| Identification |
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| Name: | Chromosomal replication initiator protein DnaA |
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| Synonyms: | Not Available |
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| Gene Name: | dnaA |
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| Enzyme Class: | Not Available |
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| Biological Properties |
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| General Function: | transcription, DNA-templated |
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| Specific Function: | Plays a key role in the initiation and regulation of chromosomal replication. Binds in an ATP-dependent fashion to the origin of replication (oriC) to initiate formation of the DNA replication initiation complex exactly once per cell cycle. Binds the DnaA box (consensus sequence 5'-TTATC[CA]A[CA]A-3'); subsequent binding of DNA polymerase III subunits leads to replisome formation. The DnaA-ATP form converts to DnaA-ADP; once converted to ADP the protein cannot initiate replication, ensuring only 1 round of replication per cell cycle. DnaA can inhibit its own gene expression as well as that of other genes such as dam, rpoH, ftsA and mioC.Also required for replication of plasmid DNA; binds 4 dnaA boxes in the minimal plasmid RK2 replication origin (oriV). |
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| Cellular Location: | Not Available |
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| SMPDB Pathways: | Not Available |
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| KEGG Pathways: | |
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| Metabolites: | |
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| GO Classification: | | Function |
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| ATP binding | | cytoplasm | | DNA binding | | DNA replication | | DNA replication initiation | | DNA replication origin binding | | negative regulation of DNA replication | | plasma membrane | | regulation of DNA replication | | regulation of transcription, DNA-templated | | sequence-specific DNA binding | | transcription, DNA-templated |
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| Gene Properties |
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| Blattner: | Not Available |
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| Gene Orientation | Not Available |
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| Centisome Percentage: | Not Available |
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| Left Sequence End | Not Available |
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| Right Sequence End | Not Available |
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| Gene Sequence: | >1404
gtgtcactttcgctttggcagcagtgtcttgcccgattgcaggatgagttaccagccaca
gaattcagtatgtggatacgcccattgcaggcggaactgagcgataacacgctggccctg
tacgcgccaaaccgttttgtcctcgattgggtacgggacaagtaccttaataatatcaat
ggactgctaaccagtttctgcggagcggatgccccacagctgcgttttgaagtcggcacc
aaaccggtgacgcaaacgccacaagcggcagtgacgagcaacgtcgcggcccctgcacag
gtggcgcaaacgcagccgcaacgtgctgcgccttctacgcgctcaggttgggataacgtc
ccggccccggcagaaccgacctatcgttctaacgtaaacgtcaaacacacgtttgataac
ttcgttgaaggtaaatctaaccaactggcgcgcgcggcggctcgccaggtggcggataac
cctggcggtgcctataacccgttgttcctttatggcggcacgggtctgggtaaaactcac
ctgctgcatgcggtgggtaacggcattatggcgcgcaagccgaatgccaaagtggtttat
atgcactccgagcgctttgttcaggacatggttaaagccctgcaaaacaacgcgatcgaa
gagtttaaacgctactaccgttccgtagatgcactgctgatcgacgatattcagtttttt
gctaataaagaacgatctcaggaagagtttttccacaccttcaacgccctgctggaaggt
aatcaacagatcattctcacctcggatcgctatccgaaagagatcaacggcgttgaggat
cgtttgaaatcccgcttcggttggggactgactgtggcgatcgaaccgccagagctggaa
acccgtgtggcgatcctgatgaaaaaggccgacgaaaacgacattcgtttgccgggcgaa
gtggcgttctttatcgccaagcgtctacgatctaacgtacgtgagctggaaggggcgctg
aaccgcgtcattgccaatgccaactttaccggacgggcgatcaccatcgacttcgtgcgt
gaggcgctgcgcgacttgctggcattgcaggaaaaactggtcaccatcgacaatattcag
aagacggtggcggagtactacaagatcaaagtcgcggatctcctttccaagcgtcgatcc
cgctcggtggcgcgtccgcgccagatggcgatggcgctggcgaaagagctgactaaccac
agtctgccggagattggcgatgcgtttggtggccgtgaccacacgacggtgcttcatgcc
tgccgtaagatcgagcagttgcgtgaagagagccacgatatcaaagaagatttttcaaat
ttaatcagaacattgtcatcgtaa |
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| Protein Properties |
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| Pfam Domain Function: | Not Available |
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| Protein Residues: | 467 |
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| Protein Molecular Weight: | 52550 |
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| Protein Theoretical pI: | Not Available |
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| PDB File: | 1J1V |
| Signaling Regions: | Not Available |
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| Transmembrane Regions: | Not Available |
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| Protein Sequence: | >Chromosomal replication initiator protein DnaA
MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPNRFVLDWVRDKYLNNIN
GLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVAQTQPQRAAPSTRSGWDNV
PAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADNPGGAYNPLFLYGGTGLGKTH
LLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNAIEEFKRYYRSVDALLIDDIQFF
ANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEINGVEDRLKSRFGWGLTVAIEPPELE
TRVAILMKKADENDIRLPGEVAFFIAKRLRSNVRELEGALNRVIANANFTGRAITIDFVR
EALRDLLALQEKLVTIDNIQKTVAEYYKIKVADLLSKRRSRSVARPRQMAMALAKELTNH
SLPEIGDAFGGRDHTTVLHACRKIEQLREESHDIKEDFSNLIRTLSS |
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| References |
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| External Links: | |
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| General Reference: | Not Available |
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