Vitamin B12-binding protein (P37028)

Identification
Name:Vitamin B12-binding protein
Synonyms:Not Available
Gene Name:btuF
Enzyme Class:Not Available
Biological Properties
General Function:Involved in binding
Specific Function:Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC
Cellular Location:Periplasm
SMPDB Pathways:
  • adenosylcobalamin salvage from cobinamide PW001884
KEGG Pathways:
SMPDB Reactions:
1.0Cobinamide+1.0Thumb+1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0Cobinamide+1.0Thumb
1.0Cobinamide + 1.0Adenosine triphosphate + 1.0Water + 1.0Cobinamide → 1.0Adenosine diphosphate + 1.0Phosphate + 1.0Hydrogen ion + 1.0Cobinamide + 1.0ADP
ReactionCard
1.0Cyanocobalamin+1.0Thumb+1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0Cyanocobalamin+1.0Thumb
1.0Cyanocobalamin + 1.0Adenosine triphosphate + 1.0Water + 1.0Cyanocobalamin → 1.0Adenosine diphosphate + 1.0Phosphate + 1.0Hydrogen ion + 1.0Cyanocobalamin + 1.0ADP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Cob(I)alamin → 1.0ADP + 1.0Cob(I)alamin + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Adenosylcobalamin → 1.0Adenosylcobalamin + 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Cobinamide → 1.0ADP + 1.0Cobinamide + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB02086AdenosylcobalaminMetaboCard
ECMDB01341ADPMetaboCard
ECMDB04040Cob(I)alaminMetaboCard
ECMDB06902CobinamideMetaboCard
ECMDB21386CyanocobalaminMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
cation transmembrane transporter activity
di-, tri-valent inorganic cation transmembrane transporter activity
inorganic cation transmembrane transporter activity
ion transmembrane transporter activity
iron ion transmembrane transporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
cation transport
establishment of localization
high-affinity iron ion transport
ion transport
iron ion transmembrane transport
iron ion transport
metal ion transport
transition metal ion transport
transport
Gene Properties
Blattner:b0158
Gene OrientationCounterclockwise
Centisome Percentage:3.83
Left Sequence End177662
Right Sequence End178462
Gene Sequence:
>801 bp
ATGGCTAAGTCACTGTTCAGGGCGCTGGTCGCCCTGTCTTTTCTTGCGCCACTGTGGCTC
AACGCCGCGCCGCGCGTCATCACGCTTTCTCCCGCCAACACTGAACTTGCCTTTGCCGCC
GGGATCACGCCGGTTGGGGTCAGCAGCTATTCCGACTATCCTCCACAAGCGCAAAAGATT
GAGCAGGTTTCCACCTGGCAGGGGATGAATCTGGAACGCATTGTCGCGCTGAAACCCGAT
CTGGTGATTGCCTGGCGTGGAGGTAATGCCGAGCGGCAGGTTGACCAGCTGGCTTCGCTG
GGAATAAAAGTGATGTGGGTCGATGCGACAAGCATTGAACAAATTGCCAATGCGTTACGT
CAACTGGCCCCCTGGAGTCCGCAACCAGACAAGGCCGAACAAGCCGCGCAATCCCTGCTG
GATCAGTACGCGCAATTGAAAGCGCAATATGCTGATAAACCTAAAAAACGTGTTTTTCTG
CAATTCGGCATTAATCCGCCATTTACCAGTGGAAAAGAGTCGATTCAGAACCAGGTACTC
GAAGTTTGTGGCGGAGAAAACATCTTTAAAGACAGCCGGGTTCCCTGGCCGCAAGTTAGC
CGCGAACAGGTGTTAGCACGCTCGCCACAGGCGATTGTCATTACAGGCGGACCGGACCAA
ATTCCTAAAATCAAACAATACTGGGGTGAACAGCTCAAAATTCCCGTTATTCCTCTCACG
AGTGACTGGTTTGAACGTGCAAGCCCACGTATTATCCTCGCTGCACAACAGCTCTGTAAT
GCGCTTTCACAGGTAGATTAG
Protein Properties
Pfam Domain Function:
Protein Residues:266
Protein Molecular Weight:29367
Protein Theoretical pI:9
PDB File:1N2Z
Signaling Regions:
  • 1-22
Transmembrane Regions:
  • None
Protein Sequence:
>Vitamin B12-binding protein
MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITPVGVSSYSDYPPQAQKI
EQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGIKVMWVDATSIEQIANALR
QLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFLQFGINPPFTSGKESIQNQVL
EVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGPDQIPKIKQYWGEQLKIPVIPLT
SDWFERASPRIILAAQQLCNALSQVD
References
External Links:
ResourceLink
Uniprot ID:P37028
Uniprot Name:BTUF_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674362
PDB ID:1N2Z
Ecogene ID:EG12334
Ecocyc:EG12334
ColiBase:b0158
Kegg Gene:b0158
EchoBASE ID:EB2238
CCDB:BTUF_ECOLI
BacMap:16128151
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Borths, E. L., Locher, K. P., Lee, A. T., Rees, D. C. (2002). "The structure of Escherichia coli BtuF and binding to its cognate ATP binding cassette transporter." Proc Natl Acad Sci U S A 99:16642-16647. Pubmed: 12475936
  • Cadieux, N., Bradbeer, C., Reeger-Schneider, E., Koster, W., Mohanty, A. K., Wiener, M. C., Kadner, R. J. (2002). "Identification of the periplasmic cobalamin-binding protein BtuF of Escherichia coli." J Bacteriol 184:706-717. Pubmed: 11790740
  • Fujita, N., Mori, H., Yura, T., Ishihama, A. (1994). "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Nucleic Acids Res 22:1637-1639. Pubmed: 8202364
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Karpowich, N. K., Huang, H. H., Smith, P. C., Hunt, J. F. (2003). "Crystal structures of the BtuF periplasmic-binding protein for vitamin B12 suggest a functionally important reduction in protein mobility upon ligand binding." J Biol Chem 278:8429-8434. Pubmed: 12468528