2.0 2015-09-08 19:52:11 -0600 2015-12-09 12:12:34 -0700 ECMDB24637 M2MDB006754 CDP-DG(10:0(3-OH)/19:0cycv8c) CDP-DG(10:0(3-OH)/19:0cycv8c) belongs to the family of CDP-diacylglycerols. It is a glycerophospholipid containing a diacylglycerol, with a cytidine diphosphate attached to the oxygen O1 or O2 of the glycerol part. As is the case with diacylglycerols, phosphatidylserines can have many different combinations of fatty acids of varying lengths and saturation attached to the C-1 and C-2 atoms. CDP-DG(10:0(3-OH)/19:0cycv8c), in particular, consists of one 3-hydroxydecanoyl chain to C-1 atom, and one heptadec-11-12-cyclo-anoyl to the C-2 atom. In E. coli glycerophospholipid metabolism, The biosynthesis of CDP-diacylglycerol (CDP-DG) involves condensation of phosphatidic acid (PA) and cytidine triphosphate, with elimination of pyrophosphate, catalysed by the enzyme CDP-diacylglycerol synthase. The resulting CDP-diacylglycerol can be utilized immediately for the synthesis of phosphatidylglycerol (PG), and thence cardiolipin (CL), and of phosphatidylinositol (PI). CDP-DG(10:0(3-OH)/19:0cycv8c) is also a substrate of CDP-diacylglycerol pyrophosphatase. It is involved in CDP-diacylglycerol degradation pathway. C41H73N3O16P2 925.988 925.446607279 {[(2R,3R,5R)-5-(4-amino-2-oxo-1,2-dihydropyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methoxy}({[(2R)-2-{[10-(2-hexylcyclopropyl)decanoyl]oxy}-3-[(3-hydroxydecanoyl)oxy]propoxy](hydroxy)phosphoryl}oxy)phosphinic acid [(2R,3R,5R)-5-(4-amino-2-oxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methoxy([(2R)-2-{[10-(2-hexylcyclopropyl)decanoyl]oxy}-3-[(3-hydroxydecanoyl)oxy]propoxy(hydroxy)phosphoryl]oxy)phosphinic acid [H][C@@](COC(=O)CC(O)CCCCCCC)(COP(O)(=O)OP(O)(=O)OC[C@H]1O[C@H](C(O)[C@H]1O)N1C=CC(N)=NC1=O)OC(=O)CCCCCCCCCC1CC1CCCCCC InChI=1S/C41H73N3O16P2/c1-3-5-7-12-17-21-32(45)26-37(47)55-27-33(58-36(46)22-18-14-11-9-10-13-16-20-31-25-30(31)19-15-8-6-4-2)28-56-61(51,52)60-62(53,54)57-29-34-38(48)39(49)40(59-34)44-24-23-35(42)43-41(44)50/h23-24,30-34,38-40,45,48-49H,3-22,25-29H2,1-2H3,(H,51,52)(H,53,54)(H2,42,43,50)/t30?,31?,32?,33-,34-,38+,39?,40-/m1/s1 ARSWGGNVRYYNPU-VGXUDMJPSA-N logp 4.22 ALOGPS logs -4.45 ALOGPS solubility 3.27e-02 g/l ALOGPS logp 6.21 ChemAxon pka_strongest_acidic -0.032 ChemAxon pka_strongest_basic 3.27 ChemAxon iupac {[(2R,3R,5R)-5-(4-amino-2-oxo-1,2-dihydropyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methoxy}({[(2R)-2-{[10-(2-hexylcyclopropyl)decanoyl]oxy}-3-[(3-hydroxydecanoyl)oxy]propoxy](hydroxy)phosphoryl}oxy)phosphinic acid ChemAxon average_mass 925.988 ChemAxon mono_mass 925.446607279 ChemAxon smiles [H][C@@](COC(=O)CC(O)CCCCCCC)(COP(O)(=O)OP(O)(=O)OC[C@H]1O[C@H](C(O)[C@H]1O)N1C=CC(N)=NC1=O)OC(=O)CCCCCCCCCC1CC1CCCCCC ChemAxon formula C41H73N3O16P2 ChemAxon inchi InChI=1S/C41H73N3O16P2/c1-3-5-7-12-17-21-32(45)26-37(47)55-27-33(58-36(46)22-18-14-11-9-10-13-16-20-31-25-30(31)19-15-8-6-4-2)28-56-61(51,52)60-62(53,54)57-29-34-38(48)39(49)40(59-34)44-24-23-35(42)43-41(44)50/h23-24,30-34,38-40,45,48-49H,3-22,25-29H2,1-2H3,(H,51,52)(H,53,54)(H2,42,43,50)/t30?,31?,32?,33-,34-,38+,39?,40-/m1/s1 ChemAxon inchikey ARSWGGNVRYYNPU-VGXUDMJPSA-N ChemAxon polar_surface_area 283.5 ChemAxon refractivity 226.02 ChemAxon polarizability 99.09 ChemAxon rotatable_bond_count 37 ChemAxon acceptor_count 13 ChemAxon donor_count 6 ChemAxon physiological_charge -2 ChemAxon formal_charge 0 ChemAxon phospholipid biosynthesis (CL(10:0(3-OH)/19:0cycv8c/10:0(3-OH)/19:0cycv8c)) Phospholipids are membrane components in E. coli. The major phospholipids of E. coli are