Arabinose import ATP-binding protein AraG (P0AAF3)

Identification
Name:Arabinose import ATP-binding protein AraG
Synonyms:Not Available
Gene Name:araG
Enzyme Class:
Biological Properties
General Function:Involved in nucleotide binding
Specific Function:Part of the ABC transporter complex AraFGH involved in arabinose import. Responsible for energy coupling to the transport system (Probable)
Cellular Location:Cell inner membrane; Peripheral membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0L-Arabinose → 1.0ADP + 1.0L-Arabinose + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB00646L-ArabinoseMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
arabinose transmembrane transporter activity
ATP binding
ATPase activity
binding
carbohydrate transmembrane transporter activity
catalytic activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
L-arabinose transmembrane transporter activity
L-arabinose-importing ATPase activity
monosaccharide transmembrane transporter activity
nucleoside binding
nucleoside-triphosphatase activity
nucleotide binding
pentose transmembrane transporter activity
purine nucleoside binding
pyrophosphatase activity
substrate-specific transmembrane transporter activity
sugar transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
arabinose transport
carbohydrate transport
establishment of localization
L-arabinose transport
monosaccharide transport
pentose transport
transport
Gene Properties
Blattner:b1900
Gene OrientationCounterclockwise
Centisome Percentage:42.71
Left Sequence End1981579
Right Sequence End1983093
Gene Sequence:
>1515 bp
ATGCAACAGTCTACCCCGTATCTCTCATTTCGCGGCATCGGTAAAACGTTTCCCGGCGTT
AAGGCGCTGACGGATATTAGTTTTGACTGCTATGCCGGTCAGGTTCATGCGTTGATGGGT
GAAAATGGCGCAGGAAAATCAACTCTCTTAAAAATCCTCAGCGGCAACTATGCGCCAACC
ACGGGTTCTGTAGTGATTAATGGGCAGGAAATGTCCTTTTCCGACACGACCGCAGCACTT
AACGCGGGCGTGGCGATTATTTACCAGGAACTGCATCTCGTGCCGGAAATGACCGTCGCG
GAAAACATCTATCTCGGCCAGCTGCCGCATAAAGGCGGCATTGTGAATCGCTCATTGCTG
AATTATGAGGCGGGTTTACAACTTAAACATCTTGGTATGGATATTGACCCGGACACGCCG
CTGAAATATCTCTCCATTGGTCAGTGGCAGATGGTTGAAATCGCCAAAGCGCTGGCGCGT
AACGCCAAAATTATCGCCTTTGATGAGCCAACCAGCTCCCTCTCTGCCCGTGAAATCGAC
AATCTTTTCCGCGTTATTCGTGAACTGCGAAAAGAGGGGCGGGTAATCTTATACGTTTCT
CACCGTATGGAAGAAATATTTGCCCTCAGCGATGCCATTACTGTCTTTAAAGATGGACGT
TATGTCAAAACCTTTACCGATATGCAGCAGGTTGACCACGACGCGCTGGTGCAGGCGATG
GTCGGGCGCGACATTGGCGATATCTACGGCTGGCAACCGCGTAGTTATGGCGAGGAGCGC
CTACGTCTTGATGCTGTGAAAGCACCAGGCGTGCGTACGCCAATAAGTCTGGCGGTTCGC
AGTGGTGAAATTGTTGGGCTGTTTGGTCTGGTAGGGGCGGGGCGTAGCGAATTAATGAAA
GGCATGTTTGGCGGGACGCAAATCACCGCCGGTCAGGTTTATATCGACCAACAGCCGATC
GATATTCGTAAACCGAGCCACGCCATTGCCGCAGGCATGATGCTCTGCCCGGAAGATCGC
AAAGCGGAAGGCATTATTCCCGTGCACTCCGTTCGCGACAATATCAACATCAGTGCCAGA
CGTAAACATGTGCTCGGCGGTTGTGTAATCAACAACGGTTGGGAAGAAAACAATGCCGAT
CACCACATTCGTTCGCTCAACATCAAAACGCCGGGCGCGGAGCAACTGATCATGAATCTC
TCAGGCGGAAATCAGCAAAAAGCCATTCTGGGCCGCTGGTTATCGGAAGAGATGAAGGTC
ATTTTGCTGGATGAACCTACGCGCGGCATTGATGTTGGCGCTAAGCACGAAATATATAAC
GTAATTTATGCGCTGGCGGCGCAGGGCGTGGCGGTGCTGTTTGCCTCCAGCGACTTACCT
GAAGTCCTCGGCGTTGCCGACCGGATTGTGGTGATGCGGGAAGGTGAAATCGCCGGTGAA
TTGTTACACGAGCAGGCAGATGAGCGTCAGGCACTGAGCCTTGCGATGCCTAAAGTCAGC
CAGGCTGTTGCCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:504
Protein Molecular Weight:55018
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Arabinose import ATP-binding protein AraG
MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGAGKSTLLKILSGNYAPT
TGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAENIYLGQLPHKGGIVNRSLL
NYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALARNAKIIAFDEPTSSLSAREID
NLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKDGRYVKTFTDMQQVDHDALVQAM
VGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPISLAVRSGEIVGLFGLVGAGRSELMK
GMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMMLCPEDRKAEGIIPVHSVRDNINISAR
RKHVLGGCVINNGWEENNADHHIRSLNIKTPGAEQLIMNLSGGNQQKAILGRWLSEEMKV
ILLDEPTRGIDVGAKHEIYNVIYALAAQGVAVLFASSDLPEVLGVADRIVVMREGEIAGE
LLHEQADERQALSLAMPKVSQAVA
References
External Links:
ResourceLink
Uniprot ID:P0AAF3
Uniprot Name:ARAG_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1736558
Ecogene ID:EG10058
Ecocyc:EG10058
ColiBase:b1900
Kegg Gene:b1900
EchoBASE ID:EB0056
CCDB:ARAG_ECOLI
BacMap:16129850
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Horazdovsky, B. F., Hogg, R. W. (1989). "Genetic reconstitution of the high-affinity L-arabinose transport system." J Bacteriol 171:3053-3059. Pubmed: 2656640
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Kolodrubetz, D., Schleif, R. (1981). "L-arabinose transport systems in Escherichia coli K-12." J Bacteriol 148:472-479. Pubmed: 7028715
  • Scripture, J. B., Voelker, C., Miller, S., O'Donnell, R. T., Polgar, L., Rade, J., Horazdovsky, B. F., Hogg, R. W. (1987). "High-affinity L-arabinose transport operon. Nucleotide sequence and analysis of gene products." J Mol Biol 197:37-46. Pubmed: 2445996