Galactokinase (P0A6T3)

Identification
Name:Galactokinase
Synonyms:
  • Galactose kinase
Gene Name:galK
Enzyme Class:
Biological Properties
General Function:Involved in galactokinase activity
Specific Function:ATP + D-galactose = ADP + alpha-D-galactose 1- phosphate
Cellular Location:Cytoplasm (Potential)
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0D-Galactose + 1.0Alpha-D-Galactose ↔ 1.0ADP + 1.0Galactose 1-phosphate + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0D-Galactose ↔ 1.0ADP + 1.0Galactose 1-phosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0D-Galactose + 1.0Adenosine triphosphate → 1.0Hydrogen ion + 1.0Galactose 1-phosphate + 1.0ADP
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0Galactose 1-phosphate+1.0Thumb+1.0Thumb
1.0Alpha-D-Galactose + 1.0Adenosine triphosphate → 1.0Adenosine diphosphate + 1.0Hydrogen ion + 1.0Galactose 1-phosphate + 1.0ADP + 1.0Galactose 1-phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0D-Galactose + 1.0Alpha-D-Galactose ↔ 1.0ADP + 1.0Galactose 1-phosphate + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0D-Galactose + 1.0Adenosine triphosphate → 1.0Hydrogen ion + 1.0Galactose 1-phosphate + 1.0ADP
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0D-Galactose → 1.0ADP + 1.0Galactose 1-phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB04012Alpha-D-GalactoseMetaboCard
ECMDB00143D-GalactoseMetaboCard
ECMDB04011Galactose 1-phosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
galactokinase activity
kinase activity
nucleoside binding
phosphotransferase activity, alcohol group as acceptor
purine nucleoside binding
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
alcohol metabolic process
carbohydrate phosphorylation
cellular metabolic process
galactose metabolic process
hexose metabolic process
metabolic process
monosaccharide metabolic process
phosphate metabolic process
phosphorus metabolic process
phosphorylation
small molecule metabolic process
Gene Properties
Blattner:b0757
Gene OrientationCounterclockwise
Centisome Percentage:16.99
Left Sequence End788054
Right Sequence End789202
Gene Sequence:
>1149 bp
TTGATTAAGTCAGCGCTATTGGTTCTGGAAGACGGAACCCAGTTTCACGGTCGGGCCATA
GGGGCAACAGGTTCGGCGGTTGGGGAAGTCGTTTTCAATACTTCAATGACCGGTTATCAA
GAAATCCTCACTGATCCTTCCTATTCTCGTCAAATCGTTACTCTTACTTATCCCCATATT
GGCAATGTCGGCACCAATGACGCCGATGAAGAATCTTCTCAGGTACATGCACAAGGTCTG
GTGATTCGCGACCTGCCGCTGATTGCCAGCAACTTCCGTAATACCGAAGACCTCTCTTCT
TACCTGAAACGCCATAACATCGTGGCGATTGCCGATATCGATACCCGTAAGCTGACGCGT
TTACTGCGCGAGAAAGGCGCACAGAATGGCTGCATTATCGCGGGCGATAACCCGGATGCG
GCGCTGGCGTTAGAAAAAGCCCGCGCGTTCCCAGGTCTGAATGGCATGGATCTGGCAAAA
GAAGTGACCACCGCAGAAGCCTATAGCTGGACACAAGGGAGCTGGACGTTGACCGGTGGC
CTGCCAGAAGCGAAAAAAGAAGACGAGCTGCCGTTCCACGTCGTGGCTTATGATTTTGGT
GCCAAGCGCAACATCCTGCGGATGCTGGTGGATAGAGGCTGTCGCCTGACCATCGTTCCG
GCGCAAACTTCTGCGGAAGATGTGCTGAAAATGAATCCAGACGGCATCTTCCTCTCCAAC
GGTCCTGGCGACCCGGCCCCGTGCGATTACGCCATTACCGCCATCCAGAAATTCCTCGAA
ACCGATATTCCGGTATTCGGCATCTGTCTCGGTCATCAGCTGCTGGCGCTGGCGAGCGGT
GCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTG
GAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACA
TTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATT
CATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCA
CACGACGCCGCGCCGTTGTTCGACCACTTTATCGAGTTAATTGAGCAGTACCGTAAAACC
GCTAAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:382
Protein Molecular Weight:41442
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Galactokinase
MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLPCAIDYQTVISCAPRD
DRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQLRNNSFGGVDMVISGN
VPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAENQFVGCNCGIMDQLISAL
GKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSEYNTRREQCETGARFFQQP
ALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASALEQGDLKRMGELMAESHAS
MRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCIVALIPEELVPAVQQAVAEQY
EAKTGIKETFYVCKPSQGAGQC
References
External Links:
ResourceLink
Uniprot ID:P0A6T3
Uniprot Name:GAL1_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321914
Ecogene ID:EG10363
Ecocyc:EG10363
ColiBase:b0757
Kegg Gene:b0757
EchoBASE ID:EB0358
CCDB:GAL1_ECOLI
BacMap:16128725
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bouffard, G. G., Rudd, K. E., Adhya, S. L. (1994). "Dependence of lactose metabolism upon mutarotase encoded in the gal operon in Escherichia coli." J Mol Biol 244:269-278. Pubmed: 7966338
  • Debouck, C., Riccio, A., Schumperli, D., McKenney, K., Jeffers, J., Hughes, C., Rosenberg, M., Heusterspreute, M., Brunel, F., Davison, J. (1985). "Structure of the galactokinase gene of Escherichia coli, the last (?) gene of the gal operon." Nucleic Acids Res 13:1841-1853. Pubmed: 3158881
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Schlesinger, D. H., Schell, M. A., Wilson, D. B. (1977). "The NH2-terminal sequences of galactokinase from Escherichia coli and Saccharomyces cerevisiae." FEBS Lett 83:45-47. Pubmed: 200486