2-Demethylmenaquinol 8 (ECMDB21154) (M2MDB001563)

General function:

Involved in electron carrier activity

Specific function:

Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; the fumarate reductase is used in anaerobic growth, and the succinate dehydrogenase is used in aerobic growth

Gene Name:

frdA

Uniprot ID:

P00363

Molecular weight:

65971

Reactions

General function:

Involved in oxidation-reduction process

Specific function:

Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Does not couple the redox reaction to proton translocation

Gene Name:

ndh

Uniprot ID:

P00393

Molecular weight:

47358

Reactions

General function:

Energy production and conversion

Specific function:

Seems to be involved in the anchoring of the catalytic components of the fumarate reductase complex to the cytoplasmic membrane

Gene Name:

frdC

Uniprot ID:

P0A8Q0

Molecular weight:

15015

General function:

Involved in fumarate metabolic process

Specific function:

Seems to be involved in the anchoring of the catalytic components of the fumarate reductase complex to the cytoplasmic membrane

Gene Name:

frdD

Uniprot ID:

P0A8Q3

Molecular weight:

13107

General function:

Involved in iron-sulfur cluster binding

Specific function:

Electron transfer protein; may also function as the membrane anchor for the glpAB dimer

Gene Name:

glpC

Uniprot ID:

P0A996

Molecular weight:

44108

General function:

Involved in oxidoreductase activity

Specific function:

Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses fumarate or nitrate as electron acceptor

Gene Name:

glpA

Uniprot ID:

P0A9C0

Molecular weight:

58958

Reactions

General function:

Involved in electron carrier activity

Specific function:

Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; the fumarate reductase is used in anaerobic growth, and the succinate dehydrogenase is used in aerobic growth

Gene Name:

frdB

Uniprot ID:

P0AC47

Molecular weight:

27123

Reactions

General function:

Involved in nickel cation binding

Specific function:

This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD1 is believed to have a role in hydrogen cycling during fermentative growth

Gene Name:

hyaB

Uniprot ID:

P0ACD8

Molecular weight:

66253

Reactions

General function:

Involved in nickel cation binding

Specific function:

This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake

Gene Name:

hybC

Uniprot ID:

P0ACE0

Molecular weight:

62491

Reactions

General function:

Involved in catalytic activity

Specific function:

Specific function unknown

Gene Name:

glcD

Uniprot ID:

P0AEP9

Molecular weight:

53811

General function:

Involved in oxidoreductase activity, acting on NADH or NADPH

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Gene Name:

nuoA

Uniprot ID:

P0AFC3

Molecular weight:

16457

Reactions

General function:

Involved in NADH dehydrogenase (ubiquinone) activity

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Gene Name:

nuoB

Uniprot ID:

P0AFC7

Molecular weight:

25056

Reactions

General function:

Involved in oxidoreductase activity

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Gene Name:

nuoE

Uniprot ID:

P0AFD1

Molecular weight:

18590

Reactions

General function:

Involved in oxidation-reduction process

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone

Gene Name:

nuoH

Uniprot ID:

P0AFD4

Molecular weight:

36219

Reactions

General function:

Involved in electron carrier activity

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Gene Name:

nuoI

Uniprot ID:

P0AFD6

Molecular weight:

20538

Reactions

General function:

Involved in NADH dehydrogenase (ubiquinone) activity

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Gene Name:

nuoJ

Uniprot ID:

P0AFE0

Molecular weight:

19874

Reactions

General function:

Involved in oxidoreductase activity, acting on NADH or NADPH

Specific function:

There are 2 NADH dehydrogenases in E.coli, however only this complex is able to use dNADH (reduced nicotinamide hypoxanthine dinucleotide, deamino-NADH) and dNADH-DB (dimethoxy- 5-methyl-6-decyl-1,4-benzoquinone) as substrates

Gene Name:

nuoK

Uniprot ID:

P0AFE4

Molecular weight:

10845

Reactions

General function:

Involved in NADH dehydrogenase (ubiquinone) activity

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Gene Name:

nuoM

Uniprot ID:

P0AFE8

Molecular weight:

56524

Reactions

General function:

Involved in NADH dehydrogenase (ubiquinone) activity

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Gene Name:

nuoN

Uniprot ID:

P0AFF0

Molecular weight:

52044

Reactions

General function:

Involved in electron carrier activity

Specific function:

Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses fumarate or nitrate as electron acceptor

Gene Name:

glpB

Uniprot ID:

P13033

Molecular weight:

45357

Reactions

General function:

Involved in dimethyl sulfoxide reductase activity

Specific function:

Terminal reductase during anaerobic growth on various sulfoxide and N-oxide compounds. Allows E.coli to grow anaerobically on Me(2)SO as respiratory oxidant

Gene Name:

dmsA

Uniprot ID:

P18775

Molecular weight:

90398

Reactions

General function:

Involved in electron carrier activity

Specific function:

Electron transfer subunit of the terminal reductase during anaerobic growth on various sulfoxide and N-oxide compounds

