Identification
Name:Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase
Synonyms:
  • Hydroxymethylpyrimidine kinase
  • HMP kinase
  • Hydroxymethylpyrimidine phosphate kinase
  • HMP-P kinase
  • HMP-phosphate kinase
  • HMPP kinase
Gene Name:thiD
Enzyme Class:
Biological Properties
General Function:Involved in phosphomethylpyrimidine kinase activity
Specific Function:Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
SMPDB Reactions:
1.04-amino-2-methyl-5-phosphomethylpyrimidine+1.0Thumb1.0Thumb+1.0Thumb
1.04-amino-2-methyl-5-phosphomethylpyrimidine + 1.0Adenosine triphosphate → 1.0ADP + 1.02-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
Complex Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB200462-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphateMetaboCard
ECMDB200844-Amino-2-methyl-5-phosphomethylpyrimidineMetaboCard
ECMDB200864-Amino-5-hydroxymethyl-2-methylpyrimidineMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
GO Classification:
Function
catalytic activity
kinase activity
phosphomethylpyrimidine kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cellular aromatic compound metabolic process
cellular metabolic process
metabolic process
thiamin and derivative metabolic process
thiamin biosynthetic process
thiamin metabolic process
Gene Properties
Blattner:b2103
Gene OrientationCounterclockwise
Centisome Percentage:47.02
Left Sequence End2181738
Right Sequence End2182538
Gene Sequence:
>801 bp
GTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTA
CCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACC
GAAGAGCGTATTCGGATCCTGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTG
TCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACA
ACGCCAGAGTTAGTACAGTTTGCGACCGCTAACTATCTTGCCGCCGATCAGGTACCCGCT
TTCGTGCTGCCACAACATCCTTCTGGCGTAATGACCCTTTCGGCGGCACATCCAGTGTAT
TGCTGCCCGTTACGTGAACGAGAACCCATTTTGCGTCTGGCGAAGTCGGCACTGGTGACG
GTCTATCCATTACCTGACACCCGCCTGTTGATGGTGGTTAATATACACGCCGTCAACTTC
AGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCAC
CACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAAC
GCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAG
CGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGT
GAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGT
CCCGGCAAGCCTGATAAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:266
Protein Molecular Weight:28633
Protein Theoretical pI:6
PDB File:1JXI
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase
MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQNTRGVQSVYRIEPDFV
AAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNVVLDTVMLAKSGDPLLSPS
AVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLEQGRSLLAMGCGAVLMKGGHL
DDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAALAALRPRHTNWADTVQEAKSWL
SSALAQADTLEVGHGIGPVHHFHAWW
References
External Links:
ResourceLink
Uniprot ID:P76422
Uniprot Name:THID_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674383
PDB ID:1JXI
Ecogene ID:EG14068
Ecocyc:EG14068
ColiBase:b2103
Kegg Gene:b2103
EchoBASE ID:EB3821
CCDB:THID_ECOLI
BacMap:16130041
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Mizote, T., Tsuda, M., Smith, D. D., Nakayama, H., Nakazawa, T. (1999). "Cloning and characterization of the thiD/J gene of Escherichia coli encoding a thiamin-synthesizing bifunctional enzyme, hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase." Microbiology 145 ( Pt 2):495-501. Pubmed: 10075431