Identification
Name:Probable enoyl-CoA hydratase paaG
Synonyms:Not Available
Gene Name:paaG
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Could possibly oxidize fatty acids using specific components
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
Thumb+ThumbThumb+Thumb
2-(1,2-Epoxy-1,2-dihydrophenyl)acetyl-CoA+ThumbThumb
Thumb+ThumbThumb+Thumb
(3S)-3-Hydroxyacyl-CoAThumb+trans-3-Enoyl-CoA+Thumb
(3S)-3-Hydroxyacyl-CoA ↔ trans-2,3-Dehydroacyl-CoA + trans-3-Enoyl-CoA + Water
ReactionCard
SMPDB Reactions:
ThumbThumb
2,3-didehydroadipyl-CoA+ThumbThumb
2,3-didehydroadipyl-CoA + Water3-Hydroxyadipyl-CoA
ReactionCard
EcoCyc Reactions:
2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoAThumb
2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA ↔ 2-Oxepin-2(3H)-ylideneacetyl-CoA
ReactionCard
Complex Reactions:
ThumbThumb
Metabolites:
ECMDB IDNameView
ECMDB231592-(1,2-Epoxy-1,2-dihydrophenyl)acetyl-CoAMetaboCard
ECMDB211642-Oxepin-2(3H)-ylideneacetyl-CoAMetaboCard
ECMDB202313-Hydroxyadipyl-CoAMetaboCard
ECMDB211723-Oxo-5,6-dehydrosuberyl-CoAMetaboCard
ECMDB201015-Carboxy-2-pentenoyl-CoAMetaboCard
ECMDB01206Acetyl-CoAMetaboCard
ECMDB01423Coenzyme AMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
Process
metabolic process
Gene Properties
Blattner:b1394
Gene OrientationClockwise
Centisome Percentage:31.39
Left Sequence End1456288
Right Sequence End1457076
Gene Sequence:
>789 bp
GTGATTGATAAATCCGCCTTTGTGCATCCAACCGCCATTGTGGAAGAGGGCGCGTCAATT
GGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAG
GGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAAT
GAGATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAA
CCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGT
GGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAAC
GCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACG
CTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCAT
CAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGTGTGGCGCAGGAC
GTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAA
GGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAG
CTGATTTATCGTAGCGGTAAAACGCTCGATGAAGTGAAACCGGAAATTGCTGAACTGGCG
GAAACATATCCGGAAGTGAAAGCCTTTACCGATTTCTTTGCACGCTCAACGCGCGGTCTG
ATTCGTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:262
Protein Molecular Weight:28404
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Probable enoyl-CoA hydratase paaG
MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVERDDTIRCLLLTGAGRGF
CAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPVICAVNGVAAGAGATLALG
GDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARAMGLALLGNQLSAEQAHEWGM
IWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINSAETNTLDTQLDLERDYQRLAGR
SADYREGVSAFLAKRSPQFTGK
References
External Links:
ResourceLink
Uniprot ID:P77467
Uniprot Name:PAAG_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674370
Ecogene ID:EG13741
Ecocyc:EG13741
ColiBase:b1394
Kegg Gene:b1394
EchoBASE ID:EB3504
CCDB:PAAG_ECOLI
BacMap:16129355
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Ferrandez, A., Garcia, J. L., Diaz, E. (2000). "Transcriptional regulation of the divergent paa catabolic operons for phenylacetic acid degradation in Escherichia coli." J Biol Chem 275:12214-12222. Pubmed: 10766858
  • Ferrandez, A., Minambres, B., Garcia, B., Olivera, E. R., Luengo, J. M., Garcia, J. L., Diaz, E. (1998). "Catabolism of phenylacetic acid in Escherichia coli. Characterization of a new aerobic hybrid pathway." J Biol Chem 273:25974-25986. Pubmed: 9748275
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553