Gene Name:

dmsB

Uniprot ID:

P18776

Molecular weight:

22869

General function:

Involved in NADH dehydrogenase (ubiquinone) activity

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Gene Name:

nuoF

Uniprot ID:

P31979

Molecular weight:

49292

Reactions

General function:

Involved in transferase activity

Specific function:

Conversion of 1,4-dihydroxy-2-naphthoate (DHNA) to dimethylmenaquinone (DMK). Attaches octaprenylpyrophosphate, a membrane-bound 40-carbon side chain to DHNA. The conversion of DHNA to DMK proceeds in three stages:the removal of the carboxyl group of DHNA as CO(2), the attachment of the isoprenoid side chain, and a quinol-to-quinone oxidation, which is thought to be spontaneous

Gene Name:

menA

Uniprot ID:

P32166

Molecular weight:

33594

Reactions

General function:

Involved in oxidoreductase activity, acting on NADH or NADPH

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Gene Name:

nuoC

Uniprot ID:

P33599

Molecular weight:

68236

Reactions

General function:

Involved in electron carrier activity

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Gene Name:

nuoG

Uniprot ID:

P33602

Molecular weight:

100298

Reactions

General function:

Involved in NADH dehydrogenase (ubiquinone) activity

Specific function:

NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient

Gene Name:

nuoL

Uniprot ID:

P33607

Molecular weight:

66438

Reactions

General function:

Involved in iron-sulfur cluster binding

Specific function:

Specific function unknown

Gene Name:

glcF

Uniprot ID:

P52074

Molecular weight:

45110

General function:

Involved in NADH dehydrogenase (ubiquinone) activity

Specific function:

This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD1 is believed to have a role in hydrogen cycling during fermentative growth

Gene Name:

hyaA

Uniprot ID:

P69739

Molecular weight:

40681

Reactions

General function:

Involved in NADH dehydrogenase (ubiquinone) activity

Specific function:

This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake

Gene Name:

hybO

Uniprot ID:

P69741

Molecular weight:

39652

Reactions

General function:

Involved in oxidoreductase activity

Specific function:

Reduces trimethylamine-N-oxide (TMAO) into trimethylamine; an anaerobic reaction coupled to energy-yielding reactions. Can also reduce other N- and S-oxide compounds such as 4-methylmorpholine-N-oxide and biotin sulfoxide (BSO), but with a lower catalytic efficiency

Gene Name:

torZ

Uniprot ID:

P46923

Molecular weight:

88964

Reactions

General function:

Involved in coenzyme binding

Specific function:

Specific function unknown

Gene Name:

mdaB

Uniprot ID:

P0AEY5

Molecular weight:

21891

General function:

Involved in iron ion binding

Specific function:

Part of the anaerobic respiratory chain of trimethylamine-N-oxide reductase torZ. Required for electron transfer to the torZ terminal enzyme

Gene Name:

torY

Uniprot ID:

P52005

Molecular weight:

40286

General function:

Involved in anaerobic electron transport chain

Specific function:

Terminal reductase during anaerobic growth on various sulfoxide and N-oxide compounds. DmsC anchors the dmsAB dimer to the membrane and stabilizes it

Gene Name:

dmsC

Uniprot ID:

P18777

Molecular weight:

30826

General function:

Involved in electron carrier activity

Specific function:

Probable b-type cytochrome

Gene Name:

hyaC

Uniprot ID:

P0AAM1

Molecular weight:

27597

General function:

Involved in catalytic activity

Specific function:

Specific function unknown

Gene Name:

glcE

Uniprot ID:

P52073

Molecular weight:

38361

General function:

Involved in electron carrier activity

Specific function:

Participates in the periplasmic electron-transferring activity of hydrogenase 2 during its catalytic turnover

Gene Name:

hybA

Uniprot ID:

P0AAJ8

Molecular weight:

36003

General function:

Involved in oxidoreductase activity

Specific function:

Reduces trimethylamine-N-oxide (TMAO) into trimethylamine; an anaerobic reaction coupled to energy-yielding reactions

Gene Name:

torA

Uniprot ID:

P33225

Molecular weight:

94455

Reactions

General function:

Involved in metal ion binding

Specific function:

Probable b-type cytochrome

Gene Name:

hybB

Uniprot ID:

P37180

Molecular weight:

43602

General function:

Involved in iron ion binding

Specific function:

Part of the anaerobic respiratory chain of trimethylamine-N-oxide reductase torA. Acts by transferring electrons from the membranous menaquinones to torA. This transfer probably involves an electron transfer pathway from menaquinones to the N-terminal domain of torC, then from the N-terminus to the C-terminus, and finally to torA. TorC apocytochrome negatively autoregulates the torCAD operon probably by inhibiting the torS kinase activity

Gene Name:

torC

Uniprot ID:

P33226

Molecular weight:

43606

General function:

Involved in methyltransferase activity

Specific function:

Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2)

Gene Name:

ubiE

Uniprot ID:

P0A887

Molecular weight:

28073

Reactions