ECMDB: Carbamate kinase (P37306)

Identification

Name:

Carbamate kinase

Synonyms:

Not Available

Gene Name:

arcC

Enzyme Class:

2.7.2.2

Biological Properties

General Function:

Involved in cellular amino acid biosynthetic process

Specific Function:

ATP + NH(3) + CO(2) = ADP + carbamoyl phosphate

Cellular Location:

Cytoplasm (Potential)

SMPDB Pathways:

Nitrogen metabolism PW000755
allantoin degradation (anaerobic) PW002050

KEGG Pathways:

Arginine and proline metabolism ec00330
Microbial metabolism in diverse environments ec01120
Nitrogen metabolism ec00910
Purine metabolism ec00230

KEGG Reactions:

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Ammonia + 1.0Carbon dioxide ↔ 1.0ADP + 1.0Carbamoylphosphate
ReactionCard

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Carbamic acid ↔ 1.0ADP + 1.0Carbamoylphosphate
ReactionCard

1.0

←

1.0

1.0Ammonia + 1.0Carbon dioxide + 1.0Adenosine triphosphate ← 1.0Hydrogen ion + 1.0Carbamoylphosphate + 1.0ADP
ReactionCard

SMPDB Reactions:

1.0

→

1.0

1.0Carbon dioxide + 1.0Water → 1.0Carbamic acid
ReactionCard

1.0

2.0

→

1.0

1.0Carbamoylphosphate + 1.0ADP + 2.0Hydrogen ion → 1.0Ammonium + 1.0Adenosine triphosphate + 1.0Carbon dioxide
ReactionCard

EcoCyc Reactions:

1.0

←

1.0

1.0Ammonia + 1.0Carbon dioxide + 1.0Adenosine triphosphate ← 1.0Hydrogen ion + 1.0Carbamoylphosphate + 1.0ADP
ReactionCard

Complex Reactions:

1.0

↔

1.0

2.0

1.0Adenosine triphosphate + 1.0Carbon dioxide + 1.0Ammonium ↔ 1.0ADP + 1.0Carbamoylphosphate + 2.0Hydrogen ion
ReactionCard

1.0

→

1.0

1.0Adenosine triphosphate + 1.0Ammonia + 1.0Carbon dioxide → 1.0ADP + 1.0Carbamoylphosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00538	Adenosine triphosphate	MetaboCard
ECMDB01341	ADP	MetaboCard
ECMDB00051	Ammonia	MetaboCard
ECMDB21186	Ammonium	MetaboCard
ECMDB03551	Carbamic acid	MetaboCard
ECMDB01096	Carbamoylphosphate	MetaboCard
ECMDB04030	Carbon dioxide	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
carbamate kinase activity
catalytic activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
arginine metabolic process
cellular amino acid and derivative metabolic process
cellular amino acid biosynthetic process
cellular amino acid metabolic process
cellular metabolic process
glutamine family amino acid metabolic process
metabolic process

Gene Properties

Blattner:

b0521

Gene Orientation

Clockwise

Centisome Percentage:

11.85

Left Sequence End

549662

Right Sequence End

550555

Gene Sequence:

>894 bp
ATGAAAACACTGGTTGTGGCTCTTGGGGGCAACGCCTTACTCCAGCGCGGTGAGGCGCTG
ACGGCAGAAAATCAATATCGCAATATCGCCAGTGCTGTACCCGCGCTGGCACGCCTGGCC
CGTTCTTATCGGTTGGCGATTGTTCACGGCAACGGGCCGCAGGTGGGGCTGCTGGCATTG
CAGAATCTGGCGTGGAAAGAGGTAGAACCGTATCCGCTGGATGTGCTGGTTGCGGAAAGC
CAGGGGATGATTGGCTATATGCTGGCGCAGAGTTTGAGCGCACAGCCGCAGATGCCGCCC
GTGACGACGGTGCTGACGCGCATTGAGGTTTCGCCTGATGATCCGGCGTTTTTGCAGCCA
GAGAAATTTATTGGTCCGGTTTATCAGCCAGAAGAACAAGAGGCACTGGAAGCGGCTTAC
GGCTGGCAGATGAAACGTGATGGTAAATATTTGCGCCGGGTGGTGGCGTCTCCGCAACCG
CGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATT
TGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATT
GATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATC
CTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGC
CATGCCACACCGGATGAGTTAGCGCCATTTGCCAAAGCCGATGGTTCGATGGGGCCGAAT
GTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTA
TCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAG

Protein Properties

Pfam Domain Function:

AA_kinase (PF00696 )

Protein Residues:

297

Protein Molecular Weight:

31644

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Carbamate kinase
MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYRLAIVHGNGPQVGLLAL
QNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVTTVLTRIEVSPDDPAFLQP
EKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQPRKILDSEAIELLLKEGHVVI
CSGGGGVPVTDDGAGSEAVIDKDLAAALLAEQINADGLVILTDADAVYENWGTPQQRAIR
HATPDELAPFAKADGSMGPNVTAVSGYVRSRGKPAWIGALSRIEETLAGEAGTCISL

References

External Links:

Resource	Link
Uniprot ID:	P37306
Uniprot Name:	ARCC_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674658
Ecogene ID:	EG12384
Ecocyc:	EG12384
ColiBase:	b0521
Kegg Gene:	b0521
EchoBASE ID:	EB2285
CCDB:	ARCC_ECOLI
BacMap:	16128505

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Borodovsky, M., Koonin, E. V., Rudd, K. E. (1994). "New genes in old sequence: a strategy for finding genes in the bacterial genome." Trends Biochem Sci 19:309-313. Pubmed: 7940673
Borodovsky, M., Rudd, K. E., Koonin, E. V. (1994). "Intrinsic and extrinsic approaches for detecting genes in a bacterial genome." Nucleic Acids Res 22:4756-4767. Pubmed: 7984428
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Robison, K., Gilbert, W., Church, G. M. (1994). "Large scale bacterial gene discovery by similarity search." Nat Genet 7:205-214. Pubmed: 7920643
Tiedeman, A. A., Keyhani, J., Kamholz, J., Daum, H. A. 3rd, Gots, J. S., Smith, J. M. (1989). "Nucleotide sequence analysis of the purEK operon encoding 5'-phosphoribosyl-5-aminoimidazole carboxylase of Escherichia coli K-12." J Bacteriol 171:205-212. Pubmed: 2464576
Watanabe, W., Sampei, G., Aiba, A., Mizobuchi, K. (1989). "Identification and sequence analysis of Escherichia coli purE and purK genes encoding 5'-phosphoribosyl-5-amino-4-imidazole carboxylase for de novo purine biosynthesis." J Bacteriol 171:198-204. Pubmed: 2644189