Identification
Name:Diacylglycerol kinase
Synonyms:
  • DAGK
  • Diglyceride kinase
  • DGK
Gene Name:dgkA
Enzyme Class:
Biological Properties
General Function:Involved in diacylglycerol kinase activity
Specific Function:Recycling of diacylglycerol produced during the turnover of membrane phospholipid
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.01,2-Diacyl-sn-glycerol+1.01,2-Diacyl-sn-glycerol1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.01,2-Diacyl-sn-glycerol + 1.01,2-Diacyl-sn-glycerol ↔ 1.0ADP + 1.0PA(16:0/16:0)
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.01,2-diacylglycerol1.0Thumb+1.01,2-diacyl-sn-glycerol 3-phosphate
1.0Adenosine triphosphate + 1.01,2-diacylglycerol → 1.0ADP + 1.01,2-diacyl-sn-glycerol 3-phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB211031,2-Diacyl-sn-glycerol (didodecanoyl, n-C12:0)MetaboCard
ECMDB211041,2-Diacyl-sn-glycerol (dihexadec-9-enoyl, n-C16:1)MetaboCard
ECMDB211051,2-Diacyl-sn-glycerol (dihexadecanoyl, n-C16:0)MetaboCard
ECMDB211061,2-Diacyl-sn-glycerol (dioctadec-11-enoyl, n-C18:1)MetaboCard
ECMDB211081,2-Diacyl-sn-glycerol (ditetradec-7-enoyl, n-C14:1)MetaboCard
ECMDB211091,2-Diacyl-sn-glycerol (ditetradecanoyl, n-C14:0)MetaboCard
ECMDB214661,2-Dioctadecanoyl-sn-glycerolMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB23241DG(10:0/10:0/0:0)MetaboCard
ECMDB23242DG(10:0/12:0/0:0)MetaboCard
ECMDB23243DG(10:0/14:0/0:0)MetaboCard
ECMDB23244DG(10:0/15:0/0:0)MetaboCard
ECMDB23245DG(10:0/16:0/0:0)MetaboCard
ECMDB23246DG(10:0/16:1(9Z)/0:0)MetaboCard
ECMDB23247DG(10:0/18:0/0:0)MetaboCard
ECMDB23248DG(10:0/18:1(9Z)/0:0)MetaboCard
ECMDB23249DG(10:0/19:1(9Z)/0:0)MetaboCard
ECMDB23250DG(12:0/10:0/0:0)MetaboCard
ECMDB23251DG(12:0/12:0/0:0)MetaboCard
ECMDB23252DG(12:0/14:0/0:0)MetaboCard
ECMDB23253DG(12:0/15:0/0:0)MetaboCard
ECMDB23254DG(12:0/16:0/0:0)MetaboCard
ECMDB23255DG(12:0/16:1(9Z)/0:0)MetaboCard
ECMDB23256DG(12:0/18:0/0:0)MetaboCard
ECMDB23257DG(12:0/18:1(9Z)/0:0)MetaboCard
ECMDB23258DG(12:0/19:1(9Z)/0:0)MetaboCard
ECMDB23259DG(14:0/10:0/0:0)MetaboCard
ECMDB23260DG(14:0/12:0/0:0)MetaboCard
ECMDB23261DG(14:0/14:0/0:0)MetaboCard
ECMDB23262DG(14:0/15:0/0:0)MetaboCard
ECMDB23263DG(14:0/16:0/0:0)MetaboCard
ECMDB23264DG(14:0/16:1(9Z)/0:0)MetaboCard
ECMDB23265DG(14:0/18:0/0:0)MetaboCard
ECMDB23266DG(14:0/18:1(9Z)/0:0)MetaboCard
ECMDB23267DG(14:0/19:1(9Z)/0:0)MetaboCard
ECMDB23268DG(15:0/10:0/0:0)MetaboCard
ECMDB23269DG(15:0/12:0/0:0)MetaboCard
ECMDB23270DG(15:0/14:0/0:0)MetaboCard
ECMDB23271DG(15:0/15:0/0:0)MetaboCard
ECMDB23272DG(15:0/16:0/0:0)MetaboCard
ECMDB23273DG(15:0/16:1(9Z)/0:0)MetaboCard
ECMDB23274DG(15:0/18:0/0:0)MetaboCard
ECMDB23275DG(15:0/18:1(9Z)/0:0)MetaboCard
ECMDB23276DG(15:0/19:1(9Z)/0:0)MetaboCard
ECMDB23277DG(16:0/10:0/0:0)MetaboCard
ECMDB23278DG(16:0/12:0/0:0)MetaboCard
ECMDB23279DG(16:0/14:0/0:0)MetaboCard
ECMDB23280DG(16:0/15:0/0:0)MetaboCard
ECMDB23281DG(16:0/16:0/0:0)MetaboCard
ECMDB23282DG(16:0/16:1(9Z)/0:0)MetaboCard
ECMDB23283DG(16:0/18:0/0:0)MetaboCard
ECMDB23284DG(16:0/18:1(9Z)/0:0)MetaboCard
ECMDB23285DG(16:0/19:1(9Z)/0:0)MetaboCard
ECMDB23286DG(16:1(9Z)/10:0/0:0)MetaboCard
ECMDB23287DG(16:1(9Z)/12:0/0:0)MetaboCard
ECMDB23288DG(16:1(9Z)/14:0/0:0)MetaboCard
ECMDB23289DG(16:1(9Z)/15:0/0:0)MetaboCard
ECMDB23290DG(16:1(9Z)/16:0/0:0)MetaboCard
ECMDB23291DG(16:1(9Z)/16:1(9Z)/0:0)MetaboCard
ECMDB23292DG(16:1(9Z)/18:0/0:0)MetaboCard
