Record Information
Version2.0
Creation Date2012-05-31 13:45:10 -0600
Update Date2015-06-03 15:53:44 -0600
Secondary Accession Numbers
  • ECMDB01096
Identification
Name:Carbamoylphosphate
Description:Carbamoyl phosphate is a precursor of both arginine and pyrimidine biosynthesis. It is a labile and potentially toxic intermediate. Carbamoyl phosphate is produced from carbon dioxide, ammonia, and phosphate (from ATP) by the enzyme carbamoyl phosphate synthase. -- Wikipedia
Structure
Thumb
Synonyms:
  • Carbamate monoanhydride with phosphate
  • Carbamate monoanhydride with phosphorate
  • Carbamate monoanhydride with phosphoric acid
  • Carbamic acid monoanhydride with phosphorate
  • Carbamic acid monoanhydride with phosphoric acid
  • Carbamoyl phosphate
  • Carbamoyl phosphoric acid
  • Carbamoyl-P
  • Carbamoyl-phosphate
  • Carbamoyl-phosphoric acid
  • Carbamoylphosphate
  • Carbamoylphosphoric acid
  • Carbamyl phosphate
  • Carbamyl phosphoric acid
  • Carbamyl-phosphate
  • Carbamyl-phosphoric acid
Chemical Formula:CH4NO5P
Weight:Average: 141.0199
Monoisotopic: 140.982708755
InChI Key:FFQKYPRQEYGKAF-UHFFFAOYSA-N
InChI:InChI=1S/CH4NO5P/c2-1(3)7-8(4,5)6/h(H2,2,3)(H2,4,5,6)
CAS number:590-55-6
IUPAC Name:(carbamoyloxy)phosphonic acid
Traditional IUPAC Name:carbamoyl-phosphate
SMILES:NC(=O)OP(O)(O)=O
Chemical Taxonomy
DescriptionThis compound belongs to the class of chemical entities known as organic phosphoric acids and derivatives. These are organic compounds containing phosphoric acid or a derivative thereof.
KingdomChemical entities
Super ClassOrganic compounds
ClassOrganic acids and derivatives
Sub ClassOrganic phosphoric acids and derivatives
Direct ParentOrganic phosphoric acids and derivatives
Alternative Parents
Substituents
  • Organic phosphoric acid derivative
  • Carboximidic acid derivative
  • Organic nitrogen compound
  • Organic oxygen compound
  • Organopnictogen compound
  • Organic oxide
  • Hydrocarbon derivative
  • Organooxygen compound
  • Organonitrogen compound
  • Imine
  • Aliphatic acyclic compound
Molecular FrameworkAliphatic acyclic compounds
External Descriptors
Physical Properties
State:Solid
Charge:-2
Melting point:Not Available
Experimental Properties:
PropertyValueSource
Predicted Properties
PropertyValueSource
Water Solubility15.6 mg/mLALOGPS
logP-1.5ALOGPS
logP-1.2ChemAxon
logS-0.96ALOGPS
pKa (Strongest Acidic)1.1ChemAxon
Physiological Charge-2ChemAxon
Hydrogen Acceptor Count4ChemAxon
Hydrogen Donor Count3ChemAxon
Polar Surface Area109.85 Å2ChemAxon
Rotatable Bond Count2ChemAxon
Refractivity22.48 m3·mol-1ChemAxon
Polarizability9.12 Å3ChemAxon
Number of Rings0ChemAxon
Bioavailability1ChemAxon
Rule of FiveYesChemAxon
Ghose FilterYesChemAxon
Veber's RuleYesChemAxon
MDDR-like RuleYesChemAxon
Biological Properties
Cellular Locations:Cytoplasm
Reactions:
L-Aspartic acid + Carbamoylphosphate <> Ureidosuccinic acid + Hydrogen ion + Phosphate
2 Adenosine triphosphate + L-Glutamine + Water + Hydrogen carbonate >2 ADP + Carbamoylphosphate + L-Glutamate +2 Hydrogen ion + Phosphate
Adenosine triphosphate + Carbon dioxide + Ammonium <> ADP + Carbamoylphosphate +2 Hydrogen ion
Carbamoylphosphate + Ornithine + L-Ornithine <> Citrulline + Hydrogen ion + Phosphate
Adenosine triphosphate + Ammonia + Carbon dioxide <> ADP + Carbamoylphosphate
2 Adenosine triphosphate + L-Glutamine + Hydrogen carbonate + Water <>2 ADP + Phosphate + L-Glutamate + Carbamoylphosphate
Adenosine triphosphate + Carbamic acid <> ADP + Carbamoylphosphate
Carbamoylphosphate + L-Aspartic acid <> Phosphate + Ureidosuccinic acid
Carbamoylphosphate + Ornithine <> Phosphate + Citrulline
L-Aspartic acid + Carbamoylphosphate > Hydrogen ion + Ureidosuccinic acid + Phosphate
