Displaying growth conditions 1001 - 1025 of 1187 in total
ECMDB IDName ConcentrationStrain
Media
Growth Status
Growth System
TemperatureCitation
ECMDB00696L-Methionine129± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glycerol
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00696L-Methionine66± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L acetate
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00696L-Methionine30± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
ECMDB00696L-Methionine58± 7 uMBL21 DE3
Luria-Bertani (LB) media
Stationary phase cultures (overnight culture)
Shake flask
37 oCExperimentally Determined
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ECMDB00696L-Methionine43± 5 uMBL21 DE3
Luria-Bertani (LB) media
Stationary phase cultures (overnight culture)
Shake flask
37 oCExperimentally Determined
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ECMDB00159L-Phenylalanine18± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00159L-Phenylalanine42± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glycerol
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00159L-Phenylalanine27± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L acetate
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00159L-Phenylalanine66± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
ECMDB00159L-Phenylalanine52± 4 uMBL21 DE3
Luria-Bertani (LB) media
Stationary phase cultures (overnight culture)
Shake flask
37 oCExperimentally Determined
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ECMDB00162L-Proline385± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00162L-Proline451± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glycerol
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00162L-Proline361± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L acetate
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00162L-Proline143± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
ECMDB00162L-Proline550± 45 uMBL21 DE3
Luria-Bertani (LB) media
Stationary phase cultures (overnight culture)
Shake flask
37 oCExperimentally Determined
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ECMDB00162L-Proline532± 11 uMBL21 DE3
Luria-Bertani (LB) media
Stationary phase cultures (overnight culture)
Shake flask
37 oCExperimentally Determined
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ECMDB20167L-Ribulose 5-phosphate2± 0 uMW3110
4.0 g/L Na2SO4; 5.36 g/L (NH4)2SO4; 1.0 g/L NH4Cl; 7.3 g/L K2HPO4; 1.8 g/L NaH2PO4 H2O; 12.0 g/L (NH4)2-H-citrate; 4.0 mL/L MgSO4 (1 M); 6.0 mL/L trace element solution; 0.02 g/L thiamine, 20 g/L glucose
Mid Log Phase
Bioreactor, pH controlled, aerated, dilution rate=0.125 L/h
37 oCPark, C., Park, C., Lee, Y., Lee, S.Y., Oh, H.B., Lee, J. (2011) Determination of the Intracellular Concentration of Metabolites in Escherichia coli Collected during the Exponential and Stationary Growth Phases using Liquid Chromatography-Mass Spectrometry. Bull Korean Chem. Soc. 32: 524-530.
ECMDB20167L-Ribulose 5-phosphate2± 0 uMW3110
4.0 g/L Na2SO4; 5.36 g/L (NH4)2SO4; 1.0 g/L NH4Cl; 7.3 g/L K2HPO4; 1.8 g/L NaH2PO4 H2O; 12.0 g/L (NH4)2-H-citrate; 4.0 mL/L MgSO4 (1 M); 6.0 mL/L trace element solution; 0.02 g/L thiamine, 20 g/L glucose
Stationary Phase
Bioreactor, pH controlled, aerated
37 oCPark, C., Park, C., Lee, Y., Lee, S.Y., Oh, H.B., Lee, J. (2011) Determination of the Intracellular Concentration of Metabolites in Escherichia coli Collected during the Exponential and Stationary Growth Phases using Liquid Chromatography-Mass Spectrometry. Bull Korean Chem. Soc. 32: 524-530.
ECMDB00187L-Serine68± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00187L-Serine150± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glycerol
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00187L-Serine96± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L acetate
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00187L-Serine343± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
ECMDB00187L-Serine48± 4 uMBL21 DE3
Luria-Bertani (LB) media
Stationary phase cultures (overnight culture)
Shake flask
37 oCExperimentally Determined
Download Details
ECMDB00167L-Threonine179± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00167L-Threonine147± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
Displaying growth conditions 1001 - 1025 of 1187 in total