ECMDB23293DG(16:1(9Z)/18:1(9Z)/0:0)MetaboCard
ECMDB23294DG(16:1(9Z)/19:1(9Z)/0:0)MetaboCard
ECMDB23295DG(18:0/10:0/0:0)MetaboCard
ECMDB23296DG(18:0/12:0/0:0)MetaboCard
ECMDB23297DG(18:0/14:0/0:0)MetaboCard
ECMDB23298DG(18:0/15:0/0:0)MetaboCard
ECMDB23299DG(18:0/16:0/0:0)MetaboCard
ECMDB23300DG(18:0/16:1(9Z)/0:0)MetaboCard
ECMDB23301DG(18:0/18:0/0:0)MetaboCard
ECMDB23302DG(18:0/18:1(9Z)/0:0)MetaboCard
ECMDB23303DG(18:0/19:1(9Z)/0:0)MetaboCard
ECMDB23304DG(18:1(9Z)/10:0/0:0)MetaboCard
ECMDB23305DG(18:1(9Z)/12:0/0:0)MetaboCard
ECMDB23306DG(18:1(9Z)/14:0/0:0)MetaboCard
ECMDB23307DG(18:1(9Z)/15:0/0:0)MetaboCard
ECMDB23308DG(18:1(9Z)/16:0/0:0)MetaboCard
ECMDB23309DG(18:1(9Z)/16:1(9Z)/0:0)MetaboCard
ECMDB23310DG(18:1(9Z)/18:0/0:0)MetaboCard
ECMDB23311DG(18:1(9Z)/18:1(9Z)/0:0)MetaboCard
ECMDB23312DG(18:1(9Z)/19:1(9Z)/0:0)MetaboCard
ECMDB23313DG(19:1(9Z)/10:0/0:0)MetaboCard
ECMDB23314DG(19:1(9Z)/12:0/0:0)MetaboCard
ECMDB23315DG(19:1(9Z)/14:0/0:0)MetaboCard
ECMDB23316DG(19:1(9Z)/15:0/0:0)MetaboCard
ECMDB23317DG(19:1(9Z)/16:0/0:0)MetaboCard
ECMDB23318DG(19:1(9Z)/16:1(9Z)/0:0)MetaboCard
ECMDB23319DG(19:1(9Z)/18:0/0:0)MetaboCard
ECMDB23320DG(19:1(9Z)/18:1(9Z)/0:0)MetaboCard
ECMDB23321DG(19:1(9Z)/19:1(9Z)/0:0)MetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00674PA(16:0/16:0)MetaboCard
GO Classification:
Component
cell part
membrane
Function
catalytic activity
diacylglycerol kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
metabolic process
organophosphate metabolic process
phospholipid biosynthetic process
phospholipid metabolic process
Gene Properties
Blattner:b4042
Gene OrientationClockwise
Centisome Percentage:91.70
Left Sequence End4254660
Right Sequence End4255028
Gene Sequence:
>369 bp
ATGAAATATAGTTCAATATTTTCGATGCTTTCATTTTTTATACTATTTGCCTGTAATGAG
ACAGCTGTTTACGGTTCTGATGAAAACATTATTTTTATGAGGTATGTGGAAAAATTACAT
TTAGATAAATACTCTGTTAAAAATACGGTAAAAACTGAAACAATGGCGATACAATTAGCT
GAAATATATGTTAGGTATCGCTATGGCGAACGGATTGCAGAAGAAGAAAAACCATATTTA
ATTACGGAACTACCAGATAGTTGGGTTGTTGAGGGAGCAAAGTTACCTTATGAAGTTGCG
GGTGGTGTATTTATTATAGAAATTAATAAGAAAAATGGATGTGTTTTGAATTTCCTACAT
AGTAAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:122
Protein Molecular Weight:13245
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • 34-49
  • 53-69
  • 96-119
Protein Sequence:
>Diacylglycerol kinase
MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVVIACWLDVDAITRVLLI
SSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAVLIAIIVAVITWCILLWSH
FG
References
External Links:
ResourceLink
Uniprot ID:P0ABN1
Uniprot Name:KDGL_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674637
Ecogene ID:EG10224
Ecocyc:EG10224
ColiBase:b4042
Kegg Gene:b4042
EchoBASE ID:EB0220
CCDB:KDGL_ECOLI
BacMap:16131868
General Reference:
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lightner, V. A., Bell, R. M., Modrich, P. (1983). "The DNA sequences encoding plsB and dgk loci of Escherichia coli." J Biol Chem 258:10856-10861. Pubmed: 6309817
  • Loomis, C. R., Walsh, J. P., Bell, R. M. (1985). "sn-1,2-Diacylglycerol kinase of Escherichia coli. Purification, reconstitution, and partial amino- and carboxyl-terminal analysis." J Biol Chem 260:4091-4097. Pubmed: 2984194
  • Smith, R. L., O'Toole, J. F., Maguire, M. E., Sanders, C. R. 2nd (1994). "Membrane topology of Escherichia coli diacylglycerol kinase." J Bacteriol 176:5459-5465. Pubmed: 8071224