Identification
Name:tRNA N6-adenosine threonylcarbamoyltransferase
Synonyms:Not Available
Gene Name:tsaD
Enzyme Class:
Biological Properties
General Function:threonylcarbamoyladenosine biosynthetic process
Specific Function:Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction. May also be involved in the metabolism of glycated proteins, but does not show sialoglycoprotease activity against glycophorin A.
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB00045Adenosine monophosphateMetaboCard
ECMDB23796L-ThreonylcarbamoyladenylateMetaboCard
GO Classification:
Function
cytosol
glycoprotein binding
identical protein binding
iron ion binding
metalloendopeptidase activity
peptidase activity
proteolysis
threonylcarbamoyladenosine biosynthetic process
transferase activity, transferring acyl groups other than amino-acyl groups
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>1014
atgcgtgtactgggtattgaaacttcctgcgatgaaaccggcatcgccatttacgacgat
gaaaaaggtttgttagccaaccaattgtatagtcaggtgaaattgcacgctgactacggc
ggcgtcgtgcctgaactggcctcccgcgatcatgtgcgtaaaaccgtaccgttgatccag
gcggcgctaaaggagtctggtttaacggcaaaagacattgatgctgtggcctataccgca
ggccctggattagtcggcgcgctactggttggcgcgaccgtggggcgttctctggcgttt
gcctgggacgttccggcgatccctgtacaccatatggaagggcatctgttagcgccgatg
ctggaagataacccgccggaatttccgtttgttgcgctgcttgtttccggcggtcatacg
cagttaatcagcgtgactggcattggtcagtacgagctgctcggcgagtctatcgatgat
gccgccggggaagcgtttgataaaaccgcgaagctgctggggctggattatcctggcggg
ccgttactgtcgaaaatggcggctcagggtactgccgggcgctttgtcttcccgcgtccg
atgaccgaccgtccggggctggatttcagcttctccggcctgaaaaccttcgcggcaaat
accattcgtgacaacggcaccgacgaccagacgcgtgctgatatcgcccgcgcctttgaa
gatgcggtggtcgatacgctgatgattaagtgcaagcgggcgctggatcagacgggcttt
aagcgactggtcatggcgggcggcgtgagtgctaaccgtacgttacgggcgaagctggct
gaaatgatgaaaaaacgccgcggcgaagtgttctacgcgcgtccggaattttgtactgat
aacggcgcgatgatcgcctatgccggaatggtgcggtttaaagcaggcgcgacggcggat
ctcggcgttagcgtgcgtccgcgctggccgctggcggagttaccggctgcgtaa
Protein Properties
Pfam Domain Function:Not Available
Protein Residues:337
Protein Molecular Weight:36008
Protein Theoretical pI:Not Available
PDB File:4WQ4
Signaling Regions:Not Available
Transmembrane Regions:Not Available
Protein Sequence:
>tRNA N6-adenosine threonylcarbamoyltransferase
MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVPELASRDHVRKTVPLIQ
AALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAWDVPAIPVHHMEGHLLAPM
LEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDDAAGEAFDKTAKLLGLDYPGG
PLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFAANTIRDNGTDDQTRADIARAFE
DAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLRAKLAEMMKKRRGEVFYARPEFCTD
NGAMIAYAGMVRFKAGATADLGVSVRPRWPLAELPAA
References
External Links:
ResourceLink
Uniprot ID:P05852
Uniprot Name:TSAD_ECOLI
PDB ID:4WQ4
CCDB:TSAD_ECOLI
General Reference:Not Available