Identification
Name:Osmolarity sensor protein envZ
Synonyms:Not Available
Gene Name:envZ
Enzyme Class:
Biological Properties
General Function:Signal transduction mechanisms
Specific Function:Member of the two-component regulatory system envZ/ompR involved in the regulation of osmoregulation (genes ompF and ompC). EnvZ functions as a membrane-associated protein kinase that phosphorylates ompR in response to environmental signals
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane
membrane part
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
kinase activity
molecular transducer activity
nucleoside binding
protein histidine kinase activity
protein kinase activity
purine nucleoside binding
signal transducer activity
transferase activity
transferase activity, transferring phosphorus-containing groups
two-component sensor activity
Process
biological regulation
cellular metabolic process
metabolic process
peptidyl-histidine phosphorylation
phosphate metabolic process
phosphorus metabolic process
phosphorylation
protein amino acid phosphorylation
regulation of biological process
regulation of cellular process
signal transduction
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>1353 bp
ATGAGGCGATTGCGCTTCTCGCCACGAAGTTCATTTGCCCGTACGTTATTGCTCATCGTC
ACCTTGCTGTTCGCCAGCCTGGTGACGACTTATCTGGTGGTGCTGAACTTCGCGATTTTG
CCGAGCCTCCAGCAGTTTAATAAAGTCCTCGCGTACGAAGTGCGTATGTTGATGACCGAC
AAACTGCAACTGGAGGACGGCACGCAGTTGGTTGTGCCTCCCGCTTTCCGTCGGGAGATC
TACCGTGAGCTGGGGATCTCTCTCTACTCCAACGAGGCTGCCGAAGAGGCAGGTCTGCGT
TGGGCGCAACACTATGAATTCTTAAGCCATCAGATGGCGCAGCAACTGGGCGGCCCGACG
GAAGTGCGCGTTGAGGTCAACAAAAGTTCGCCTGTCGTCTGGCTGAAAACCTGGCTGTCG
CCCAATATCTGGGTACGCGTGCCGCTGACCGAAATTCATCAGGGCGATTTCTCTCCGCTG
TTCCGCTATACGCTGGCGATTATGCTATTGGCGATAGGCGGGGCGTGGCTGTTTATTCGT
ATCCAGAACCGACCGTTGGTCGATCTCGAACACGCAGCCTTGCAGGTTGGTAAAGGGATT
ATTCCGCCGCCGCTGCGTGAGTATGGCGCTTCGGAGGTGCGTTCCGTTACCCGTGCCTTT
AACCATATGGCGGCTGGTGTTAAGCAACTGGCGGATGACCGCACGCTGCTGATGGCGGGG
GTAAGTCACGACTTGCGCACGCCGCTGACGCGTATTCGCCTGGCGACTGAGATGATGAGC
GAGCAGGATGGCTATCTGGCAGAATCGATCAATAAAGATATCGAAGAGTGCAACGCCATC
ATTGAGCAGTTTATCGACTACCTGCGCACCGGGCAGGAGATGCCGATGGAAATGGCGGAT
CTTAATGCAGTACTCGGTGAGGTGATTGCTGCCGAAAGTGGCTATGAGCGGGAAATTGAA
ACCGCGCTTTACCCCGGCAGCATTGAAGTGAAAATGCACCCGCTGTCGATCAAACGCGCG
GTGGCGAATATGGTGGTCAACGCCGCCCGTTATGGCAATGGCTGGATCAAAGTCAGCAGC
GGAACGGAGCCGAATCGCGCCTGGTTCCAGGTGGAAGATGACGGTCCGGGAATTGCGCCG
GAACAACGTAAGCACCTGTTCCAGCCGTTTGTCCGCGGCGACAGTGCGCGCACCATTAGC
GGCACGGGATTAGGGCTGGCAATTGTGCAGCGTATCGTGGATAACCATAACGGGATGCTG
GAGCTTGGCACCAGCGAGCGGGGCGGGCTTTCCATTCGCGCCTGGCTGCCAGTGCCGGTA
ACGCGGGCGCAGGGCACGACAAAAGAAGGGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:450
Protein Molecular Weight:50334
Protein Theoretical pI:7
PDB File:1BXD
Signaling Regions:
  • None
Transmembrane Regions:
  • 16-35
  • 159-179
Protein Sequence:
>Osmolarity sensor protein envZ
MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQQFNKVLAYEVRMLMTD
KLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWAQHYEFLSHQMAQQLGGPT
EVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPLFRYTLAIMLLAIGGAWLFIR
IQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTRAFNHMAAGVKQLADDRTLLMAG
VSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEECNAIIEQFIDYLRTGQEMPMEMAD
LNAVLGEVIAAESGYEREIETALYPGSIEVKMHPLSIKRAVANMVVNAARYGNGWIKVSS
GTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVRGDSARTISGTGLGLAIVQRIVDNHNGML
ELGTSERGGLSIRAWLPVPVTRAQGTTKEG
References
External Links:
ResourceLink
Uniprot ID:P0AEJ4
Uniprot Name:ENVZ_ECOLI
GenBank Gene ID:J01656
Genebank Protein ID:147006
PDB ID:1BXD
CCDB:ENVZ_ECOLI
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Cai, S. J., Khorchid, A., Ikura, M., Inouye, M. (2003). "Probing catalytically essential domain orientation in histidine kinase EnvZ by targeted disulfide crosslinking." J Mol Biol 328:409-418. Pubmed: 12691749
  • Comeau, D. E., Ikenaka, K., Tsung, K. L., Inouye, M. (1985). "Primary characterization of the protein products of the Escherichia coli ompB locus: structure and regulation of synthesis of the OmpR and EnvZ proteins." J Bacteriol 164:578-584. Pubmed: 2997120
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Forst, S., Comeau, D., Norioka, S., Inouye, M. (1987). "Localization and membrane topology of EnvZ, a protein involved in osmoregulation of OmpF and OmpC in Escherichia coli." J Biol Chem 262:16433-16438. Pubmed: 2824492
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kanamaru, K., Aiba, H., Mizuno, T. (1990). "Transmembrane signal transduction and osmoregulation in Escherichia coli: I. Analysis by site-directed mutagenesis of the amino acid residues involved in phosphotransfer between the two regulatory components, EnvZ and OmpR." J Biochem 108:483-487. Pubmed: 2277041
  • Khorchid, A., Inouye, M., Ikura, M. (2005). "Structural characterization of Escherichia coli sensor histidine kinase EnvZ: the periplasmic C-terminal core domain is critical for homodimerization." Biochem J 385:255-264. Pubmed: 15357641
  • Kishii, R., Falzon, L., Yoshida, T., Kobayashi, H., Inouye, M. (2007). "Structural and functional studies of the HAMP domain of EnvZ, an osmosensing transmembrane histidine kinase in Escherichia coli." J Biol Chem 282:26401-26408. Pubmed: 17635923
  • Mizuno, T., Wurtzel, E. T., Inouye, M. (1982). "Osmoregulation of gene expression. II. DNA sequence of the envZ gene of the ompB operon of Escherichia coli and characterization of its gene product." J Biol Chem 257:13692-13698. Pubmed: 6292200
  • Roberts, D. L., Bennett, D. W., Forst, S. A. (1994). "Identification of the site of phosphorylation on the osmosensor, EnvZ, of Escherichia coli." J Biol Chem 269:8728-8733. Pubmed: 8132603
  • Tanaka, T., Saha, S. K., Tomomori, C., Ishima, R., Liu, D., Tong, K. I., Park, H., Dutta, R., Qin, L., Swindells, M. B., Yamazaki, T., Ono, A. M., Kainosho, M., Inouye, M., Ikura, M. (1998). "NMR structure of the histidine kinase domain of the E. coli osmosensor EnvZ." Nature 396:88-92. Pubmed: 9817206
  • Tokishita, S., Kojima, A., Mizuno, T. (1992). "Transmembrane signal transduction and osmoregulation in Escherichia coli: functional importance of the transmembrane regions of membrane-located protein kinase, EnvZ." J Biochem 111:707-713. Pubmed: 1323560
  • Tomomori, C., Tanaka, T., Dutta, R., Park, H., Saha, S. K., Zhu, Y., Ishima, R., Liu, D., Tong, K. I., Kurokawa, H., Qian, H., Inouye, M., Ikura, M. (1999). "Solution structure of the homodimeric core domain of Escherichia coli histidine kinase EnvZ." Nat Struct Biol 6:729-734. Pubmed: 10426948