NADPH-dependent 7-cyano-7-deazaguanine reductase (Q46920)

Identification
Name:NADPH-dependent 7-cyano-7-deazaguanine reductase
Synonyms:
  • 7-cyano-7-carbaguanine reductase
  • NADPH-dependent nitrile oxidoreductase
  • PreQ(0) reductase
Gene Name:queF
Enzyme Class:
Biological Properties
General Function:Involved in oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor
Specific Function:Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)
Cellular Location:Cytoplasm (Probable)
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+2.0Thumb
1.07-Aminomethyl-7-carbaguanine + 1.0NADP ↔ 1.07-Cyano-7-carbaguanine + 1.0NADPH + 2.0Hydrogen ion
ReactionCard
SMPDB Reactions:
1.0Thumb+3.0Thumb+2.0NADPH+2.0Thumb → 2.0Thumb+1.0Thumb
1.07-Cyano-7-carbaguanine + 3.0Hydrogen ion + 2.0NADPH + 2.0NADPH → 2.0NADP + 1.07-Aminomethyl-7-carbaguanine
ReactionCard
1.0Thumb+3.0Thumb+2.0NADPH+2.0Thumb → 1.0Queuine+2.0Thumb+1.0Thumb
1.07-Cyano-7-carbaguanine + 3.0Hydrogen ion + 2.0NADPH + 2.0NADPH → 1.0Queuine + 2.0NADP + 1.0Queuine
ReactionCard
Complex Reactions:
3.0Thumb+2.0Thumb+1.0Thumb → 2.0Thumb+1.0Thumb
3.0Hydrogen ion + 2.0NADPH + 1.07-Cyano-7-carbaguanine → 2.0NADP + 1.0Queuine
ReactionCard
1.0Thumb+2.0Thumb → 1.0Thumb+2.0Thumb
1.07-Aminomethyl-7-carbaguanine + 2.0NADP → 1.07-Cyano-7-carbaguanine + 2.0NADPH
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB214567-Aminomethyl-7-carbaguanineMetaboCard
ECMDB202947-Cyano-7-carbaguanineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
ECMDB01495QueuineMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
catalytic activity
oxidoreductase activity
oxidoreductase activity, acting on other nitrogenous compounds as donors
oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
oxidation reduction
queuosine biosynthetic process
RNA metabolic process
tRNA metabolic process
tRNA modification
tRNA processing
Gene Properties
Blattner:b2794
Gene OrientationClockwise
Centisome Percentage:63.01
Left Sequence End2923370
Right Sequence End2924218
Gene Sequence:
>849 bp
ATGAGAAACAAACTCTCTTTCGACTTGCAGTTGAGCGCCAGAAAAGCGGCAATCGCTGAA
CGGATTGCCGCCCATAAAATTGCCCGCAGTAAAGTGTCGGTCTTTTTAATGGCGATGTCC
GCTGGCGTGTTTATGGCGATCGGATTTACTTTTTACCTTTCCGTTATCGCCGATGCCCCG
TCTTCACAGGCATTAACCCATCTGGTGGGCGGCCTTTGCTTTACACTCGGCTTTATTTTG
CTGGCGGTTTGCGGCACCAGCCTGTTCACCTCGTCGGTAATGACGGTGATGGCAAAAAGT
CGGGGCGTTATTAGTTGGCGAACTTGGCTGATTAACGCACTTCTGGTGGCCTGCGGTAAT
CTGGCAGGTATTGCCTGTTTCAGTTTGTTAATCTGGTTTTCCGGGCTGGTGATGAGTGAA
AACGCGATGTGGGGAGTCGCGGTTTTACACTGCGCCGAGGGCAAAATGCATCATACATTT
ACTGAATCTGTCAGCCTCGGCATTATGTGCAATCTGATGGTTTGCCTGGCGCTGTGGATG
AGTTATTGCGGGCGTTCGTTATGCGACAAAATCGTCGCCATGATTTTGCCCATCACCCTG
TTTGTCGCCAGTGGCTTTGAGCACTGTATCGCCAATTTGTTTGTGATTCCGTTCGCCATT
GCCATTCGCCATTTCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAAT
TTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGT
AATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTATCGGGCTATTTATTTACGTCAG
GAACCCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:282
Protein Molecular Weight:32587
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>NADPH-dependent 7-cyano-7-deazaguanine reductase
MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGLKADNLPFHGTDIWTLY
ELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQTRFNNWDEVRQTLERDLS
TCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNYEFTTDYLENATCGEKVVEET
LVSHLLKSNCLITHQPDWGSLQIQYRGRQIDREKLLRYLVSFRHHNEFHEQCVERIFNDL
LRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPSTTRLVRQ
References
External Links:
ResourceLink
Uniprot ID:Q46920
Uniprot Name:QUEF_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1805553
Ecogene ID:EG13173
Ecocyc:EG13173
ColiBase:b2794
Kegg Gene:b2794
EchoBASE ID:EB2965
CCDB:QUEF_ECOLI
BacMap:16130701
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Van Lanen, S. G., Reader, J. S., Swairjo, M. A., de Crecy-Lagard, V., Lee, B., Iwata-Reuyl, D. (2005). "From cyclohydrolase to oxidoreductase: discovery of nitrile reductase activity in a common fold." Proc Natl Acad Sci U S A 102:4264-4269. Pubmed: 15767583