Sulfurtransferase tusE (P0AB18)

Identification
Name:Sulfurtransferase tusE
Synonyms:
  • tRNA 2-thiouridine synthesizing protein E
Gene Name:tusE
Enzyme Class:Not Available
Biological Properties
General Function:Inorganic ion transport and metabolism
Specific Function:Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Could accept sulfur from tusD
Cellular Location:Cytoplasm (Probable)
SMPDB Pathways:Not Available
KEGG Pathways:
Metabolites:
ECMDB IDNameView
GO Classification:Not Available
Gene Properties
Blattner:b0969
Gene OrientationCounterclockwise
Centisome Percentage:22.19
Left Sequence End1029562
Right Sequence End1029891
Gene Sequence:
>330 bp
ATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAA
ATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGT
CTGGTAAAAGTGACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGC
CTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGACGCA
GCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAG
CTGCCGCCTGGCTTTAAGATGCCGTTCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:109
Protein Molecular Weight:12410
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Sulfurtransferase tusE
MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHWEVVRFVRDFYLEFNTS
PAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGLPKPVKCI
References
External Links:
ResourceLink
Uniprot ID:P0AB18
Uniprot Name:TUSE_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674610
Ecogene ID:EG12876
Ecocyc:EG12876
ColiBase:b0969
Kegg Gene:b0969
EchoBASE ID:EB2714
CCDB:TUSE_ECOLI
BacMap:90111197
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Borodovsky, M., McIninch, J. D., Koonin, E. V., Rudd, K. E., Medigue, C., Danchin, A. (1995). "Detection of new genes in a bacterial genome using Markov models for three gene classes." Nucleic Acids Res 23:3554-3562. Pubmed: 7567469
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Ikeuchi, Y., Shigi, N., Kato, J., Nishimura, A., Suzuki, T. (2006). "Mechanistic insights into sulfur relay by multiple sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positions." Mol Cell 21:97-108. Pubmed: 16387657
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Tamura, F., Nishimura, S., Ohki, M. (1984). "The E. coli divE mutation, which differentially inhibits synthesis of certain proteins, is in tRNASer1." EMBO J 3:1103-1107. Pubmed: 6376117