Identification
Name:Glucose-specific phosphotransferase enzyme IIA component
Synonyms:
  • EIIA-Glc
  • EIII-Glc
  • PTS system glucose-specific EIIA component
Gene Name:crr
Enzyme Class:
Biological Properties
General Function:Involved in sugar:hydrogen symporter activity
Specific Function:The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport
Cellular Location:Cytoplasm
SMPDB Pathways:
  • Amino sugar and nucleotide sugar metabolism I PW000886
  • Amino sugar and nucleotide sugar metabolism II PW000887
  • Amino sugar and nucleotide sugar metabolism III PW000895
  • Galactose metabolism PW000821
  • Starch and sucrose metabolism PW000941
  • Trehalose Degradation I (low osmolarity) PW002097
  • Vitamin B6 1430936196 PW000891
  • colanic acid building blocks biosynthesis PW000951
  • galactose degradation/Leloir Pathway PW000884
  • glycerol metabolism PW000914
  • glycerol metabolism II PW000915
  • glycerol metabolism III (sn-glycero-3-phosphoethanolamine) PW000916
  • glycerol metabolism IV (glycerophosphoglycerol) PW000917
  • glycerol metabolism V (glycerophosphoserine) PW000918
  • glycolysis and pyruvate dehydrogenase PW000785
KEGG Pathways:
KEGG Reactions:
1.0Protein N(pi)-phospho-L-histidine+1.0Thumb1.0Protein histidine+1.0Thumb
1.0Protein N(pi)-phospho-L-histidine + 1.0D-Glucose ↔ 1.0Protein histidine + 1.0Glucose 6-phosphate
ReactionCard
1.0Thumb+1.0Protein N(pi)-phospho-L-histidine1.0Thumb+1.0Protein histidine
1.0Trehalose + 1.0Protein N(pi)-phospho-L-histidine ↔ 1.0Trehalose 6-phosphate + 1.0Protein histidine
ReactionCard
1.0Protein N(pi)-phospho-L-histidine+1.0Thumb1.0Protein histidine+1.0Thumb
1.0Protein N(pi)-phospho-L-histidine + 1.0D-Maltose ↔ 1.0Protein histidine + 1.0Maltose 6'-phosphate
ReactionCard
1.0Protein N(pi)-phospho-L-histidine+1.0Thumb1.0Protein histidine+1.0Thumb
1.0Protein N(pi)-phospho-L-histidine + 1.0Salicin ↔ 1.0Protein histidine + 1.0Salicin 6-phosphate
ReactionCard
1.0Thumb+1.0Protein N(pi)-phospho-L-histidine1.0Thumb+1.0Protein histidine
1.0N-Acetyl-D-muramoate + 1.0Protein N(pi)-phospho-L-histidine ↔ 1.0N-Acetylmuramic acid 6-phosphate + 1.0Protein histidine
ReactionCard
1.0Protein N(pi)-phospho-L-histidine+1.0Sugar1.0Protein histidine+1.0Thumb
1.0Protein N(pi)-phospho-L-histidine + 1.0Sugar ↔ 1.0Protein histidine + 1.0Sugar phosphate
ReactionCard
SMPDB Reactions:
1.0HPr - phosphorylated+1.0MurNAc+1.0MurNAc1.0HPr+1.0Thumb
1.0HPr - phosphorylated + 1.0MurNAc + 1.0MurNAc → 1.0HPr + 1.0MurNAc-6-P
ReactionCard
1.0Erythrose+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Glucosamine+1.0HPr - phosphorylated1.0Thumb+1.0HPr
1.0Glucosamine + 1.0HPr - phosphorylated → 1.0Glucosamine 6-phosphate + 1.0HPr
ReactionCard
1.0Thumb+1.0HPr - phosphorylated1.0Thumb+1.0HPr+1.0Thumb
1.0alpha-D-Glucose + 1.0HPr - phosphorylated → 1.0α-D-glucose 6-phosphate + 1.0HPr + 1.0Mannose 6-phosphate
ReactionCard
1.0Beta-D-Glucose+1.0HPr - phosphorylated+1.0Thumb1.0Thumb+1.0HPr
1.0Beta-D-Glucose + 1.0HPr - phosphorylated + 1.0b-D-Glucose → 1.0beta-D-Glucose 6-phosphate + 1.0HPr
ReactionCard
1.0Thumb+1.0[PTS enzyme I]-Nπ-phospho-L-histidine1.0Thumb+1.0[PTS enzyme I]-L-histidine
1.0D-Glucose + 1.0[PTS enzyme I]-Nπ-phospho-L-histidine → 1.0β-D-glucose 1-phosphate + 1.0[PTS enzyme I]-L-histidine
ReactionCard
1.0Thumb+1.0HPr - phosphorylated1.0Thumb+1.0HPr
1.0α,α-trehalose + 1.0HPr - phosphorylated → 1.0Trehalose 6-phosphate + 1.0HPr
ReactionCard
1.0Thumb+1.0HPr - phosphorylated1.0Thumb+1.0HPr
