Mannose-6-phosphate isomerase (P00946)

Identification
Name:Mannose-6-phosphate isomerase
Synonyms:
  • Phosphohexomutase
  • Phosphomannose isomerase
  • PMI
Gene Name:manA
Enzyme Class:
Biological Properties
General Function:Involved in mannose-6-phosphate isomerase activity
Specific Function:Involved in the conversion of glucose to GDP-L-fucose, which can be converted to L-fucose, a capsular polysaccharide
Cellular Location:Cytoplasm (Probable)
SMPDB Pathways:
  • Amino sugar and nucleotide sugar metabolism II PW000887
  • Mannose Metabolism PW000822
  • colanic acid building blocks biosynthesis PW000951
KEGG Pathways:
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb
1.0Mannose 6-phosphate ↔ 1.0beta-D-Fructose 6-phosphate
ReactionCard
SMPDB Reactions:
1.0α-D-mannose 6-phosphate ↔ 1.0Thumb
1.0α-D-mannose 6-phosphate ↔ 1.0D-tagatofuranose 6-phosphate
ReactionCard
1.0Thumb → 1.0α-D-mannose 6-phosphate
1.0D-tagatofuranose 6-phosphate → 1.0α-D-mannose 6-phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb
1.0Mannose 6-phosphate ↔ 1.0Fructose 6-phosphate
ReactionCard
Complex Reactions:
1.0Thumb → 1.0Thumb
1.0Mannose 6-phosphate → 1.0Fructose 6-phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB03971beta-D-Fructose 6-phosphateMetaboCard
ECMDB24205D-tagatofuranose 6-phosphateMetaboCard
ECMDB00124Fructose 6-phosphateMetaboCard
ECMDB01078Mannose 6-phosphateMetaboCard
GO Classification:
Function
binding
catalytic activity
cation binding
intramolecular oxidoreductase activity
intramolecular oxidoreductase activity, interconverting aldoses and ketoses
ion binding
isomerase activity
mannose-6-phosphate isomerase activity
metal ion binding
transition metal ion binding
zinc ion binding
Process
carbohydrate metabolic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b1613
Gene OrientationClockwise
Centisome Percentage:36.35
Left Sequence End1686600
Right Sequence End1687775
Gene Sequence:
>1176 bp
ATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCATGGCAACACTTGCGCCCAATCGC
TTTTTCTTTATGTCGCCGTACCGCAGTTTTACGACGTCAGGATGTTTCGCCCGCTTCGAT
GAACCGGCTGTGAACGGGGATTCGCCCGACAGTCCCTTCCAGCAAAAACTCGCCGCGCTG
TTTGCCGATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTC
GATCCACGTCAGCCTTCGTCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAG
GAAAAACAAGCTTCCGCACGCCGTTTCACCCGCAGCCAGTCGCTGAATGTGGTGGAACGC
CAGGCAATTCCGGAGCAAACCACGTTTGAACAGATGGTTGCCCGCGCCGCCGCACTTACC
GCCACGCCGCAGGTCGACAAAGTGGTGTTGTCACGGTTGATTGATATCACCACTGACGCC
GCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAACCCGGTTAGTTACAAC
TTCCATGTTCCGCTGGCTGATGGTGGCGTCCTGCTGGGGGCCAGCCCGGAACTGCTGCTA
CGTAAAGACGGCGAGCGTTTTAGCTCCATTCCGTTAGCCGGTTCCGCGCGTCGTCAGCCG
GATGAAGTGCTCGATCGCGAAGCAGGTAATCGTCTGCTGGCGTCAGAAAAAGATCGCCAT
GAACATGAACTGGTGACTCAGGCGATGAAAGAGGTACTGCGCGAACGCAGTAGTGAGTTA
CACGTTCCTTCTTCTCCACAGCTGATCACCACGCCGACGCTGTGGCATCTCGCAACTCCC
TTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCG
ACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAA
CCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGC
GAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCC
GGAGCGGGGATTGTGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAA
CTTTCTACCATGTTGAACGTTTTTGGATTGCATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:391
Protein Molecular Weight:42850
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Mannose-6-phosphate isomerase
MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRD
VIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNKHNSEIGFAKENAAGIPMD
AAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQPVAGAHPAIAHFLQQPDAERL
SELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPEDSGLFSPLLLNVVK
LNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQ
LLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETTISQQSAAILFCVEGDATLWKGSQQLQ
LKPGESAFIAANESPVTVKGHGRLARVYNKL
References
External Links:
ResourceLink
Uniprot ID:P00946
Uniprot Name:MANA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674714
Ecogene ID:EG10566
Ecocyc:EG10566
ColiBase:b1613
Kegg Gene:b1613
EchoBASE ID:EB0561
CCDB:MANA_ECOLI
BacMap:49176125
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Miles, J. S., Guest, J. R. (1984). "Nucleotide sequence and transcriptional start point of the phosphomannose isomerase gene (manA) of Escherichia coli." Gene 32:41-48. Pubmed: 6397402
  • Zhang, J., Sprung, R., Pei, J., Tan, X., Kim, S., Zhu, H., Liu, C. F., Grishin, N. V., Zhao, Y. (2009). "Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli." Mol Cell Proteomics 8:215-225. Pubmed: 18723842