| Identification |
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| Name: | DNA topoisomerase 1 |
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| Synonyms: | Not Available |
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| Gene Name: | topA |
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| Enzyme Class: | |
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| Biological Properties |
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| General Function: | DNA topological change |
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| Specific Function: | Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. |
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| Cellular Location: | Not Available |
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| SMPDB Pathways: | Not Available |
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| KEGG Pathways: | Not Available |
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| Metabolites: | |
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| GO Classification: | | Function |
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| chromosome | | DNA binding | | DNA strand elongation | | DNA topoisomerase activity | | DNA topoisomerase type I activity | | DNA topoisomerase type II (ATP-hydrolyzing) activity | | DNA topological change | | magnesium ion binding |
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| Gene Properties |
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| Blattner: | Not Available |
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| Gene Orientation | Not Available |
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| Centisome Percentage: | Not Available |
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| Left Sequence End | Not Available |
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| Right Sequence End | Not Available |
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| Gene Sequence: | >2598
atgggtaaagctcttgtcatcgttgagtccccggcaaaagccaaaacgatcaacaagtat
ctgggtagtgactacgtggtgaaatccagcgtcggtcacatccgcgatttgccgaccagt
ggctcagctgccaaaaagagtgccgactctacctccaccaagacggctaaaaagcctaaa
aaggatgaacgtggcgctctcgtcaaccgtatgggggttgacccgtggcacaattgggag
gcgcactatgaagtgttgcctggtaaagagaaggtcgtctctgaactgaaacaactggct
gaaaaagccgaccacatctatctcgcaaccgaccttgaccgcgaaggggaagccattgca
tggcacctgcgggaagtgattgggggtgatgatgcgcgctatagccgagtggtgtttaac
gaaattactaaaaacgcgatccgccaggcatttaacaaaccgggtgagctgaatattgat
cgtgttaatgcccagcaggcgcgtcgctttatggaccgcgtggtggggtatatggtttcg
ccgctgctatggaaaaagatcgctcgtggcctgtctgccggtcgtgtgcagtcggtggcg
gttcgcctggtggtcgagcgtgagcgtgaaattaaagcgttcgtgccggaagagttctgg
gaagtcgatgccagcacgaccacgccatctggtgaagcgttggcgttacaggtgactcat
cagaacgacaaaccgttccgtccggtcaacaaagaacaaactcaggctgcggtaagtctg
ctggaaaaagcgcgctacagcgtgctggaacgtgaagacaaaccgacaaccagtaaacct
ggcgctccttttattacctctacgctgcaacaagctgccagcacccgtcttggatttggc
gtgaaaaaaaccatgatgatggcgcagcgtttgtatgaagcaggctatatcacttacatg
cgtaccgactccactaacctgagtcaggacgcggtaaatatggttcgcggttatatcagc
gataattttggtaagaaatatctgccggaaagtccgaatcagtacgccagcaaagaaaac
tcacaggaagcgcacgaagcgattcgtccttctgacgtcaatgtgatggcggaatcgctg
aaggatatggaagcagatgcgcagaaactgtaccagttaatctggcgtcagttcgttgcc
tgccagatgaccccagcgaaatatgactccacgacgctgaccgttggtgcgggcgatttc
cgcctgaaagcacgcggtcgtattttgcgttttgatggctggacaaaagtgatgcctgcg
ttgcgtaaaggcgatgaagatcgcatcttaccagcagttaataaaggcgatgctctgacg
ctcgttgaacttacaccagcccagcactttaccaagccgccagcccgtttcagtgaagca
tcgctggttaaagagctggaaaaacgcggtatcggtcgtccgtctacctatgcgtcgatc
atttcgaccattcaggatcgtggctacgtgcgagtagaaaatcgtcgtttctatgcggaa
aaaatgggcgaaatcgtcaccgatcgccttgaagaaaatttccgcgagttaatgaactac
gactttaccgcgcagatggaaaacagcctcgaccaggtggcaaatcacgaagcagagtgg
aaagctgtactggatcacttcttctcggatttcacccagcagttagataaagctgaaaaa
gatccggaagagggtggtatgcgcccgaaccagatggttctgaccagcattgactgcccg
acttgtggtcgcaaaatggggattcgcacagcgagcaccggggtattccttggctgttct
ggctatgcgctgccgccgaaagagcgttgcaaaaccaccattaacctggtgccggaaaac
gaagtgctgaacgtgctggaaggcgaagatgctgaaaccaacgcgctgcgcgcaaaacgt
cgttgcccgaaatgcggcacggcgatggacagctatctcatcgatccgaaacgtaagttg
catgtctgtggtaataacccaacctgcgacggttacgagatcgaagagggcgaattccgc
attaaaggttatgacggcccgatcgttgagtgtgaaaaatgtggctctgaaatgcacctg
aaaatggggcgtttcggtaaatacatggcctgcaccaacgaagagtgtaaaaacacacgt
aagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgag
ctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttc
ctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctt
tatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccacagcag
gatccggaaggtaataagaccatggttcgctttagccgtaaaaccaaacagcaatatgtc
tcttcggaaaaagacggaaaggcgactggctggtcagcattttatgttgatggcaaatgg
gttgaaggaaaaaaataa |
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| Protein Properties |
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| Pfam Domain Function: | Not Available |
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| Protein Residues: | 865 |
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| Protein Molecular Weight: | 97348 |
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| Protein Theoretical pI: | Not Available |
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| PDB File: | 1CY0 |
| Signaling Regions: | Not Available |
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| Transmembrane Regions: | Not Available |
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| Protein Sequence: | >DNA topoisomerase 1
MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAAKKSADSTSTKTAKKPK
KDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIA
WHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNIDRVNAQQARRFMDRVVGYMVS
PLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDASTTTPSGEALALQVTH
QNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRLGFG
VKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNMVRGYISDNFGKKYLPESPNQYASKEN
SQEAHEAIRPSDVNVMAESLKDMEADAQKLYQLIWRQFVACQMTPAKYDSTTLTVGAGDF
RLKARGRILRFDGWTKVMPALRKGDEDRILPAVNKGDALTLVELTPAQHFTKPPARFSEA
SLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNY
DFTAQMENSLDQVANHEAEWKAVLDHFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCP
TCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPENEVLNVLEGEDAETNALRAKR
RCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHL
KMGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVF
LAANTFPKSRETRAPLVEELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYV
SSEKDGKATGWSAFYVDGKWVEGKK |
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| References |
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| External Links: | |
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| General Reference: | Not Available |
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