Methylglyoxal synthase (P0A731)

Identification
Name:Methylglyoxal synthase
Synonyms:
  • MGS
Gene Name:mgsA
Enzyme Class:
Biological Properties
General Function:Involved in methylglyoxal synthase activity
Specific Function:Glycerone phosphate = methylglyoxal + phosphate
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Dihydroxyacetone phosphate ↔ 1.0Pyruvaldehyde + 1.0Phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Dihydroxyacetone phosphate ↔ 1.0Pyruvaldehyde + 1.0Phosphate
ReactionCard
Complex Reactions:
1.0Thumb → 1.0Thumb+1.0Thumb
1.0Dihydroxyacetone phosphate → 1.0Pyruvaldehyde + 1.0Inorganic phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01473Dihydroxyacetone phosphateMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB01167PyruvaldehydeMetaboCard
GO Classification:
Function
carbon-oxygen lyase activity
carbon-oxygen lyase activity, acting on phosphates
catalytic activity
lyase activity
methylglyoxal synthase activity
Process
cellular aldehyde metabolic process
cellular metabolic process
metabolic process
methylglyoxal biosynthetic process
methylglyoxal metabolic process
Gene Properties
Blattner:b0963
Gene OrientationCounterclockwise
Centisome Percentage:22.11
Left Sequence End1025780
Right Sequence End1026238
Gene Sequence:
>459 bp
ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACC
CGTTATCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATT
TATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTA
TCGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCAT
GCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAATCGCGCCAGTACTGCGGCAA
CATGCCGGGCTGACAAAAGAAGTGATGCTGGTTGGACAGTTCAACAAGTTTGAGCTGTGG
GATGAAACAACCTGGCATCAACAGGTCAAGGAAGATATCGACGCAGAGCAGTTGGCTACC
GGAGACTTATCGGAGCGACTGCAGGACTTGTCTCTATAA
Protein Properties
Pfam Domain Function:
Protein Residues:152
Protein Molecular Weight:16918
Protein Theoretical pI:7
PDB File:1IK4
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Methylglyoxal synthase
MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYATGTTGNLISRATGMNV
NAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDVKALLRLATVWNIPVATNV
ATADFIIQSPHFNDAVDILIPDYQRYLADRLK
References
External Links:
ResourceLink
Uniprot ID:P0A731
Uniprot Name:MGSA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321962
PDB ID:1IK4
Ecogene ID:EG12307
Ecocyc:EG12307
ColiBase:b0963
Kegg Gene:b0963
EchoBASE ID:EB2213
CCDB:MGSA_ECOLI
BacMap:90111195
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Borodovsky, M., Rudd, K. E., Koonin, E. V. (1994). "Intrinsic and extrinsic approaches for detecting genes in a bacterial genome." Nucleic Acids Res 22:4756-4767. Pubmed: 7984428
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Saadat, D., Harrison, D. H. (1998). "Identification of catalytic bases in the active site of Escherichia coli methylglyoxal synthase: cloning, expression, and functional characterization of conserved aspartic acid residues." Biochemistry 37:10074-10086. Pubmed: 9665712
  • Saadat, D., Harrison, D. H. (1999). "The crystal structure of methylglyoxal synthase from Escherichia coli." Structure 7:309-317. Pubmed: 10368300
  • Saadat, D., Harrison, D. H. (2000). "Mirroring perfection: the structure of methylglyoxal synthase complexed with the competitive inhibitor 2-phosphoglycolate." Biochemistry 39:2950-2960. Pubmed: 10715115
  • Totemeyer, S., Booth, N. A., Nichols, W. W., Dunbar, B., Booth, I. R. (1998). "From famine to feast: the role of methylglyoxal production in Escherichia coli." Mol Microbiol 27:553-562. Pubmed: 9489667
  • Wood, E. R., Matson, S. W. (1989). "The molecular cloning of the gene encoding the Escherichia coli 75-kDa helicase and the determination of its nucleotide sequence and gentic map position." J Biol Chem 264:8297-8303. Pubmed: 2542273