Identification
Name:3-phenylpropionate/cinnamic acid dioxygenase subunit beta
Synonyms:
  • Digoxigenin subunit beta
Gene Name:hcaF
Enzyme Class:
Biological Properties
General Function:Involved in 3-phenylpropionate dioxygenase activity
Specific Function:Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Microbial metabolism in diverse environments ec01120
  • Phenylalanine metabolism ec00360
KEGG Reactions:
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SMPDB Reactions:
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EcoCyc Reactions:
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Complex Reactions:
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Metabolites:
ECMDB IDNameView
ECMDB21422Cinnamic acidMetaboCard
ECMDB20129cis-3-(3-Carboxyethenyl)-3,5-cyclohexadiene-1,2-diolMetaboCard
ECMDB21320Cis-3-(3-carboxyethyl)-3,5-cyclohexadiene-1,2-diolMetaboCard
ECMDB20130cis-3-(Carboxy-ethyl)-3,5-cyclo-hexadiene-1,2-diolMetaboCard
ECMDB00764Hydrocinnamic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB04124OxygenMetaboCard
ECMDB20197Trans-2,3-DihydroxycinnamateMetaboCard
ECMDB00930trans-Cinnamic acidMetaboCard
GO Classification:
Function
catalytic activity
Process
cellular aromatic compound metabolic process
cellular metabolic process
metabolic process
oxidation reduction
Gene Properties
Blattner:b2539
Gene OrientationClockwise
Centisome Percentage:57.51
Left Sequence End2668412
Right Sequence End2668930
Gene Sequence:
>519 bp
ATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCG
TGGCATCTACTTGCTGTCGGATTCGGAAGTGGATTAAGCCCGATCGTTCCTGGGACGATG
GGCTCGCTGGCAGCGATTCCGTTCTGGTATCTGATGACCTTTTTGCCCTGGCAGCTCTAC
TCGCTGGTGGTGATGCTGGGGATCTGTATCGGCGTCTATCTTTGTCATCAAACGGCGAAA
GACATGGGTGTGCACGATCATGGCAGCATTGTCTGGGACGAATTTATTGGTATGTGGATC
ACGCTCATGGCGCTGCCGACCAATGACTGGCAGTGGGTTGCCGCCGGGTTTGTGATTTTC
CGTATTCTGGATATGTGGAAGCCGTGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGC
GGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTAT
TTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAG
Protein Properties
Pfam Domain Function:
Protein Residues:172
Protein Molecular Weight:20579
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>3-phenylpropionate/cinnamic acid dioxygenase subunit beta
MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTMRTTVNAQTRDRRKGVQ
PPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNCQISETDIPNVFAVRVNYL
LYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQAVITSHNLSVLF
References
External Links:
ResourceLink
Uniprot ID:Q47140
Uniprot Name:HCAF_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674558
Ecogene ID:EG13457
Ecocyc:EG13457
ColiBase:b2539
Kegg Gene:b2539
EchoBASE ID:EB3230
CCDB:HCAF_ECOLI
BacMap:16130464
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Diaz, E., Ferrandez, A., Garcia, J. L. (1998). "Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12." J Bacteriol 180:2915-2923. Pubmed: 9603882
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837