Spermidine/putrescine-binding periplasmic protein (P0AFK9)

Identification
Name:Spermidine/putrescine-binding periplasmic protein
Synonyms:
  • SPBP
Gene Name:potD
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transporter activity
Specific Function:Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein
Cellular Location:Periplasm
SMPDB Pathways:
KEGG Pathways:
Transports:
Transport References:Not Available
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Putrescine → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0Putrescine
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Spermidine → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0Spermidine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB01414PutrescineMetaboCard
ECMDB01257SpermidineMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
outer membrane-bounded periplasmic space
periplasmic space
Function
transporter activity
Process
establishment of localization
transport
Gene Properties
Blattner:b1123
Gene OrientationCounterclockwise
Centisome Percentage:25.45
Left Sequence End1181006
Right Sequence End1182052
Gene Sequence:
>1047 bp
ATGAAAAAATGGTCACGCCACCTGCTCGCGGCGGGTGCTCTGGCACTGGGCATGAGCGCC
GCTCACGCCGATGACAACAACACGCTGTATTTCTACAACTGGACCGAGTACGTGCCGCCA
GGACTGCTTGAACAGTTCACCAAAGAAACCGGTATTAAGGTTATCTATTCGACTTACGAG
TCGAACGAAACCATGTACGCCAAGCTGAAAACATACAAAGACGGTGCCTATGATCTGGTG
GTTCCTTCAACCTATTACGTTGATAAAATGCGTAAAGAAGGGATGATCCAGAAGATCGAC
AAGTCGAAGTTAACAAACTTCAGCAATCTCGATCCAGACATGCTCAACAAGCCTTTTGAC
CCGAATAACGACTACTCCATTCCGTATATCTGGGGTGCGACGGCGATTGGTGTTAACGGT
GATGCGGTGGATCCGAAATCTGTCACCAGCTGGGCCGATCTGTGGAAGCCAGAGTACAAA
GGCAGCCTGCTGTTGACCGACGATGCCCGTGAAGTGTTCCAGATGGCGCTGCGTAAGCTG
GGCTACTCCGGTAACACCACCGATCCGAAAGAGATTGAAGCTGCATATAACGAGCTGAAA
AAACTGATGCCAAACGTGGCAGCCTTTAACTCCGATAACCCGGCGAACCCGTACATGGAA
GGCGAAGTTAACCTCGGCATGATCTGGAACGGTTCTGCTTTCGTTGCACGCCAGGCGGGT
ACGCCAATTGACGTGGTGTGGCCGAAAGAAGGCGGCATTTTCTGGATGGACAGCCTGGCG
ATCCCGGCAAATGCCAAAAACAAAGAAGGCGCGCTGAAATTGATCAACTTCCTGCTGCGC
CCGGATGTGGCAAAACAGGTTGCTGAAACTATCGGTTATCCAACGCCAAACCTTGCGGCG
CGTAAGCTGTTAAGTCCAGAAGTGGCGAACGATAAAACACTCTACCCGGATGCTGAAACC
ATTAAAAATGGCGAATGGCAGAATGACGTTGGCGCAGCCAGCAGCATTTATGAAGAGTAT
TATCAGAAGCTGAAAGCAGGACGTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:348
Protein Molecular Weight:38867
Protein Theoretical pI:5
PDB File:1POT
Signaling Regions:
  • 1-23
Transmembrane Regions:
  • None
Protein Sequence:
>Spermidine/putrescine-binding periplasmic protein
MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLEQFTKETGIKVIYSTYE
SNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKSKLTNFSNLDPDMLNKPFD
PNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYKGSLLLTDDAREVFQMALRKL
GYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPYMEGEVNLGMIWNGSAFVARQAG
TPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLINFLLRPDVAKQVAETIGYPTPNLAA
RKLLSPEVANDKTLYPDAETIKNGEWQNDVGAASSIYEEYYQKLKAGR
References
External Links:
ResourceLink
Uniprot ID:P0AFK9
Uniprot Name:POTD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651550
PDB ID:1POT
Ecogene ID:EG10752
Ecocyc:EG10752
ColiBase:b1123
Kegg Gene:b1123
EchoBASE ID:EB0745
CCDB:POTD_ECOLI
BacMap:16129086
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Furuchi, T., Kashiwagi, K., Kobayashi, H., Igarashi, K. (1991). "Characteristics of the gene for a spermidine and putrescine transport system that maps at 15 min on the Escherichia coli chromosome." J Biol Chem 266:20928-20933. Pubmed: 1939142
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Matsuo, Y., Nishikawa, K. (1994). "Prediction of the structural similarity between spermidine/putrescine-binding protein and maltose-binding protein." FEBS Lett 345:23-26. Pubmed: 8194593
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Sugiyama, S., Matsuo, Y., Maenaka, K., Vassylyev, D. G., Matsushima, M., Kashiwagi, K., Igarashi, K., Morikawa, K. (1996). "The 1.8-A X-ray structure of the Escherichia coli PotD protein complexed with spermidine and the mechanism of polyamine binding." Protein Sci 5:1984-1990. Pubmed: 8897598
  • Sugiyama, S., Vassylyev, D. G., Matsushima, M., Kashiwagi, K., Igarashi, K., Morikawa, K. (1996). "Crystal structure of PotD, the primary receptor of the polyamine transport system in Escherichia coli." J Biol Chem 271:9519-9525. Pubmed: 8621624