Lipopolysaccharide core heptose(II) kinase rfaY (P27240)

Identification
Name:Lipopolysaccharide core heptose(II) kinase rfaY
Synonyms:Not Available
Gene Name:rfaY
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transferase activity, transferring phosphorus-containing groups
Specific Function:Catalyzes the phosphorylation of heptose(II) of the outer membrane lipopolysaccharide core
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Lipopolysaccharide biosynthesis ec00540
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0Thumb
1.0glucosyl-(heptosyl)3-Kdo2-lipid A-phosphate + 1.0Adenosine triphosphate → 1.0Adenosine diphosphate + 1.0Hydrogen ion + 1.0glucosyl-(heptosyl)3-Kdo2-lipid A-bisphosphate + 1.0ADP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glucosyl-heptosyl3-KDO2-lipid A-phosphate + 1.0Adenosine triphosphate → 1.0Hydrogen ion + 1.0Glucosyl-heptosyl3-KDO2-lipid A-bisphosphate + 1.0ADP
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Heptosyl-phospho-heptosyl-heptosyl-kdo2-lipidA → 1.0ADP + 1.0Hydrogen ion + 1.0Phospho-heptosyl-phospho-heptosyl-heptosyl-kdo2-lipidA
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB24223glucosyl-(heptosyl)3-Kdo2-lipid A-bisphosphateMetaboCard
ECMDB24222glucosyl-(heptosyl)3-Kdo2-lipid A-phosphateMetaboCard
ECMDB20531Glucosyl-heptosyl3-KDO2-lipid A-bisphosphateMetaboCard
ECMDB20530Glucosyl-heptosyl3-KDO2-lipid A-phosphateMetaboCard
ECMDB21342Heptosyl-phospho-heptosyl-heptosyl-kdo2-lipidAMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21351Phospho-heptosyl-phospho-heptosyl-heptosyl-kdo2-lipidAMetaboCard
GO Classification:
Process
carbohydrate metabolic process
lipopolysaccharide core region biosynthetic process
metabolic process
oligosaccharide biosynthetic process
oligosaccharide metabolic process
primary metabolic process
Gene Properties
Blattner:b3625
Gene OrientationCounterclockwise
Centisome Percentage:81.87
Left Sequence End3798290
Right Sequence End3798988
Gene Sequence:
>699 bp
ATGCAAAAAAACGCTGCGCATACTTATGCCATTTCCAGCTTGTTGGTGCTTTCACTAACC
GGCTGCGCCTGGATACCCTCCACGCCGCTGGTGCAGGGGGCGACCAGTGCACAACCGGTT
CCCGGTCCGACGCCCGTCGCCAACGGTTCTATTTTCCAGTCTGCTCAGCCGATTAACTAT
GGCTATCAACCGCTGTTTGAAGATCGTCGACCACGCAATATTGGCGATACGCTGACCATC
GTGTTGCAGGAGAACGTCAGCGCCAGCAAAAGCTCCTCTGCGAATGCCAGCCGTGACGGT
AAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCT
CGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCC
AGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAAC
CTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTC
TCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTG
GCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGC
TGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:232
Protein Molecular Weight:27461
Protein Theoretical pI:10
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Lipopolysaccharide core heptose(II) kinase rfaY
MIQKSKIKDLVVFTDENNSKYLNVLNDFLSYNINIIKVFRSIDDTKVMLIDTDYGKLILK
VFSPKVKRNERFFKSLLKGDYYERLFEQTQKVRNEGLNTLNDFYLLAERKTLRFVHTYIM
IIEYIDGIELCDMPDIDDALKNKIQQSINALHQHGMVSGDPHRGNFIIKNGEVRIIDLSG
KRASAQRKAKDRIDLERHYGIKNEIRDLGYYLLVYRKKMRNFMRRLKGKPAR
References
External Links:
ResourceLink
Uniprot ID:P27240
Uniprot Name:RFAY_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674831
Ecogene ID:EG11425
Ecocyc:EG11425
ColiBase:b3625
Kegg Gene:b3625
EchoBASE ID:EB1395
CCDB:RFAY_ECOLI
BacMap:16131496
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Klena, J. D., Pradel, E., Schnaitman, C. A. (1992). "Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium." J Bacteriol 174:4746-4752. Pubmed: 1624462
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620