Crossover junction endodeoxyribonuclease ruvC (P0A814)

Identification
Name:Crossover junction endodeoxyribonuclease ruvC
Synonyms:
  • Holliday junction nuclease ruvC
  • Holliday junction resolvase ruvC
Gene Name:ruvC
Enzyme Class:
Biological Properties
General Function:Involved in nucleic acid binding
Specific Function:Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:
Metabolites:
ECMDB IDNameView
GO Classification:
Function
binding
catalytic activity
endodeoxyribonuclease activity
endonuclease activity
hydrolase activity
hydrolase activity, acting on ester bonds
nuclease activity
nucleic acid binding
Process
cellular macromolecule metabolic process
DNA metabolic process
DNA recombination
DNA repair
macromolecule metabolic process
metabolic process
Gene Properties
Blattner:b1863
Gene OrientationCounterclockwise
Centisome Percentage:41.92
Left Sequence End1944879
Right Sequence End1945400
Gene Sequence:
>522 bp
ATGCAACTGAACAAAGTGCTGAAAGGGCTGATGATTGCTCTGCCTGTTATGGCAATTGCG
GCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGC
ACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTG
CAAATGCAACAGCTGCAGCAGAACAACATCGTTTACTTCGATCTGGACAAGTACGATATC
CGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCT
TACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCC
CTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCA
GACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAA
GCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:173
Protein Molecular Weight:18747
Protein Theoretical pI:10
PDB File:1HJR
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Crossover junction endodeoxyribonuclease ruvC
MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSRLKLIYAGVTEIITQFQ
PDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEYAARQVKQTVVGIGSAEKS
QVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQMSESRLNLARGRLR
References
External Links:
ResourceLink
Uniprot ID:P0A814
Uniprot Name:RUVC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651333
PDB ID:1HJR
Ecogene ID:EG10925
Ecocyc:EG10925
ColiBase:b1863
Kegg Gene:b1863
EchoBASE ID:EB0918
CCDB:RUVC_ECOLI
BacMap:16129816
General Reference:
  • Ariyoshi, M., Vassylyev, D. G., Iwasaki, H., Nakamura, H., Shinagawa, H., Morikawa, K. (1994). "Atomic structure of the RuvC resolvase: a holliday junction-specific endonuclease from E. coli." Cell 78:1063-1072. Pubmed: 7923356
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Dunderdale, H. J., Benson, F. E., Parsons, C. A., Sharples, G. J., Lloyd, R. G., West, S. C. (1991). "Formation and resolution of recombination intermediates by E. coli RecA and RuvC proteins." Nature 354:506-510. Pubmed: 1758493
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Iwasaki, H., Takahagi, M., Shiba, T., Nakata, A., Shinagawa, H. (1991). "Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure." EMBO J 10:4381-4389. Pubmed: 1661673
  • Sharples, G. J., Lloyd, R. G. (1991). "Resolution of Holliday junctions in Escherichia coli: identification of the ruvC gene product as a 19-kilodalton protein." J Bacteriol 173:7711-7715. Pubmed: 1657895
  • Takahagi, M., Iwasaki, H., Nakata, A., Shinagawa, H. (1991). "Molecular analysis of the Escherichia coli ruvC gene, which encodes a Holliday junction-specific endonuclease." J Bacteriol 173:5747-5753. Pubmed: 1885548
  • West, S. C. (1997). "Processing of recombination intermediates by the RuvABC proteins." Annu Rev Genet 31:213-244. Pubmed: 9442895