4-hydroxybenzoate octaprenyltransferase (P0AGK1)

Identification
Name:4-hydroxybenzoate octaprenyltransferase
Synonyms:
  • 4-HB polyprenyltransferase
Gene Name:ubiA
Enzyme Class:
Biological Properties
General Function:Involved in prenyltransferase activity
Specific Function:Synthesis of 3-octaprenyl-4-hydroxybenzoate
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
  • Secondary Metabolites: Ubiquinol biosynthesis PW000981
  • Secondary Metabolites: Ubiquinol biosynthesis 2 PW002036
KEGG Pathways:
  • Metabolic pathways eco01100
  • Ubiquinone and other terpenoid-quinone biosynthesis ec00130
KEGG Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.04-Hydroxybenzoic acid + 1.0Octaprenyl diphosphate → 1.03-Octaprenyl-4-hydroxybenzoate + 1.0Pyrophosphate
ReactionCard
1.0all-trans-Polyprenyl diphosphate+1.0Thumb+1.0Thumb ↔ 1.04-Hydroxy-3-polyprenylbenzoate+1.0Thumb+1.0Thumb
1.0all-trans-Polyprenyl diphosphate + 1.04-Hydroxybenzoic acid + 1.0Geranyl-PP ↔ 1.04-Hydroxy-3-polyprenylbenzoate + 1.0Pyrophosphate + 1.04-Hydroxy-3-polyprenylbenzoate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Octaprenyl diphosphate + 1.04-Hydroxybenzoic acid ↔ 1.03-Octaprenyl-4-hydroxybenzoate + 1.0Pyrophosphate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Octaprenyl diphosphate+1.0Thumb → 1.0Pyrophosphate+1.0Thumb
1.04-Hydroxybenzoic acid + 1.0Octaprenyl diphosphate + 1.0Octaprenyl diphosphate → 1.0Pyrophosphate + 1.03-Octaprenyl-4-hydroxybenzoate
ReactionCard
EcoCyc Reactions:
1.0all-trans-octaprenyl diphosphate+1.0Thumb → 1.0Thumb+1.0Thumb
1.0all-trans-octaprenyl diphosphate + 1.04-Hydroxybenzoic acid → 1.0Pyrophosphate + 1.03-Octaprenyl-4-hydroxybenzoate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb
1.04-Hydroxybenzoic acid + 1.0Octaprenyl diphosphate → 1.03-Octaprenyl-4-hydroxybenzoate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB211693-Octaprenyl-4-hydroxybenzoateMetaboCard
ECMDB237564-Hydroxy-3-polyprenylbenzoateMetaboCard
ECMDB005004-Hydroxybenzoic acidMetaboCard
ECMDB21529Farnesylfarnesylgeranyl-PPMetaboCard
ECMDB01285Geranyl-PPMetaboCard
ECMDB01094Octaprenyl diphosphateMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane part
Function
catalytic activity
prenyltransferase activity
transferase activity
transferase activity, transferring alkyl or aryl (other than methyl) groups
Process
biosynthetic process
metabolic process
Gene Properties
Blattner:b4040
Gene OrientationClockwise
Centisome Percentage:91.62
Left Sequence End4251039
Right Sequence End4251911
Gene Sequence:
>873 bp
ATGTGTACAACATGCGGTTGCGGTGAAGGCAACCTGTATATCGAGGGTGATGAACATAAC
CCTCATTCCGCGTTTCGTAGCGCGCCATTTGCCCCGGCGGCACGCCCGAAGATGAAAATC
ACCGGCATTAAAGCGCCTGAATTTACCCCCAGCCAGACTGAAGAAGGCGACCTGCATTAC
GGTCATGGCGAAGCGGGCACTCACGCACCGGGCATGAGCCAGCGTCGGATGCTGGAAGTC
GAAATTGACGTGCTGGACAAAAATAACCGTCTGGCTGAACGCAACCGCGCGCGCTTTGCT
GCCCGCAAGCAACTGGTGCTCAACCTGGTTTCCAGCCCTGGTTCCGGTAAAACCACCCTG
CTGACGGAAACCCTAATGCGCCTGAAAGACAGCGTTCCGTGCGCAGTTATTGAAGGCGAC
CAGCAAACCGTGAACGATGCCGCACGCATTCGCGCTACCGGCACACCAGCGATTCAGGTG
AACACCGGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTG
CCACTGGACGATAACGGTATTCTGTTTATCGAAAACGTTGGCAACCTCGTATGCCCGGCC
AGCTTCGATCTCGGTGAAAAACACAAAGTGGCGGTGCTTTCCGTTACCGAAGGTGAAGAC
AAACCACTGAAATATCCGCATATGTTTGCCGCCGCCTCGCTGATGCTGCTCAACAAAGTT
GACCTGTTGCCGTATCTCAACTTTGACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTC
AATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGG
CTGAACTGGCTGGAGACACAGCGATGTGCATAG
Protein Properties
Pfam Domain Function:
Protein Residues:290
Protein Molecular Weight:32511
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • 23-43
  • 46-66
  • 100-120
  • 141-161
  • 163-183
  • 213-233
  • 234-254
  • 268-288
Protein Sequence:
>4-hydroxybenzoate octaprenyltransferase
MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVPQLWILAVFVAGVWLMR
AAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVVLVLISFLLVLTLNTMTIL
LSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAAVSESVPLSCWLMFLANILWA
VAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGILQIGVLALMAIIGELNGLGWGYY
WSILVAGALFVYQQKLIANREREACFKAFMNNNYVGLVLFLGLAMSYWHF
References
External Links:
ResourceLink
Uniprot ID:P0AGK1
Uniprot Name:UBIA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675548
Ecogene ID:EG11370
Ecocyc:EG11370
ColiBase:b4040
Kegg Gene:b4040
EchoBASE ID:EB1344
CCDB:UBIA_ECOLI
BacMap:16131866
General Reference:
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Melzer, M., Heide, L. (1994). "Characterization of polyprenyldiphosphate: 4-hydroxybenzoate polyprenyltransferase from Escherichia coli." Biochim Biophys Acta 1212:93-102. Pubmed: 8155731
  • Nichols, B. P., Green, J. M. (1992). "Cloning and sequencing of Escherichia coli ubiC and purification of chorismate lyase." J Bacteriol 174:5309-5316. Pubmed: 1644758
  • Nishimura, K., Nakahigashi, K., Inokuchi, H. (1992). "Location of the ubiA gene on the physical map of Escherichia coli." J Bacteriol 174:5762. Pubmed: 1512213
  • Siebert, M., Bechthold, A., Melzer, M., May, U., Berger, U., Schroder, G., Schroder, J., Severin, K., Heide, L. (1992). "Ubiquinone biosynthesis. Cloning of the genes coding for chorismate pyruvate-lyase and 4-hydroxybenzoate octaprenyl transferase from Escherichia coli." FEBS Lett 307:347-350. Pubmed: 1644192
  • Suzuki, K., Ueda, M., Yuasa, M., Nakagawa, T., Kawamukai, M., Matsuda, H. (1994). "Evidence that Escherichia coli ubiA product is a functional homolog of yeast COQ2, and the regulation of ubiA gene expression." Biosci Biotechnol Biochem 58:1814-1819. Pubmed: 7765507
  • Wu, G., Williams, H. D., Gibson, F., Poole, R. K. (1993). "Mutants of Escherichia coli affected in respiration: the cloning and nucleotide sequence of ubiA, encoding the membrane-bound p-hydroxybenzoate:octaprenyltransferase." J Gen Microbiol 139:1795-1805. Pubmed: 8409922