Ammonia channel (P69681)

Identification
Name:Ammonia channel
Synonyms:
  • Ammonia transporter
Gene Name:amtB
Enzyme Class:Not Available
Biological Properties
General Function:Involved in ammonium transmembrane transporter activity
Specific Function:Involved in the uptake of ammonia
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
Metabolites:
ECMDB IDNameView
ECMDB00051AmmoniaMetaboCard
ECMDB21186AmmoniumMetaboCard
GO Classification:
Component
cell part
membrane
Function
ammonium transmembrane transporter activity
cation transmembrane transporter activity
ion transmembrane transporter activity
organic cation transmembrane transporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
establishment of localization
transmembrane transport
transport
Gene Properties
Blattner:b0451
Gene OrientationClockwise
Centisome Percentage:10.18
Left Sequence End472190
Right Sequence End473476
Gene Sequence:
>1287 bp
ATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCAAGCCGTAACC
CAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTG
ACTGAAATTGATGAGATGCTGAAGCTGGATTTTGTCACCCGCAGTGCGAAGATCCTCTCG
GCGTTTATTGGTGATGAAATCCCACAGGAAATCCTGGAAGAGCGCGTGCGCGCGGCGTTT
GCCTTCCCGGCTCCGGTCGCCAATGTTGAAAGCGATGTCGGTTGTCTGGAATTGTTCCAC
GGGCCAACGCTGGCATTTAAAGATTTCGGCGGTCGCTTTATGGCACAAATGCTGACCCAT
ATTGCGGGTGATAAGCCAGTGACCATTCTGACCGCGACCTCCGGTGATACCGGAGCGGCA
GTGGCTCATGCTTTCTACGGTTTACCGAATGTGAAAGTGGTTATCCTCTATCCACGAGGC
AAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTT
GCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAA
GAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTG
GCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAG
CTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAG
TCACTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCA
CGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCG
ATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATC
TGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATG
CGTGAGTTAAAAGAACTGGGCTACACTTCGGAGCCGCACGCTGCCGTAGCTTATCGTGCG
CTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCG
AAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGATCTGCCAAAAGAGCTG
GCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCCGCCGATTTTGCTGCGTTG
CGTAAATTGATGATGAATCATCAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:428
Protein Molecular Weight:44514
Protein Theoretical pI:7
PDB File:1XQF
Signaling Regions:
  • 1-22
Transmembrane Regions:
  • 29-55
  • 60-90
  • 119-141
  • 147-170
  • 185-203
  • 216-242
  • 246-275
  • 280-294
  • 303-328
  • 333-354
  • 370-402
Protein Sequence:
>Ammonia channel
MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLFMTIPGIALFYGGLIRG
KNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINWLMLKNIELTAVMGSIYQY
IHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTLSYIPIAHMVWGGGLLASHGA
LDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPHNLPMVFTGTAILYIGWFGFNAG
SAGTANEIAALAFVNTVVATAAAILGWIFGEWALRGKPSLLGACSGAIAGLVGVTPACGY
IGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPCDVFGVHGVCGIVGCIMTGIFAASSLG
GVGFAEGVTMGHQLLVQLESIAITIVWSGVVAFIGYKLADLTVGLRVPEEQEREGLDVNS
HGENAYNA
References
External Links:
ResourceLink
Uniprot ID:P69681
Uniprot Name:AMTB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321894
PDB ID:1XQF
Ecogene ID:EG11821
Ecocyc:EG11821
ColiBase:b0451
Kegg Gene:b0451
CCDB:AMTB_ECOLI
BacMap:16128436
General Reference:
  • Blakey, D., Leech, A., Thomas, G. H., Coutts, G., Findlay, K., Merrick, M. (2002). "Purification of the Escherichia coli ammonium transporter AmtB reveals a trimeric stoichiometry." Biochem J 364:527-535. Pubmed: 12023896
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Borodovsky, M., Rudd, K. E., Koonin, E. V. (1994). "Intrinsic and extrinsic approaches for detecting genes in a bacterial genome." Nucleic Acids Res 22:4756-4767. Pubmed: 7984428
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Khademi, S., O'Connell, J. 3rd, Remis, J., Robles-Colmenares, Y., Miercke, L. J., Stroud, R. M. (2004). "Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A." Science 305:1587-1594. Pubmed: 15361618
  • Naggert, J., Narasimhan, M. L., DeVeaux, L., Cho, H., Randhawa, Z. I., Cronan, J. E. Jr, Green, B. N., Smith, S. (1991). "Cloning, sequencing, and characterization of Escherichia coli thioesterase II." J Biol Chem 266:11044-11050. Pubmed: 1645722
  • Thomas, G. H., Mullins, J. G., Merrick, M. (2000). "Membrane topology of the Mep/Amt family of ammonium transporters." Mol Microbiol 37:331-344. Pubmed: 10931328
  • Thomas, G., Coutts, G., Merrick, M. (2000). "The glnKamtB operon. A conserved gene pair in prokaryotes." Trends Genet 16:11-14. Pubmed: 10637624
  • van Heeswijk, W. C., Hoving, S., Molenaar, D., Stegeman, B., Kahn, D., Westerhoff, H. V. (1996). "An alternative PII protein in the regulation of glutamine synthetase in Escherichia coli." Mol Microbiol 21:133-146. Pubmed: 8843440
  • Zheng, L., Kostrewa, D., Berneche, S., Winkler, F. K., Li, X. D. (2004). "The mechanism of ammonia transport based on the crystal structure of AmtB of Escherichia coli." Proc Natl Acad Sci U S A 101:17090-17095. Pubmed: 15563598