phosphatidylethanolamine, phosphatidylglycerol and cardiolipin. All phospholipids contain sn-glycerol-3-phosphate esterified with fatty acids at the sn-1 and sn-2 positions. The reaction starts from a glycerone phosphate (dihydroxyacetone phosphate) produced in glycolysis. The glycerone phosphate is transformed to a sn-glycerol 3-phosphate (glycerol 3 phosphate) by NADPH driven glycerol-3-phosphate dehydrogenase. Sn-glycerol 3-phosphate is transformed to a 1-acyl-sn-glycerol 3-phosphate(1-oleyl-2-lyso-phosphatidate , 1-palmitoylglycerol 3-phosphate , 1-stearoyl-sn-glycerol 3-phosphate). This can be achieve by a sn-glycerol-3-phosphate 1-0-acyltransferase that interacts either with a long-chain acyl-CoA or with an acyl-[acp]. The 1-acyl-sn-glycerol 3-phosphate is transformed into a 1,2-diacyl-sn-glycerol 3-phosphate through a 1-acylglycerol-3-phosphate O-acyltransferase. This compound is then converted into a CPD-diacylglycerol through a CTP (phosphatidate cytididyltransferase. CPD-diacylglycerol can be transformed either to a L-1-phosphatidylserine or a L-1-phosphatidylglycerol-phosphate through a phosphatidylserine synthase or a phosphatidylglycerophosphate synthase respectively. The L-1-phosphatidylserine transforms into L-1-phosphatidylethanolamine through a phosphatidylserine decarboxylase, o the other hand L-1-phosphatidylglycerol-phosphate gets transformed into a L-1-phosphatidyl-glycerol through a phosphatidylglycerophosphatase. These 2 products combines produce a cardiolipin and a ethanolamine. The L-1 phosphatidyl-glycerol can also interact with cardiolipin synthase resulting in a glycerol and a cardiolipin. PW001908 Metabolic Specdb::EiMs 4362 Specdb::NmrOneD 267188 Specdb::NmrOneD 267189 Specdb::NmrOneD 267190 Specdb::NmrOneD 267191 Specdb::NmrOneD 267192 Specdb::NmrOneD 267193 Specdb::NmrOneD 267194 Specdb::NmrOneD 267195 Specdb::NmrOneD 267196 Specdb::NmrOneD 267197 Specdb::NmrOneD 267198 Specdb::NmrOneD 267199 Specdb::NmrOneD 267200 Specdb::NmrOneD 267201 Specdb::NmrOneD 267202 Specdb::NmrOneD 267203 Specdb::NmrOneD 267204 Specdb::NmrOneD 267205 Specdb::NmrOneD 267206 Specdb::NmrOneD 267207 Specdb::MsMs 1309861 Specdb::MsMs 1309862 Specdb::MsMs 1309863 Specdb::MsMs 1424362 Specdb::MsMs 1424363 Specdb::MsMs 1424364 Keseler, I. M., Collado-Vides, J., Santos-Zavaleta, A., Peralta-Gil, M., Gama-Castro, S., Muniz-Rascado, L., Bonavides-Martinez, C., Paley, S., Krummenacker, M., Altman, T., Kaipa, P., Spaulding, A., Pacheco, J., Latendresse, M., Fulcher, C., Sarker, M., Shearer, A. G., Mackie, A., Paulsen, I., Gunsalus, R. P., Karp, P. D. (2011). "EcoCyc: a comprehensive database of Escherichia coli biology." Nucleic Acids Res 39:D583-D590. 21097882 Kanehisa, M., Goto, S., Sato, Y., Furumichi, M., Tanabe, M. (2012). "KEGG for integration and interpretation of large-scale molecular data sets." Nucleic Acids Res 40:D109-D114. 22080510 Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. 22102590 Yurtsever D. (2007). Fatty acid methyl ester profiling of Enterococcus and Esherichia coli for microbial source tracking. M.sc. Thesis. Villanova University: U.S.A CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase P0ABF8 PGSA_ECOLI pgsA http://ecmdb.ca/proteins/P0ABF8.xml Phosphatidate cytidylyltransferase P0ABG1 CDSA_ECOLI cdsA http://ecmdb.ca/proteins/P0ABG1.xml 2 DG(10:0(3-OH)/19:0cycv8c/0:0) + Cytidine triphosphate + Hydrogen ion >2 CDP-DG(10:0(3-OH)/19:0cycv8c) + Pyrophosphate PW_R005314 2 CDP-DG(10:0(3-OH)/19:0cycv8c) + Glycerol 3-phosphate >2 PGP(10:0(3-OH)/19:0cycv8c) + Cytidine monophosphate + Hydrogen ion + Cytidine monophosphate PW_R005316