1.0α,α-trehalose + 1.0HPr - phosphorylated → 1.0alpha,alpha-Trehalose 6-phosphate + 1.0HPr
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
Complex Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0Protein EIIA N(pi)-phospho-L-histidine+1.0protein EIIB1.0protein EIIA+1.0protein EIIB N(pi)-phospho-L-histidine/cysteine
1.0Protein EIIA N(pi)-phospho-L-histidine + 1.0protein EIIB → 1.0protein EIIA + 1.0protein EIIB N(pi)-phospho-L-histidine/cysteine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB04010alpha,alpha-Trehalose 6-phosphateMetaboCard
ECMDB03345alpha-D-GlucoseMetaboCard
ECMDB23064Alpha-D-glucose 6-phosphateMetaboCard
ECMDB20117Arbutin 6-phosphateMetaboCard
ECMDB00516b-D-GlucoseMetaboCard
ECMDB03498beta-D-Glucose 6-phosphateMetaboCard
ECMDB22975CL(17:0cycw7c/16:0/17:0cycw7c/16:1(9Z))MetaboCard
ECMDB22980CL(17:0cycw7c/16:1(9Z)/17:0cycw7c/19:0cycv8c)MetaboCard
ECMDB02649D-Erythrose 4-phosphateMetaboCard
ECMDB00122D-GlucoseMetaboCard
ECMDB00163D-MaltoseMetaboCard
ECMDB24914ErythroseMetaboCard
ECMDB01254Glucosamine 6-phosphateMetaboCard
ECMDB01401Glucose 6-phosphateMetaboCard
ECMDB20171Maltose 6'-phosphateMetaboCard
ECMDB01078Mannose 6-phosphateMetaboCard
ECMDB24235MurNAc-6-PMetaboCard
ECMDB00215N-Acetyl-D-glucosamineMetaboCard
ECMDB01062N-Acetyl-D-Glucosamine 6-PhosphateMetaboCard
ECMDB20176N-Acetyl-D-muramoateMetaboCard
ECMDB20177N-Acetylmuramic acid 6-phosphateMetaboCard
ECMDB00263Phosphoenolpyruvic acidMetaboCard
ECMDB00243Pyruvic acidMetaboCard
ECMDB04151SalicinMetaboCard
ECMDB20191Salicin 6-phosphateMetaboCard
ECMDB00258SucroseMetaboCard
ECMDB20196Sucrose-6-phosphateMetaboCard
ECMDB00975TrehaloseMetaboCard
ECMDB01124Trehalose 6-phosphateMetaboCard
ECMDB24256α,α-trehaloseMetaboCard
ECMDB24239β-D-glucose 1-phosphateMetaboCard
GO Classification:
Component
cell part
membrane
Function
cation transmembrane transporter activity
cation:sugar symporter activity
ion transmembrane transporter activity
solute:cation symporter activity
substrate-specific transmembrane transporter activity
sugar:hydrogen symporter activity
transmembrane transporter activity
transporter activity
Process
carbohydrate transport
establishment of localization
phosphoenolpyruvate-dependent sugar phosphotransferase system
transport
Gene Properties
Blattner:b2417
Gene OrientationClockwise
Centisome Percentage:54.61
Left Sequence End2533856
Right Sequence End2534365
Gene Sequence:
>510 bp
ATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTA
CATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTAT
AAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCT
GATATTCACCAGTGGCAAAATCTGGATGACTATTACAACCAGTACCGCCAACGCGTAGTT
GTTTTGCTTTCTCACCCCGCCAACCCGCGCGATCACACCAATGTTTTGATGCACGTTCAG
GGTTATTTTCGCCCGCATATTGATTCCACAGAACGCCAGCAGCTGGCTGCGCTTATCGAC
AGTTATCGCCGTGGCGAGCAACCACTTCTTGCGCCGCTGATGCGTATCAAACACTATATG
GCGCTTTATCCTGACGCCTGGCTTTCAGGGCAGCGTTATTTCGAACTTTGGCCGCGTGTG
ATTAACTTGCGCCATTCAGGAGTTTTATGA
Protein Properties
Pfam Domain Function:
Protein Residues:169
Protein Molecular Weight:18251
Protein Theoretical pI:4
PDB File:1O2F
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Glucose-specific phosphotransferase enzyme IIA component
MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAEKIVGDGIAIKPTGNKM
VAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGEGFKRIAEEGQRVKVGDTV
IEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVGETPVIRIKK
References
External Links:
ResourceLink
Uniprot ID:P69783
