Nitrogen regulatory protein (P69829)

Identification
Name:Nitrogen regulatory protein
Synonyms:
  • Enzyme IIA-NTR
  • PTS system EIIA component
  • Phosphotransferase enzyme IIA component
Gene Name:ptsN
Enzyme Class:
Biological Properties
General Function:Involved in transporter activity
Specific Function:Seems to have a role in regulating nitrogen assimilation
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Protein N(pi)-phospho-L-histidine+1.0Sugar ↔ 1.0Protein histidine+1.0Thumb
1.0Protein N(pi)-phospho-L-histidine + 1.0Sugar ↔ 1.0Protein histidine + 1.0Sugar phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
GO Classification:
Function
carbohydrate transmembrane transporter activity
cation transmembrane transporter activity
cation:sugar symporter activity
ion transmembrane transporter activity
protein-N(PI)-phosphohistidine-sugar phosphotransferase activity
solute:cation symporter activity
substrate-specific transmembrane transporter activity
sugar:hydrogen symporter activity
transmembrane transporter activity
transporter activity
Process
carbohydrate transport
establishment of localization
phosphoenolpyruvate-dependent sugar phosphotransferase system
transport
Gene Properties
Blattner:b3204
Gene OrientationClockwise
Centisome Percentage:72.09
Left Sequence End3344600
Right Sequence End3345091
Gene Sequence:
>492 bp
ATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATT
GGCCTTTTTTCCCAGCGTGGCTACAACATTGAAAGCCTGACCGTTGCGCCAACCGACGAT
CCGACATTATCGCGTATGACCATCCAGACCGTGGGCGATGAAAAAGTACTTGAGCAGATC
GAAAAGCAATTACACAAACTGGTCGATGTCTTGCGCGTGAGTGAGTTGGGGCAGGGCGCG
CATGTTGAGCGGGAAATCATGCTGGTGAAAATTCAGGCCAGCGGTTACGGGCGTGACGAA
GTGAAACGTAATACGGAAATATTCCGTGGGCAAATTATCGATGTCACACCCTCGCTTTAT
ACCGTTCAATTAGCAGGCACCAGCGGTAAGCTTGATGCATTTTTAGCATCGATTCGCGAT
GTGGCGAAAATTGTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAA
ATAATGCGTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:163
Protein Molecular Weight:17959
Protein Theoretical pI:6
PDB File:1A6J
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Nitrogen regulatory protein
MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLSLPPQVVFEAILTREKM
GSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQPVDLLFALLVPADQTKTH
LHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTPDEA
References
External Links:
ResourceLink
Uniprot ID:P69829
Uniprot Name:PTSN_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674320
PDB ID:1A6J
Ecogene ID:EG11682
Ecocyc:EG11682
ColiBase:b3204
Kegg Gene:b3204
EchoBASE ID:EB1633
CCDB:PTSN_ECOLI
BacMap:16131094
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bordo, D., van Monfort, R. L., Pijning, T., Kalk, K. H., Reizer, J., Saier, M. H. Jr, Dijkstra, B. W. (1998). "The three-dimensional structure of the nitrogen regulatory protein IIANtr from Escherichia coli." J Mol Biol 279:245-255. Pubmed: 9636714
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Imaishi, H., Gomada, M., Inouye, S., Nakazawa, A. (1993). "Physical map location of the rpoN gene of Escherichia coli." J Bacteriol 175:1550-1551. Pubmed: 8444818
  • Jones, D. H., Franklin, F. C., Thomas, C. M. (1994). "Molecular analysis of the operon which encodes the RNA polymerase sigma factor sigma 54 of Escherichia coli." Microbiology 140 ( Pt 5):1035-1043. Pubmed: 8025669
  • Powell, B. S., Court, D. L., Inada, T., Nakamura, Y., Michotey, V., Cui, X., Reizer, A., Saier, M. H. Jr, Reizer, J. (1995). "Novel proteins of the phosphotransferase system encoded within the rpoN operon of Escherichia coli. Enzyme IIANtr affects growth on organic nitrogen and the conditional lethality of an erats mutant." J Biol Chem 270:4822-4839. Pubmed: 7876255