UDP-N-acetylmuramate--L-alanine ligase (P17952)

Identification
Name:UDP-N-acetylmuramate--L-alanine ligase
Synonyms:
  • UDP-N-acetylmuramoyl-L-alanine synthetase
Gene Name:murC
Enzyme Class:
Biological Properties
General Function:Involved in ATP binding
Specific Function:Cell wall formation
Cellular Location:Cytoplasm (Probable)
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0L-Alanine + 1.0Adenosine triphosphate + 1.0UDP-N-Acetylmuraminate ↔ 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0UDP-N-Acetylmuramoyl-L-alanine
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0UDP-N-Acetylmuraminate + 1.0L-Alanine ↔ 1.0ADP + 1.0Phosphate + 1.0UDP-N-Acetylmuramoyl-L-alanine
ReactionCard
SMPDB Reactions:
1.0UDP-N-acetylmuraminate+1.0Thumb+1.0L-Alanine+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Adenosine diphosphate+1.0Thumb+1.0Thumb
1.0UDP-N-acetylmuraminate + 1.0Adenosine triphosphate + 1.0L-Alanine + 1.0UDP-N-Acetylmuraminate + 1.0L-Alanine → 1.0UDP-N-Acetylmuramoyl-L-alanine + 1.0Adenosine diphosphate + 1.0Phosphate + 1.0ADP
ReactionCard
1.0Thumb+1.0L-Alanine+1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0UDP-N-acetyl-α-D-muramate + 1.0L-Alanine + 1.0Adenosine triphosphate + 1.0L-Alanine → 1.0Adenosine diphosphate + 1.0Phosphate + 1.0Hydrogen ion + 1.0UDP-N-Acetylmuramoyl-L-alanine + 1.0ADP
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0UDP-N-acetyl-α-D-muramate + 1.0Adenosine triphosphate + 1.0L-Alanine → 1.0UDP-N-Acetylmuramyl-L-Ala + 1.0ADP + 1.0Phosphate + 1.0Hydrogen ion
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0L-Alanine + 1.0Adenosine triphosphate + 1.0UDP-N-Acetylmuraminate ↔ 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0UDP-N-Acetylmuramoyl-L-alanine
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0UDP-N-Acetylmuraminate + 1.0L-Alanine → 1.0ADP + 1.0Inorganic phosphate + 1.0UDP-N-Acetylmuramoyl-L-alanine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB00161L-AlanineMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB24238UDP-N-acetyl-α-D-muramateMetaboCard
ECMDB03266UDP-N-AcetylmuraminateMetaboCard
ECMDB04168UDP-N-Acetylmuramoyl-L-alanineMetaboCard
ECMDB20485UDP-N-Acetylmuramyl-L-AlaMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
acid-amino acid ligase activity
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
ligase activity
ligase activity, forming carbon-nitrogen bonds
nucleoside binding
purine nucleoside binding
UDP-N-acetylmuramate-L-alanine ligase activity
Process
aminoglycan metabolic process
biological regulation
biosynthetic process
carbohydrate metabolic process
cell division
cell wall biogenesis
cell wall organization or biogenesis
cellular cell wall organization or biogenesis
cellular component organization or biogenesis
cellular process
glycosaminoglycan metabolic process
metabolic process
peptidoglycan biosynthetic process
peptidoglycan metabolic process
peptidoglycan-based cell wall biogenesis
polysaccharide metabolic process
primary metabolic process
regulation of biological quality
regulation of cell shape
Gene Properties
Blattner:b0091
Gene OrientationClockwise
Centisome Percentage:2.17
Left Sequence End100765
Right Sequence End102240
Gene Sequence:
>1476 bp
ATGAATACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGG
CACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCC
AATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTA
ATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGC
GTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAA
GCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCAT
GGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATC
TACGCAGAAGCGGGGCTCGACCCAACCTTCGTTAACGGCGGGCTGGTAAAAGCGGCGGGG
GTTCATGCGCGTTTGGGGCATGGTCGGTACCTGATTGCCGAAGCAGATGAGAGTGATGCA
TCGTTCCTGCATCTGCAACCGATGGTGGCGATTGTCACCAATATCGAAGCCGACCACATG
GATACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAAC
CTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTA
CCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTA
GAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAG
CCGATGCGCGTCACCCTGAATGCGCCAGGTCGTCATAACGCGCTGAACGCCGCAGCTGCG
GTTGCGGTTGCTACGGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGC
TTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGAAT
GGTAAAAGCGGTACGGCAATGCTGGTCGATGACTACGGCCACCACCCGACGGAAGTGGAC
GCCACCATTAAAGCGGCGCGCGCAGGCTGGCCGGATAAAAACCTGGTAATGCTGTTTCAG
CCGCACCGTTTTACCCGTACGCGCGACCTGTATGATGATTTCGCCAATGTGCTGACGCAG
GTTGATACCCTGTTGATGCTGGAAGTGTATCCGGCTGGCGAAGCGCCAATTCCGGGAGCG
GACAGCCGTTCGCTGTGTCGCACAATTCGTGGACGTGGGAAAATTGATCCCATTCTGGTG
CCGGATCCGGCGCGGGTAGCCGAGATGCTGGCACCGGTATTAACCGGTAACGACCTGATT
CTCGTTCAGGGGGCTGGTAATATTGGAAAAATTGCCCGTTCTTTAGCTGAAATCAAACTG
AAGCCGCAAACTCCGGAGGAAGAACAACATGACTGA
Protein Properties
Pfam Domain Function:
Protein Residues:491
Protein Molecular Weight:53626
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>UDP-N-acetylmuramate--L-alanine ligase
MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGYQISGSDLAPNPVTQQL
MNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEARIPVIRRAEMLAELMRFRH
GIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAGVHARLGHGRYLIAEADESDA
SFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFLHNLPFYGRAVMCVDDPVIRELL
PRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLRQDKEPMRVTLNAPGRHNALNAAAA
VAVATEEGIDDEAILRALESFQGTGRRFDFLGEFPLEPVNGKSGTAMLVDDYGHHPTEVD
ATIKAARAGWPDKNLVMLFQPHRFTRTRDLYDDFANVLTQVDTLLMLEVYPAGEAPIPGA
DSRSLCRTIRGRGKIDPILVPDPARVAEMLAPVLTGNDLILVQGAGNIGKIARSLAEIKL
KPQTPEEEQHD
References
External Links:
ResourceLink
Uniprot ID:P17952
Uniprot Name:MURC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321972
Ecogene ID:EG10619
Ecocyc:EG10619
ColiBase:b0091
Kegg Gene:b0091
EchoBASE ID:EB0614
CCDB:MURC_ECOLI
BacMap:16128084
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Eveland, S. S., Pompliano, D. L., Anderson, M. S. (1997). "Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-gamma-glutamate ligases: identification of a ligase superfamily." Biochemistry 36:6223-6229. Pubmed: 9166795
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Ikeda, M., Wachi, M., Jung, H. K., Ishino, F., Matsuhashi, M. (1990). "Nucleotide sequence involving murG and murC in the mra gene cluster region of Escherichia coli." Nucleic Acids Res 18:4014. Pubmed: 2197603
  • Liger, D., Masson, A., Blanot, D., van Heijenoort, J., Parquet, C. (1995). "Over-production, purification and properties of the uridine-diphosphate-N-acetylmuramate:L-alanine ligase from Escherichia coli." Eur J Biochem 230:80-87. Pubmed: 7601127
  • Yura, T., Mori, H., Nagai, H., Nagata, T., Ishihama, A., Fujita, N., Isono, K., Mizobuchi, K., Nakata, A. (1992). "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Nucleic Acids Res 20:3305-3308. Pubmed: 1630901