N-acetylmannosamine kinase (P45425)

Identification
Name:N-acetylmannosamine kinase
Synonyms:
  • ManNAc kinase
  • N-acetyl-D-mannosamine kinase
Gene Name:nanK
Enzyme Class:
Biological Properties
General Function:Involved in ATP binding
Specific Function:Catalyzes the phosphorylation of N-acetylmannosamine (ManNAc) to ManNAc-6-P. Has also low level glucokinase activity in vitro
Cellular Location:Not Available
SMPDB Pathways:
  • N-acetylneuraminate and N-acetylmannosamine and N-acetylglucosamine degradation PW002030
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0N-Acetylmannosamine + 1.0Adenosine triphosphate → 1.0N-Acetyl-D-mannosamine 6-phosphate + 1.0ADP + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0N-Acetylmannosamine ↔ 1.0ADP + 1.0N-Acetyl-D-mannosamine 6-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0N-Acyl-D-mannosamine 6-phosphate
1.0Adenosine triphosphate + 1.0N-Acyl-D-mannosamine ↔ 1.0ADP + 1.0N-Acyl-D-mannosamine 6-phosphate
ReactionCard
SMPDB Reactions:
1.0N-Acetylmannosamine+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0N-Acetylmannosamine + 1.0Adenosine triphosphate → 1.0ADP + 1.0Hydrogen ion + 1.0N-Acetyl-D-mannosamine 6-phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0N-Acetylmannosamine + 1.0Adenosine triphosphate → 1.0N-Acetyl-D-mannosamine 6-phosphate + 1.0ADP + 1.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01121N-Acetyl-D-mannosamine 6-phosphateMetaboCard
ECMDB01129N-AcetylmannosamineMetaboCard
GO Classification:Not Available
Gene Properties
Blattner:b3222
Gene OrientationCounterclockwise
Centisome Percentage:72.58
Left Sequence End3367497
Right Sequence End3368372
Gene Sequence:
>876 bp
ATGAGCGAAAAGCTACAGAAAGTGCTGGCGCGTGCCGGCCACGGTTCTCGCCGTGAAATC
GAATCTATTATTGAAGCCGGTCGTGTGAGTGTTGATGGCAAAATTGCTAAACTCGGCGAT
CGTGTTGAAGTTACCCCTGGATTGAAAATCCGTATCGATGGTCACCTGATTTCGGTACGT
GAGTCCGCTGAACAAATTTGTCGCGTTCTGGCCTATTACAAACCGGAAGGTGAGTTGTGT
ACCCGTAACGATCCGGAAGGACGTCCAACGGTGTTTGACCGTCTGCCAAAACTGCGTGGC
GCACGCTGGATTGCCGTGGGTCGCTTGGACGTTAATACCTGTGGTCTGCTGCTGTTCACC
ACCGATGGTGAACTGGCAAACCGTTTAATGCACCCAAGCCGTGAAGTTGAACGTGAATAT
GCCGTGCGTGTATTTGGTCAGGTTGACGACGCGAAACTGCGTGATTTGAGTCGTGGCGTG
CAGTTGGAAGATGGTCCGGCAGCTTTTAAAACCATCAAGTTCAGCGGCGGCGAAGGGATC
AACCAGTGGTACAACGTGACTCTGACCGAAGGCCGTAACCGTGAAGTTCGTCGTCTGTGG
GAAGCGGTTGGTGTGCAGGTGAGCCGCCTGATCCGTGTTCGTTACGGTGATATCCCACTG
CCAAAAGGTCTGCCACGCGGTGGCTGGACGGAGCTGGACCTCGCCCAGACTAACTATCTG
CGCGAACTGGTGGAGCTACCGCCAGAAACCAGCTCTAAAGTCGCTGTAGAAAAAGACCGT
CGTCGCATGAAGGCGAATCAGATTCGTCGTGCGGTGAAACGTCACAGTCAGGTGAGCGGC
GGTCGTCGTTCTGGCGGACGTAATAATAACGGTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:291
Protein Molecular Weight:29644
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>N-acetylmannosamine kinase
MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDALSALVSPLQAHAQRVA
IASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIAINDAQAAAWAEFQALDGD
ITDMVFITVSTGVGGGVVSGCKLLTGPGGLAGHIGHTLADPHGPVCGCGRTGCVEAIASG
RGIAAAAQGELAGADAKTIFTRAGQGDEQAQQLIHRSARTLARLIADIKATTDCQCVVVG
GSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHDAGLLGAALLAQGEKL
References
External Links:
ResourceLink
Uniprot ID:P45425
Uniprot Name:NANK_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742064
Ecogene ID:EG12815
Ecocyc:EG12815
ColiBase:b3222
Kegg Gene:b3222
EchoBASE ID:EB2666
CCDB:NANK_ECOLI
BacMap:90111558
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Larion, M., Moore, L. B., Thompson, S. M., Miller, B. G. (2007). "Divergent evolution of function in the ROK sugar kinase superfamily: role of enzyme loops in substrate specificity." Biochemistry 46:13564-13572. Pubmed: 17979299
  • Miller, B. G., Raines, R. T. (2004). "Identifying latent enzyme activities: substrate ambiguity within modern bacterial sugar kinases." Biochemistry 43:6387-6392. Pubmed: 15157072
  • Plumbridge, J., Vimr, E. (1999). "Convergent pathways for utilization of the amino sugars N-acetylglucosamine, N-acetylmannosamine, and N-acetylneuraminic acid by Escherichia coli." J Bacteriol 181:47-54. Pubmed: 9864311