Glutamate racemase (P22634)

Identification
Name:Glutamate racemase
Synonyms:Not Available
Gene Name:murI
Enzyme Class:
Biological Properties
General Function:Involved in racemase and epimerase activity, acting on amino acids and derivatives
Specific Function:Provides the (R)-glutamate required for cell wall biosynthesis
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb
1.0D-Glutamic acid ↔ 1.0L-Glutamate
ReactionCard
1.0Thumb ↔ 1.0Thumb
1.0L-Glutamate ↔ 1.0D-Glutamic acid
ReactionCard
SMPDB Reactions:
1.0L-Glutamic acid+1.0Thumb ↔ 1.0Thumb
1.0L-Glutamic acid + 1.0L-Glutamate ↔ 1.0D-Glutamic acid
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb
1.0D-Glutamic acid ↔ 1.0L-Glutamate
ReactionCard
1.0Thumb ↔ 1.0Thumb
1.0L-Glutamate ↔ 1.0D-Glutamic acid
ReactionCard
Complex Reactions:
1.0Thumb → 1.0Thumb
1.0L-Glutamate → 1.0D-Glutamic acid
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB03339D-Glutamic acidMetaboCard
ECMDB00148L-GlutamateMetaboCard
GO Classification:
Function
amino-acid racemase activity
catalytic activity
glutamate racemase activity
isomerase activity
racemase and epimerase activity
racemase and epimerase activity, acting on amino acids and derivatives
Process
aminoglycan metabolic process
carbohydrate metabolic process
glycosaminoglycan metabolic process
metabolic process
peptidoglycan biosynthetic process
peptidoglycan metabolic process
polysaccharide metabolic process
primary metabolic process
Gene Properties
Blattner:b3967
Gene OrientationClockwise
Centisome Percentage:89.74
Left Sequence End4163451
Right Sequence End4164308
Gene Sequence:
>858 bp
TTGCTGAAGTATCGCCTGATATCTGCTTTTGTGTTAATACCCGTCGTCATCGCGGCGTTG
TTTCTGTTGCCGCCGGTGGGGTTCGCCATTGTAACGCTGGTGGTCTGCATGCTGGCAGCG
TGGGAATGGGGACAGCTTAGCGGTTTTACCACTCGTTCGCAGCGAGTATGGTTGGCGGTG
TTATGCGGGTTATTGTTGGCGCTGATGCTTTTTCTGTTGCCGGAATATCACCGAAATATT
CATCAACCGCTGGTTGAAATCTCACTTTGGGCTTCGCTGGGTTGGTGGATTGTCGCGCTA
TTGCTGGTGCTGTTTTACCCAGGTTCCGCAGCAATCTGGCGTAACTCTAAAACATTGCGC
CTTATTTTTGGCGTGCTAACCATTGTTCCCTTCTTCTGGGGCATGCTGGCGTTACGGGCC
TGGCACTATGACGAGAATCATTACAGTGGCGCAATATGGCTGCTCTATGTCATGATCCTG
GTATGGGGCGCTGACTCCGGCGCATATATGTTTGGCAAATTGTTTGGTAAACATAAGCTG
GCACCGAAGGTTTCTCCGGGTAAAACCTGGCAAGGCTTTATCGGTGGACTCGCTACTGCA
GCGGTAATCTCATGGGGTTATGGCATGTGGGCGAATCTCGACGTCGCTCCCGTCACCTTA
CTCATTTGCTCTATTGTCGCAGCGTTAGCCTCAGTGCTCGGCGATCTGACCGAGAGTATG
TTTAAGCGTGAAGCAGGAATTAAGGACAGCGGTCATTTAATTCCAGGACACGGTGGTATT
TTAGATCGTATTGATAGCCTGACGGCTGCGGTACCGGTCTTTGCTTGCTTGTTGTTACTG
GTATTCAGGACGCTTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:285
Protein Molecular Weight:31002
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Glutamate racemase
MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLLPDLHYIYAFDNVAFPY
GEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPALREKFDFPVVGVVPAIKP
AARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEMLGSAEMVELAEAKLHGEDVSL
DALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVLPEGTRLVDSGAAIARRTAWLLE
HEAPDAKSADANIAFCMAMTPGAEQLLPVLQRYGFETLEKLAVLG
References
External Links:
ResourceLink
Uniprot ID:P22634
Uniprot Name:MURI_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674368
Ecogene ID:EG11204
Ecocyc:EG11204
ColiBase:b3967
Kegg Gene:b3967
EchoBASE ID:EB1189
CCDB:MURI_ECOLI
BacMap:90111671
General Reference:
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Boros, I., Csordas-Toth, E., Kiss, A., Kiss, I., Torok, I., Udvardy, A., Udvardy, K., Venetianer, P. (1983). "Identification of two new promoters probably involved in the transcription of a ribosomal RNA gene of Escherichia coli." Biochim Biophys Acta 739:173-180. Pubmed: 6297582
  • Brosius, J., Dull, T. J., Sleeter, D. D., Noller, H. F. (1981). "Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli." J Mol Biol 148:107-127. Pubmed: 7028991
  • Doublet, P., van Heijenoort, J., Bohin, J. P., Mengin-Lecreulx, D. (1993). "The murI gene of Escherichia coli is an essential gene that encodes a glutamate racemase activity." J Bacteriol 175:2970-2979. Pubmed: 8098327
  • Doublet, P., van Heijenoort, J., Mengin-Lecreulx, D. (1992). "Identification of the Escherichia coli murI gene, which is required for the biosynthesis of D-glutamic acid, a specific component of bacterial peptidoglycan." J Bacteriol 174:5772-5779. Pubmed: 1355768
  • Dougherty, T. J., Thanassi, J. A., Pucci, M. J. (1993). "The Escherichia coli mutant requiring D-glutamic acid is the result of mutations in two distinct genetic loci." J Bacteriol 175:111-116. Pubmed: 8093236
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553