Spermidine/putrescine import ATP-binding protein PotA (P69874)

Identification
Name:Spermidine/putrescine import ATP-binding protein PotA
Synonyms:Not Available
Gene Name:potA
Enzyme Class:
Biological Properties
General Function:Involved in nucleotide binding
Specific Function:Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system
Cellular Location:Cell inner membrane; Peripheral membrane protein
SMPDB Pathways:
KEGG Pathways:
Transports:
Transport References:Not Available
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Putrescine → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0Putrescine
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Spermidine → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0Spermidine
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0polyamine(Out) → 1.0Thumb+1.0Thumb+1.0polyamine(In)
1.0Adenosine triphosphate + 1.0Water + 1.0polyamine(Out) → 1.0ADP + 1.0Inorganic phosphate + 1.0polyamine(In)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB01414PutrescineMetaboCard
ECMDB01257SpermidineMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
ATP-binding cassette (ABC) transporter complex
cell part
macromolecular complex
membrane
protein complex
Function
active transmembrane transporter activity
adenyl nucleotide binding
adenyl ribonucleotide binding
amine transmembrane transporter activity
ATP binding
ATPase activity
binding
catalytic activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
nucleoside binding
nucleoside-triphosphatase activity
nucleotide binding
polyamine transmembrane transporter activity
polyamine-transporting ATPase activity
purine nucleoside binding
pyrophosphatase activity
transmembrane transporter activity
transporter activity
Process
amine transport
establishment of localization
polyamine transport
transport
Gene Properties
Blattner:b1126
Gene OrientationCounterclockwise
Centisome Percentage:25.51
Left Sequence End1183681
Right Sequence End1184817
Gene Sequence:
>1137 bp
ATGTTTGATTATGAAACATTGCGCTTCATCTGGTGGCTGCTGATTGGCGTGATCCTGGTG
GTCTTTATGATCTCCGACGGATTTGACATGGGGATCGGCTGTCTGCTGCCGCTGGTGGCG
CGTAATGATGATGAACGCCGGATAGTGATAAACAGCGTTGGTGCACACTGGGAAGGCAAC
CAGGTCTGGTTGATCCTCGCTGGTGGGGCATTATTTGCCGCCTGGCCCAGAGTGTATGCA
GCGGCGTTTTCCGGCTTTTATGTGGCGATGATCCTGGTGCTGTGCTCACTGTTCTTCCGC
CCGCTGGCCTTTGATTATCGCGGAAAAATCGCCGATGCACGCTGGCGTAAAATGTGGGAC
GCCGGTCTGGTCATCGGCAGTCTGGTGCCGCCGGTAGTCTTCGGTATCGCCTTCGGCAAC
TTGTTGCTCGGCGTGCCGTTTGCCTTCACACCGCAATTACGCGTGGAGTATCTCGGCAGC
TTCTGGCAACTGCTGACGCCATTCCCTTTATTGTGCGGATTGCTCAGCCTTGGGATGGTG
ATTTTGCAAGGTGGCGTCTGGTTACAACTGAAAACTGTTGGTGTGATTCATCTGCGTTCA
CAGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTAC
TGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCC
AATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAG
TCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATG
GCGATTTATCGTGGTCGCCCGGGTTGGGGATTTTTGATGGCATCATTGATGCAATTTGGC
GTGATTTTCACGGCAGGCATCACGCTGTTCCCCTTTGTCATGCCGTCAAGCGTGAGTCCG
ATCTCCAGCCTGACGTTGTGGGACAGTACTTCCAGTCAGCTGACGCTGAGCATTATGTTG
GTAATCGTGCTGATATTTTTGCCCATTGTGTTGCTCTACACTCTCTGGAGCTACTACAAA
ATGTGGGGGCGCATGACAACAGAAACTCTCCGCCGTAACGAAAACGAGTTGTACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:378
Protein Molecular Weight:43028
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Spermidine/putrescine import ATP-binding protein PotA
MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGEFLTLLGPSGCGKTTVL
RLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFPHMTVFENVAFGLRMQKTP
AAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIARAVVNKPRLLLLDESLSALDY
KLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRIVVMRDGRIEQDGTPREIYEEPK
NLFVAGFIGEINMFNATVIERLDEQRVRANVEGRECNIYVNFAVEPGQKLHVLLRPEDLR
VEEINDDNHAEGLIGYVRERNYKGMTLESVVELENGKMVMVSEFFNEDDPDFDHSLDQKM
AINWVESWEVVLADEEHK
References
External Links:
ResourceLink
Uniprot ID:P69874
Uniprot Name:POTA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651480
Ecogene ID:EG10749
Ecocyc:EG10749
ColiBase:b1126
Kegg Gene:b1126
EchoBASE ID:EB0742
CCDB:POTA_ECOLI
BacMap:16129089
General Reference:
  • Antognoni, F., Del Duca, S., Kuraishi, A., Kawabe, E., Fukuchi-Shimogori, T., Kashiwagi, K., Igarashi, K. (1999). "Transcriptional inhibition of the operon for the spermidine uptake system by the substrate-binding protein PotD." J Biol Chem 274:1942-1948. Pubmed: 9890949
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Furuchi, T., Kashiwagi, K., Kobayashi, H., Igarashi, K. (1991). "Characteristics of the gene for a spermidine and putrescine transport system that maps at 15 min on the Escherichia coli chromosome." J Biol Chem 266:20928-20933. Pubmed: 1939142
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kashiwagi, K., Endo, H., Kobayashi, H., Takio, K., Igarashi, K. (1995). "Spermidine-preferential uptake system in Escherichia coli. ATP hydrolysis by PotA protein and its association with membrane." J Biol Chem 270:25377-25382. Pubmed: 7592703
  • Kashiwagi, K., Hosokawa, N., Furuchi, T., Kobayashi, H., Sasakawa, C., Yoshikawa, M., Igarashi, K. (1990). "Isolation of polyamine transport-deficient mutants of Escherichia coli and cloning of the genes for polyamine transport proteins." J Biol Chem 265:20893-20897. Pubmed: 2249996
  • Kashiwagi, K., Innami, A., Zenda, R., Tomitori, H., Igarashi, K. (2002). "The ATPase activity and the functional domain of PotA, a component of the sermidine-preferential uptake system in Escherichia coli." J Biol Chem 277:24212-24219. Pubmed: 11976340
  • Kashiwagi, K., Miyamoto, S., Nukui, E., Kobayashi, H., Igarashi, K. (1993). "Functions of potA and potD proteins in spermidine-preferential uptake system in Escherichia coli." J Biol Chem 268:19358-19363. Pubmed: 8366082
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232