Uniprot Name:PTGA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062300
PDB ID:1O2F
Ecogene ID:EG10165
Ecocyc:EG10165
ColiBase:b2417
Kegg Gene:b2417
EchoBASE ID:EB0163
CCDB:PTGA_ECOLI
BacMap:16130343
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • De Reuse, H., Danchin, A. (1988). "The ptsH, ptsI, and crr genes of the Escherichia coli phosphoenolpyruvate-dependent phosphotransferase system: a complex operon with several modes of transcription." J Bacteriol 170:3827-3837. Pubmed: 2457575
  • Feese, M. D., Comolli, L., Meadow, N. D., Roseman, S., Remington, S. J. (1997). "Structural studies of the Escherichia coli signal transducing protein IIAGlc: implications for target recognition." Biochemistry 36:16087-16096. Pubmed: 9405042
  • Feese, M., Pettigrew, D. W., Meadow, N. D., Roseman, S., Remington, S. J. (1994). "Cation-promoted association of a regulatory and target protein is controlled by protein phosphorylation." Proc Natl Acad Sci U S A 91:3544-3548. Pubmed: 8170944
  • Hall, B. G., Sharp, P. M. (1992). "Molecular population genetics of Escherichia coli: DNA sequence diversity at the celC, crr, and gutB loci of natural isolates." Mol Biol Evol 9:654-665. Pubmed: 1630305
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hurley, J. H., Faber, H. R., Worthylake, D., Meadow, N. D., Roseman, S., Pettigrew, D. W., Remington, S. J. (1993). "Structure of the regulatory complex of Escherichia coli IIIGlc with glycerol kinase." Science 259:673-677. Pubmed: 8430315
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Pelton, J. G., Torchia, D. A., Meadow, N. D., Roseman, S. (1992). "Structural comparison of phosphorylated and unphosphorylated forms of IIIGlc, a signal-transducing protein from Escherichia coli, using three-dimensional NMR techniques." Biochemistry 31:5215-5224. Pubmed: 1606145
  • Pelton, J. G., Torchia, D. A., Meadow, N. D., Wong, C. Y., Roseman, S. (1991). "1H, 15N, and 13C NMR signal assignments of IIIGlc, a signal-transducing protein of Escherichia coli, using three-dimensional triple-resonance techniques." Biochemistry 30:10043-10057. Pubmed: 1911770
  • Pelton, J. G., Torchia, D. A., Meadow, N. D., Wong, C. Y., Roseman, S. (1991). "Secondary structure of the phosphocarrier protein IIIGlc, a signal-transducing protein from Escherichia coli, determined by heteronuclear three-dimensional NMR spectroscopy." Proc Natl Acad Sci U S A 88:3479-3483. Pubmed: 2014267
  • Saffen, D. W., Presper, K. A., Doering, T. L., Roseman, S. (1987). "Sugar transport by the bacterial phosphotransferase system. Molecular cloning and structural analysis of the Escherichia coli ptsH, ptsI, and crr genes." J Biol Chem 262:16241-16253. Pubmed: 2960675
  • Wilkins, M. R., Gasteiger, E., Tonella, L., Ou, K., Tyler, M., Sanchez, J. C., Gooley, A. A., Walsh, B. J., Bairoch, A., Appel, R. D., Williams, K. L., Hochstrasser, D. F. (1998). "Protein identification with N and C-terminal sequence tags in proteome projects." J Mol Biol 278:599-608. Pubmed: 9600841
  • Worthylake, D., Meadow, N. D., Roseman, S., Liao, D. I., Herzberg, O., Remington, S. J. (1991). "Three-dimensional structure of the Escherichia coli phosphocarrier protein IIIglc." Proc Natl Acad Sci U S A 88:10382-10386. Pubmed: 1961703
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837
  • Yang, Y., Zhao, G., Winkler, M. E. (1996). "Identification of the pdxK gene that encodes pyridoxine (vitamin B6) kinase in Escherichia coli K-12." FEMS Microbiol Lett 141:89-95. Pubmed: 8764513