Ammonia + Carbon dioxide + Adenosine triphosphate < Hydrogen ion + Carbamoylphosphate + ADP
Adenosine triphosphate + L-Glutamine + Hydrogen carbonate + Water > Hydrogen ion + Carbamoylphosphate + L-Glutamate + Phosphate + ADP
Ornithine + Carbamoylphosphate <> Hydrogen ion + Citrulline + Phosphate
Oxamate + Carbamoylphosphate < Phosphate + Oxalureate
Adenosine triphosphate + Hydrogen carbonate + Ammonia > ADP + Phosphate + Carbamoylphosphate + Hydrogen ion
Adenosine triphosphate + Ammonia + Carbon dioxide > ADP + Carbamoylphosphate
2 Adenosine triphosphate + L-Glutamine + Carbonic acid + Water >2 ADP + Inorganic phosphate + L-Glutamate + Carbamoylphosphate
2 Adenosine triphosphate + L-Glutamine + Carbonic acid + Water >2 ADP + Inorganic phosphate + L-Glutamate + Carbamoylphosphate
Carbamoylphosphate + Ornithine > Inorganic phosphate + Citrulline
Carbamoylphosphate + Ornithine > Inorganic phosphate + Citrulline
Carbamoylphosphate + L-Aspartic acid > Inorganic phosphate + Ureidosuccinic acid
2 Adenosine triphosphate + L-Glutamine + Hydrogen carbonate + Water + Ammonia + Carbamic acid + Carboxyphosphate <>2 ADP + Phosphate + L-Glutamate + Carbamoylphosphate
Ornithine + Carbamoylphosphate + Ornithine > Phosphate + Hydrogen ion + Citrulline
Hydrogen carbonate + Water + L-Glutamine + 2 Adenosine triphosphate >2 Adenosine diphosphate + Phosphate + L-Glutamic acid +2 Hydrogen ion + Carbamoylphosphate +2 ADP + L-Glutamate
Carbamoylphosphate + L-Aspartic acid + L-Aspartic acid > Phosphate + Hydrogen ion + N-carbamoyl-L-aspartate

SMPDB Pathways:
Nitrogen metabolismPW000755 Pw000755Pw000755 greyscalePw000755 simple
Pyrimidine metabolismPW000942 Pw000942Pw000942 greyscalePw000942 simple
allantoin degradation (anaerobic)PW002050 Pw002050Pw002050 greyscalePw002050 simple
arginine metabolismPW000790 Pw000790Pw000790 greyscalePw000790 simple
KEGG Pathways:
EcoCyc Pathways:
Concentrations
Not Available
Spectra
Spectra:
Spectrum TypeDescriptionSplash Key
GC-MSGC-MS Spectrum - GC-MSsplash10-0002-9100000000-6a951539300f7f5b64d6View in MoNA
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 10V, Positivesplash10-0002-9000000000-1e9b8619eed467aca3ccView in MoNA
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 20V, Positivesplash10-0002-9000000000-49d39aaea7b7b239f046View in MoNA
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 40V, Positivesplash10-0005-9000000000-ff8b83b8641d1d23539fView in MoNA
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 10V, Negativesplash10-002b-9100000000-9b7601209f4777504c50View in MoNA
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 20V, Negativesplash10-004i-9000000000-068148ee9b7b7acd6692View in MoNA
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 40V, Negativesplash10-004i-9000000000-2eef12354837d65af0c1View in MoNA
References
References:
  • Chen KF, Lai YY, Sun HS, Tsai SJ: Transcriptional repression of human cad gene by hypoxia inducible factor-1alpha. Nucleic Acids Res. 2005 Sep 9;33(16):5190-8. Print 2005. Pubmed: 16155188
  • Kanehisa, M., Goto, S., Sato, Y., Furumichi, M., Tanabe, M. (2012). "KEGG for integration and interpretation of large-scale molecular data sets." Nucleic Acids Res 40:D109-D114. Pubmed: 22080510
  • Keseler, I. M., Collado-Vides, J., Santos-Zavaleta, A., Peralta-Gil, M., Gama-Castro, S., Muniz-Rascado, L., Bonavides-Martinez, C., Paley, S., Krummenacker, M., Altman, T., Kaipa, P., Spaulding, A., Pacheco, J., Latendresse, M., Fulcher, C., Sarker, M., Shearer, A. G., Mackie, A., Paulsen, I., Gunsalus, R. P., Karp, P. D. (2011). "EcoCyc: a comprehensive database of Escherichia coli biology." Nucleic Acids Res 39:D583-D590. Pubmed: 21097882
