Selenate (ECMDB23788) (M2MDB004318)

General function:

Involved in oxidoreductase activity

Specific function:

The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The alpha chain is the actual site of nitrate reduction

Gene Name:

narG

Uniprot ID:

P09152

Molecular weight:

140489

Reactions

General function:

Involved in nitrate reductase activity

Specific function:

This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit

Gene Name:

narV

Uniprot ID:

P0AF32

Molecular weight:

26018

Reactions

General function:

Involved in iron-sulfur cluster binding

Specific function:

The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit

Gene Name:

narH

Uniprot ID:

P11349

Molecular weight:

58066

Reactions

General function:

Involved in nitrate reductase activity

Specific function:

The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit

Gene Name:

narI

Uniprot ID:

P11350

Molecular weight:

25497

Reactions

General function:

Involved in unfolded protein binding

Specific function:

Chaperone required for proper molybdenum cofactor insertion and final assembly of the membrane-bound respiratory nitrate reductase 2

Gene Name:

narW

Uniprot ID:

P19317

Molecular weight:

26160

General function:

Involved in iron-sulfur cluster binding

Specific function:

This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit

Gene Name:

narY

Uniprot ID:

P19318

Molecular weight:

58557

Reactions

General function:

Involved in oxidoreductase activity

Specific function:

The alpha chain is the actual site of nitrate reduction

Gene Name:

narZ

Uniprot ID:

P19319

Molecular weight:

140226

Reactions

General function:

Involved in catalytic activity

Specific function:

ATP + sulfate = diphosphate + adenylyl sulfate

Gene Name:

cysD

Uniprot ID:

P21156

Molecular weight:

35188

Reactions

General function:

Involved in GTPase activity

Specific function:

May be the GTPase, regulating ATP sulfurylase activity

Gene Name:

cysN

Uniprot ID:

P23845

Molecular weight:

52558

Reactions

General function:

Involved in oxidoreductase activity

Specific function:

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism

Gene Name:

napA

Uniprot ID:

P33937

Molecular weight:

93041

Reactions

General function:

Energy production and conversion

Specific function:

Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme napC protein, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism

Gene Name:

napB

Uniprot ID:

P0ABL3

Molecular weight:

16297

General function:

Involved in unfolded protein binding

Specific function:

Chaperone required for proper molybdenum cofactor insertion and final assembly of the membrane-bound respiratory nitrate reductase 1. Required for the insertion of the molybdenum into the apo-NarG subunit, maybe by keeping NarG in an appropriate competent-open conformation for the molybdenum cofactor insertion to occur. NarJ maintains the apoNarGH complex in a soluble state. Upon insertion of the molybdenum cofactor, NarJ seems to dissociate from the activated soluble NarGH complex, before its association with the NarI subunit on the membrane

Gene Name:

narJ

Uniprot ID:

P0AF26

Molecular weight:

26449