Ribosomal RNA large subunit methyltransferase H (P0A8I8)

Identification
Name:Ribosomal RNA large subunit methyltransferase H
Synonyms:
  • 23S rRNA m3Psi1915 methyltransferase
  • rRNA (pseudouridine-N3-)-methyltransferase rlmH
Gene Name:rlmH
Enzyme Class:
Biological Properties
General Function:Involved in protein homodimerization activity
Specific Function:Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA. Specific for fully assembled 70S ribosomes
Cellular Location:Cytoplasm (Potential)
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
ECMDB00939S-AdenosylhomocysteineMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
catalytic activity
methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
rRNA metabolic process
rRNA methylation
rRNA modification
rRNA processing
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>468 bp
GTGAAGCTGCAACTTGTCGCCGTGGGAACGAAAATGCCGGACTGGGTACAAACCGGTTTT
ACCGAGTACCTGCGTCGTTTTCCGAAAGATATGCCCTTCGAGCTGATTGAAATTCCGGCC
GGAAAACGCGGCAAGAATGCGGACATCAAGCGCATACTCGACAAAGAGGGTGAGCAGATG
TTGGCGGCCGCAGGCAAAAACCGCATTGTCACCCTCGATATTCCAGGCAAGCCCTGGGAT
ACGCCGCAGTTAGCCGCTGAGCTGGAACGCTGGAAGCTGGATGGTCGCGACGTCAGTCTA
CTGATTGGCGGGCCTGAAGGGTTGTCGCCTGCCTGTAAAGCGGCGGCTGAGCAGAGCTGG
TCGCTGTCGGCGCTTACCCTCCCCCATCCGCTGGTTCGCGTGCTGGTCGCAGAGAGTCTG
TACCGGGCGTGGAGCATCACCACCAACCATCCTTATCACCGTGAGTGA
Protein Properties
Pfam Domain Function:
Protein Residues:155
Protein Molecular Weight:17341
Protein Theoretical pI:9
PDB File:1NS5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Ribosomal RNA large subunit methyltransferase H
MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRGKNADIKRILDKEGEQM
LAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLIGGPEGLSPACKAAAEQSW
SLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE
References
External Links:
ResourceLink
Uniprot ID:P0A8I8
Uniprot Name:RLMH_ECOLI
GenBank Gene ID:U00096
Genebank Protein ID:1786855
PDB ID:1NS5
CCDB:RLMH_ECOLI
General Reference:
  • Asoh, S., Matsuzawa, H., Ishino, F., Strominger, J. L., Matsuhashi, M., Ohta, T. (1986). "Nucleotide sequence of the pbpA gene and characteristics of the deduced amino acid sequence of penicillin-binding protein 2 of Escherichia coli K12." Eur J Biochem 160:231-238. Pubmed: 3533535
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Ero, R., Peil, L., Liiv, A., Remme, J. (2008). "Identification of pseudouridine methyltransferase in Escherichia coli." RNA 14:2223-2233. Pubmed: 18755836
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Purta, E., Kaminska, K. H., Kasprzak, J. M., Bujnicki, J. M., Douthwaite, S. (2008). "YbeA is the m3Psi methyltransferase RlmH that targets nucleotide 1915 in 23S rRNA." RNA 14:2234-2244. Pubmed: 18755835