1.0Protein L-glutamate O(5)-methyl ester+1.0Thumb → 1.0protein L-glutamate+1.0Thumb
1.0Protein L-glutamate O(5)-methyl ester + 1.0Water → 1.0protein L-glutamate + 1.0Methanol
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01875MethanolMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
carboxylesterase activity
catalytic activity
hydrolase activity
hydrolase activity, acting on ester bonds
molecular transducer activity
protein methylesterase activity
protein-glutamate methylesterase activity
signal transducer activity
two-component response regulator activity
Process
biological regulation
chemotaxis
regulation of biological process
regulation of gene expression
regulation of macromolecule metabolic process
regulation of metabolic process
regulation of transcription
regulation of transcription, DNA-dependent
response to chemical stimulus
response to stimulus
signal transmission
signaling process
two-component signal transduction system (phosphorelay)
Gene Properties
Blattner:b1883
Gene OrientationCounterclockwise
Centisome Percentage:42.36
Left Sequence End1965476
Right Sequence End1966525
Gene Sequence:
>1050 bp
ATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATC
ACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGC
CATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGACGGTTTACCCCTGCGTGCCG
GGTCATGAAATTGTGGGGCGTGTGGTAGCCGTTGGTGATCAGGTAGAAAAATATGCGCCG
GGCGATCTGGTCGGTGTCGGCTGCATTGTCGACAGTTGTAAACATTGCGAAGAGTGTGAA
GACGGGTTGGAAAACTACTGTGATCACATGACCGGCACCTATAACTCGCCGACGCCGGAC
GAACCGGGCCATACTCTGGGCGGCTACTCACAACAGATCGTCGTTCATGAGCGATATGTT
CTGCGTATTCGTCACCCGCAAGAGCAGCTGGCGGCGGTGGCTCCTTTGTTGTGTGCAGGG
ATCACCACGTATTCGCCGCTACGTCACTGGCAGGCCGGGCCGGGTAAAAAAGTGGGCGTG
GTCGGCATCGGCGGTCTGGGACATATGGGGATTAAGCTGGCCCACGCGATGGGGGCACAT
GTGGTGGCATTTACCACTTCTGAGGCAAAACGCGAAGCGGCAAAAGCCCTGGGGGCCGAT
GAAGTTGTTAACTCACGCAATGCCGATGAGATGGCGGCTCATCTGAAGAGTTTCGATTTC
ATTTTGAATACAGTAGCTGCGCCACATAATCTCGACGATTTTACCACCTTGCTGAAGCGT
GATGGCACCATGACGCTGGTTGGTGCGCCTGCGACACCGCATAAATCGCCGGAAGTTTTC
AACCTGATCATGAAACGCCGTGCGATAGCCGGTTCTATGATTGGCGGCATTCCAGAAACT
CAGGAGATGCTCGATTTTTGCGCCGAACATGGCATCGTGGCTGATATAGAGATGATTCGG
GCCGATCAAATTAATGAAGCCTATGAGCGAATGCTGCGCGGTGATGTGAAATATCGTTTT
GTTATCGATAATCGCACACTAACAGACTGA
Protein Properties
Pfam Domain Function:
Protein Residues:349
Protein Molecular Weight:37467
Protein Theoretical pI:9
PDB File:1A2O
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Chemotaxis response regulator protein-glutamate methylesterase
MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDLIKKFNPDVLTLDVEMP
RMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAIDFVTKPQLGIREGMLAYN
EMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIAIGASTGGTEAIRHVLQPLPL
SSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGERVLPGHAYIAPGDRHMELSRSG
ANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAVGVILTGMGNDGAAGMLAMRQAGAW
TLAQNEASCVVFGMPREAINMGGVCEVVDLSQVSQQMLAKISAGQAIRI
References
External Links:
ResourceLink
Uniprot ID:P07330
Uniprot Name:CHEB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674468
PDB ID:1A2O
Ecogene ID:EG10147
Ecocyc:EG10147
ColiBase:b1883
Kegg Gene:b1883
EchoBASE ID:EB0145
CCDB:CHEB_ECOLI
BacMap:16129835
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hess, J. F., Oosawa, K., Kaplan, N., Simon, M. I. (1988). "Phosphorylation of three proteins in the signaling pathway of bacterial chemotaxis." Cell 53:79-87. Pubmed: 3280143
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Mutoh, N., Simon, M. I. (1986). "Nucleotide sequence corresponding to five chemotaxis genes in Escherichia coli." J Bacteriol 165:161-166. Pubmed: 3510184