Type-1 restriction enzyme EcoKI specificity protein (P05719)

Identification
Name:Type-1 restriction enzyme EcoKI specificity protein
Synonyms:
  • S.EcoKI
  • Type I restriction enzyme EcoKI specificity protein
  • S protein
Gene Name:hsdS
Enzyme Class:
Biological Properties
General Function:Involved in DNA binding
Specific Function:The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity. The EcoKI enzyme recognizes 5'- AACN(6)GTGC-3'
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
GO Classification:
Function
binding
DNA binding
nucleic acid binding
Process
cellular macromolecule metabolic process
DNA metabolic process
DNA modification
macromolecule metabolic process
metabolic process
Gene Properties
Blattner:b4348
Gene OrientationCounterclockwise
Centisome Percentage:98.67
Left Sequence End4578091
Right Sequence End4579485
Gene Sequence:
>1395 bp
ATGCCTGACGCTAAAAAACAGGGGCGGTCAAACAAGGCAATGACGTTTTTCGTCTGCTTC
CTTGCCGCTCTGGCGGGATTACTCTTTGGCCTGGATATCGGTGTAATTGCTGGCGCACTG
CCGTTTATTGCAGATGAATTCCAGATTACTTCGCACACGCAAGAATGGGTCGTAAGCTCC
ATGATGTTCGGTGCGGCAGTCGGTGCGGTGGGCAGCGGCTGGCTCTCCTTTAAACTCGGG
CGCAAAAAGAGCCTGATGATCGGCGCAATTTTGTTTGTTGCCGGTTCGCTGTTCTCTGCG
GCTGCGCCAAACGTTGAAGTACTGATTCTTTCCCGCGTTCTACTGGGGCTGGCGGTGGGT
GTGGCCTCTTATACCGCACCGCTGTACCTCTCTGAAATTGCGCCGGAAAAAATTCGTGGC
AGTATGATCTCGATGTATCAGTTGATGATCACTATCGGGATCCTCGGTGCTTATCTTTCT
GATACCGCCTTCAGCTACACCGGTGCATGGCGCTGGATGCTGGGTGTGATTATCATCCCG
GCAATTTTGCTGCTGATTGGTGTCTTCTTCCTGCCAGACAGCCCACGTTGGTTTGCCGCC
AAACGCCGTTTTGTTGATGCCGAACGCGTGCTGCTACGCCTGCGTGACACCAGCGCGGAA
GCGAAACGCGAACTGGATGAAATCCGTGAAAGTTTGCAGGTTAAACAGAGTGGCTGGGCG
CTGTTTAAAGAGAACAGCAACTTCCGCCGCGCGGTGTTCCTTGGCGTACTGTTGCAGGTA
ATGCAGCAATTCACCGGGATGAACGTCATCATGTATTACGCGCCGAAAATCTTCGAACTG
GCGGGTTATACCAACACTACCGAGCAAATGTGGGGGACCGTGATTGTCGGCCTGACCAAC
GTACTTGCCACCTTTATCGCAATCGGCCTTGTTGACCGCTGGGGACGTAAACCAACGCTA
ACGCTGGGCTTCCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATC
GGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTC
GGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTG
AAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATC
GTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGGTG
TATGCGGCTCTGAACGTACTGTTTATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAA
CACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATA
GGCGCTCACGATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:464
Protein Molecular Weight:51395
Protein Theoretical pI:10
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Type-1 restriction enzyme EcoKI specificity protein
MSAGKLPEGWVIAPVSTVTTLIRGVTYKKEQAINYLKDDYLPLIRANNIQNGKFDTTDLV
FVPKNLVKESQKISPEDIVIAMSSGSKSVVGKSAHQHLPFECSFGAFCGVLRPEKLIFSG
FIAHFTKSSLYRNKISSLSAGANINNIKPASFDLINIPIPPLAEQKIIAEKLDTLLAQVD
STKARFEQIPQILKRFRQAVLGGAVNGKLTEKWRNFEPQHSVFKKLNFESILTELRNGLS
SKPNESGVGHPILRISSVRAGHVDQNDIRFLECSESELNRHKLQDGDLLFTRYNGSLEFV
GVCGLLKKLQHQNLLYPDKLIRARLTKDALPEYIEIFFSSPSARNAMMNCVKTTSGQKGI
SGKDIKSQVVLLPPVKEQAEIVRRVEQLFAYADTIEKQVNNALARVNNLTQSILAKAFRG
ELTAQWRAENPDLISGENSAAALLEKIKAERAASGGKKASRKKS
References
External Links:
ResourceLink
Uniprot ID:P05719
Uniprot Name:T1SK_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675753
Ecogene ID:EG10460
Ecocyc:EG10460
ColiBase:b4348
Kegg Gene:b4348
EchoBASE ID:EB0455
CCDB:T1SK_ECOLI
BacMap:16132169
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Chen, A., Powell, L. M., Dryden, D. T., Murray, N. E., Brown, T. (1995). "Tyrosine 27 of the specificity polypeptide of EcoKI can be UV crosslinked to a bromodeoxyuridine-substituted DNA target sequence." Nucleic Acids Res 23:1177-1183. Pubmed: 7739896
  • Gough, J. A., Murray, N. E. (1983). "Sequence diversity among related genes for recognition of specific targets in DNA molecules." J Mol Biol 166:1-19. Pubmed: 6304321
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553