Canmetcon

Escherichia coli Statistics


Length of cell 2 mm
Width of cell 1 mm
Volume of cell 1 mm3
Volume of cell 1 x 10-15 L
Volume of cell occupied by ribosomes 1 x 108 nm3 = 10% of cell
Volume of cell occupied by water 7 x 108 nm3
Area of cell 6 mm2 or 6 x 10-12 m2
Cell wet weight 1 x 10-12 g
Cell dry weight 3 x 10-13 g
Periplasm volume 6.5 x 10-17 L
Cytoplasm volume 6.7 x 10-16 L
Surface area of a lipid molecule 0.5 nm2
Total protein mass in cell 15 x10-12 g
Total mass of water in cell 7 x 10-13 g
Number of proteins in cell 3 x 106
Number of cytoplasmic proteins 2 x 106
Number of membrane proteins 1 x 106
Number of ribosomes in cell 20,000
Number of tRNA molecules 200,000
Number of mRNA molecules 1400
Number of lipids in cell 20,000,000-22,000,000 = 50 mM
Number of Phosphatidylethanolamine lipids 14,000,0000-15,400,000 = 35.5 mM
Number of Phosphatidylglycerol lipids 2,200,000-4,000,000 = 6-10 mM
Number of Cardiolipin lipids 1,400,000 = 3.5 mM
Number of Phosphatidylserine lipids 1,000,000 = 2.5 mM
Number of LPS molecules in cell 1,200,000 - 2,000,000 = 3-5 mM
Number of water molecules in cell 2 x 1010
Number of ions in cell 6 x 107
Circumference/Length of chromosome 1.55 mm
Concentration of protein in cell 5-8 mM
Concentration of RNA in cell 0.5-0.8 mM
Concentration of DNA in cell 2 nM
% Volume occupied by water 70
% Volume occupied by protein 17
% Volume occupied by rRNA 5
% Volume occupied by tRNA 0.8
% Volume occupied by DNA 1
% Volume occupied by mRNA 0.2
% Volume occupied by ribosomes 8
0.4 molecules/cell1 nM concentration
CompoundConcentrationNumber of Molecules per Cell
K 200-250 mM 80,000,000-100,000,000
Na 5 mM 2,000,000
Ca 6 mM 2,300,000
Cl 6 mM 2,400,000
Mg 10 mM 4,000,000
Fe 18 mM 7,000,000
Mn 4 mM 1,700,000
Zn 4 mM 1,700,000
Mo 4 mM 1,700,000
Cu 4 mM 1,700,000
PO4 5 mM 2,000,000
Glucose 0.5 mM 200,000
Phosphoenol pyruvate 2.8 mM 1,100,000
Pyruvate 0.9 mM 370,000
Glucose-6-PO4 0.05 mM 20,000
ATP 1.3-7.0 mM 500,000-1,700,000
ADP 0.17 mM 70,000
NADP 0.63 mM 240,000
NADPH 0.56 mM 220,000
Glycerol 3-phosphate 100-200 uM 40,000-80,000

References

1. Cybercell Database: http://ccdb.wishartlab.com/CCDB/cgi-bin/STAT_NEW.cgi
2. Phillips R., Kondev, J., Theriot, J. (2008) “Physical Biology of the Cell” Garland Science, New York, NY.