  • Schnater JM, Bruder E, Bertschin S, Woodtli T, de Theije C, Pietsch T, Aronson DC, von Schweinitz D, Lamers WH, Kohler ES: Subcutaneous and intrahepatic growth of human hepatoblastoma in immunodeficient mice. J Hepatol. 2006 Sep;45(3):377-86. Epub 2006 May 3. Pubmed: 16780998
  • Sigoillot FD, Kotsis DH, Serre V, Sigoillot SM, Evans DR, Guy HI: Nuclear localization and mitogen-activated protein kinase phosphorylation of the multifunctional protein CAD. J Biol Chem. 2005 Jul 8;280(27):25611-20. Epub 2005 May 12. Pubmed: 15890648
  • Struck J, Uhlein M, Morgenthaler NG, Furst W, Hoflich C, Bahrami S, Bergmann A, Volk HD, Redl H: Release of the mitochondrial enzyme carbamoyl phosphate synthase under septic conditions. Shock. 2005 Jun;23(6):533-8. Pubmed: 15897806
  • van der Werf, M. J., Overkamp, K. M., Muilwijk, B., Coulier, L., Hankemeier, T. (2007). "Microbial metabolomics: toward a platform with full metabolome coverage." Anal Biochem 370:17-25. Pubmed: 17765195
  • Winder, C. L., Dunn, W. B., Schuler, S., Broadhurst, D., Jarvis, R., Stephens, G. M., Goodacre, R. (2008). "Global metabolic profiling of Escherichia coli cultures: an evaluation of methods for quenching and extraction of intracellular metabolites." Anal Chem 80:2939-2948. Pubmed: 18331064
Synthesis Reference:Not Available
Material Safety Data Sheet (MSDS)Not Available
External Links:
ResourceLink
CHEBI ID17672
HMDB IDHMDB01096
Pubchem Compound ID278
Kegg IDC00169
ChemSpider ID272
WikipediaCarbamoyl phosphate
BioCyc IDCARBAMOYL-P
EcoCyc IDCARBAMOYL-P
Ligand ExpoCP

Enzymes

General function:
Involved in ATP binding
Specific function:
2 ATP + L-glutamine + HCO(3)(-) + H(2)O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate
Gene Name:
carB
Uniprot ID:
P00968
Molecular weight:
117841
Reactions
2 ATP + L-glutamine + HCO(3)(-) + H(2)O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.
General function:
Involved in carboxyl- or carbamoyltransferase activity
Specific function:
Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline
Gene Name:
argI
Uniprot ID:
P04391
Molecular weight:
36907
Reactions
Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.
General function:
Involved in carboxyl- or carbamoyltransferase activity
Specific function:
Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline
Gene Name:
argF
Uniprot ID:
P06960
Molecular weight:
36827
Reactions
Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.
General function:
Involved in glutamine catabolic process
Specific function:
2 ATP + L-glutamine + HCO(3)(-) + H(2)O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate
Gene Name:
carA
Uniprot ID:
P0A6F1
Molecular weight:
41431
Reactions
2 ATP + L-glutamine + HCO(3)(-) + H(2)O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.
General function:
Involved in carboxyl- or carbamoyltransferase activity
Specific function:
Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate
Gene Name:
pyrB
Uniprot ID:
P0A786
Molecular weight:
34427
Reactions
Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.
General function:
Involved in 'de novo' pyrimidine base biosynthetic process
Specific function:
Involved in allosteric regulation of aspartate carbamoyltransferase
Gene Name:
pyrI
Uniprot ID:
P0A7F3
Molecular weight:
17121
General function:
Involved in cellular amino acid biosynthetic process
Specific function:
ATP + NH(3) + CO(2) = ADP + carbamoyl phosphate
Gene Name:
arcC
Uniprot ID:
P37306
Molecular weight:
31644
Reactions
ATP + NH(3) + CO(2) = ADP + carbamoyl phosphate.
General function:
Involved in cellular amino acid biosynthetic process
Specific function:
Specific function unknown
Gene Name:
yahI
Uniprot ID:
P77624
Molecular weight:
33931
General function:
Involved in cellular amino acid biosynthetic process
Specific function:
Specific function unknown
Gene Name:
yqeA
Uniprot ID:
Q46807
Molecular weight:
33071