Thiosulfate sulfurtransferase PspE (P23857)

Identification
Name:Thiosulfate sulfurtransferase PspE
Synonyms:
  • TST
  • Phage shock protein E
Gene Name:pspE
Enzyme Class:
Biological Properties
General Function:Inorganic ion transport and metabolism
Specific Function:The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspE catalyzes the sulfur-transfer reaction from thiosulfate to cyanide, to form sulfite and thiocyanate. Also able to use dithiol (dithiothreitol) as an alternate sulfur acceptor. Also possesses a very low mercaptopyruvate sulfurtransferase activity
Cellular Location:Periplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Thiosulfate + 1.0Cyanide ↔ 1.0Sulfite + 1.0Thiocyanate
ReactionCard
SMPDB Reactions:
1.0Cyanide+1.0Thiosulfate+1.0Thumb+1.0Thumb → 1.0Thiocyanate+1.0Sulfite+1.0Thumb+1.0Thumb+1.0Thumb
1.0Cyanide + 1.0Thiosulfate + 1.0Cyanide + 1.0Thiosulfate → 1.0Thiocyanate + 1.0Sulfite + 1.0Hydrogen ion + 1.0Thiocyanate + 1.0Sulfite
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+2.0Thumb
1.0Hydrogen cyanide + 1.0Thiosulfate → 1.0Thiocyanate + 1.0Sulfite + 2.0Hydrogen ion
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Hydrogen cyanide + 1.0Thiosulfate → 1.0Hydrogen ion + 1.0Sulfite + 1.0Thiocyanate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB02084CyanideMetaboCard
ECMDB21231Hydrogen cyanideMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00240SulfiteMetaboCard
ECMDB21289ThiocyanateMetaboCard
ECMDB00257ThiosulfateMetaboCard
GO Classification:Not Available
Gene Properties
Blattner:b1308
Gene OrientationClockwise
Centisome Percentage:29.48
Left Sequence End1367713
Right Sequence End1368027
Gene Sequence:
>315 bp
ATGTTTAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGCCCGTTTTCGCCGCT
GAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAAGAGCACGTTCAGGGGGCC
ATCAATATTCCCCTGAAGGAAGTGAAAGAGCGCATTGCCACCGCCGTTCCGGATAAAAAC
GACACCGTGAAAGTGTATTGCAATGCCGGACGCCAGTCAGGGCAAGCAAAAGAGATCCTT
AGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCG
AAGGTCAAAGGTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:104
Protein Molecular Weight:11475
Protein Theoretical pI:8
Signaling Regions:
  • 1-19
Transmembrane Regions:
  • None
Protein Sequence:
>Thiosulfate sulfurtransferase PspE
MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIPLKEVKERIATAVPDKN
DTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKVKG
References
External Links:
ResourceLink
Uniprot ID:P23857
Uniprot Name:PSPE_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742138
Ecogene ID:EG10780
Ecocyc:EG10780
ColiBase:b1308
Kegg Gene:b1308
EchoBASE ID:EB0773
CCDB:PSPE_ECOLI
BacMap:16129269
General Reference:
  • Adams, H., Teertstra, W., Koster, M., Tommassen, J. (2002). "PspE (phage-shock protein E) of Escherichia coli is a rhodanese." FEBS Lett 518:173-176. Pubmed: 11997041
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Brissette, J. L., Weiner, L., Ripmaster, T. L., Model, P. (1991). "Characterization and sequence of the Escherichia coli stress-induced psp operon." J Mol Biol 220:35-48. Pubmed: 1712397
  • Cheng, H., Donahue, J. L., Battle, S. E., Ray, W. K., Larson, T. J. (2008). "Biochemical and Genetic Characterization of PspE and GlpE, Two Single-domain Sulfurtransferases of Escherichia coli." Open Microbiol J 2:18-28. Pubmed: 19088907
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Li, H., Yang, F., Kang, X., Xia, B., Jin, C. (2008). "Solution structures and backbone dynamics of Escherichia coli rhodanese PspE in its sulfur-free and persulfide-intermediate forms: implications for the catalytic mechanism of rhodanese." Biochemistry 47:4377-4385. Pubmed: 18355042
  • Wasinger, V. C., Humphery-Smith, I. (1998). "Small genes/gene-products in Escherichia coli K-12." FEMS Microbiol Lett 169:375-382. Pubmed: 9868784