Probable glycerol-3-phosphate acyltransferase (P60782)

Identification
Name:Probable glycerol-3-phosphate acyltransferase
Synonyms:
  • G3P acyltransferase
  • GPAT
  • Lysophosphatidic acid synthase
  • LPA synthase
Gene Name:plsY
Enzyme Class:
Biological Properties
General Function:Involved in glycerol-3-phosphate O-acyltransferase acti
Specific Function:Catalyzes the transfer of an acyl group from acyl-ACP to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme can also utilize acyl-CoA as fatty acyl donor, but not acyl-PO(4) (Probable)
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Acyl-CoA+1.0Acyl-CoA ↔ 1.01-Acyl-sn-glycerol 3-phosphate+1.0Thumb+1.01-Acyl-sn-glycerol 3-phosphate
1.0Glycerol 3-phosphate + 1.0Acyl-CoA + 1.0Acyl-CoA ↔ 1.01-Acyl-sn-glycerol 3-phosphate + 1.0Coenzyme A + 1.01-Acyl-sn-glycerol 3-phosphate
ReactionCard
1.0Acyl-[acyl-carrier protein]+1.0Thumb ↔ 1.01-Acyl-sn-glycerol 3-phosphate+1.0Acyl-carrier protein
1.0Acyl-[acyl-carrier protein] + 1.0Glycerol 3-phosphate ↔ 1.01-Acyl-sn-glycerol 3-phosphate + 1.0Acyl-carrier protein
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glycerol 3-phosphate + 1.0Hexadecanoyl-phosphate (n-C16:0) → 1.01-hexadecanoyl-sn-glycerol 3-phosphate + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glycerol 3-phosphate + 1.0Octadecanoyl-phosphate (n-C18:0) → 1.01-Octadecanoyl-sn-glycerol 3-phosphate + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glycerol 3-phosphate + 1.0Octadecanoyl-phosphate (n-C18:1) → 1.01-Octadec-11-enoyl-sn-glycerol 3-phosphate + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glycerol 3-phosphate + 1.0Tetradecanoyl-phosphate (n-C14:0) → 1.01-Tetradecanoyl-sn-glycerol 3-phosphate + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Dodecanoly-phosphate (n-C12:0) + 1.0Glycerol 3-phosphate → 1.01-Dodecanoyl-sn-glycerol 3-phosphate + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
1.0Acyl-[acyl-carrier-protein]+1.0Thumb → 1.0[acyl-carrier-protein]+1.01-acyl-sn-glycerol 3-phosphate
1.0Acyl-[acyl-carrier-protein] + 1.0Glycerol 3-phosphate → 1.0[acyl-carrier-protein] + 1.01-acyl-sn-glycerol 3-phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB211271-Dodecanoyl-sn-glycerol 3-phosphateMetaboCard
ECMDB211281-Hexadec-9-enoyl-sn-glycerol 3-phosphateMetaboCard
ECMDB211291-hexadecanoyl-sn-glycerol 3-phosphateMetaboCard
ECMDB211301-Octadec-11-enoyl-sn-glycerol 3-phosphateMetaboCard
ECMDB211311-Octadecanoyl-sn-glycerol 3-phosphateMetaboCard
ECMDB211321-Tetradec-7-enoyl-sn-glycerol 3-phosphateMetaboCard
ECMDB211331-Tetradecanoyl-sn-glycerol 3-phosphateMetaboCard
ECMDB01423Coenzyme AMetaboCard
ECMDB21210Dodecanoly-phosphate (n-C12:0)MetaboCard
ECMDB00126Glycerol 3-phosphateMetaboCard
ECMDB21226Hexadecanoyl-phosphate (n-C16:0)MetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21258Octadecanoyl-phosphate (n-C18:0)MetaboCard
ECMDB21259Octadecanoyl-phosphate (n-C18:1)MetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB21286Tetradecanoyl-phosphate (n-C14:0)MetaboCard
GO Classification:
Component
cell part
membrane
plasma membrane
Gene Properties
Blattner:b3059
Gene OrientationClockwise
Centisome Percentage:69.03
Left Sequence End3202716
Right Sequence End3203333
Gene Sequence:
>618 bp
ATGACAACCCAGTATGGATTTTTTATTGATTCCAGCCGTTGCACCGGTTGCAAAACCTGC
GAGCTGGCCTGTAAAGACTACAAAGATTTGACGCCAGAAGTCAGCTTCCGCCGCATTTAT
GAATATGCTGGCGGCGACTGGCAGGAAGATAACGGTGTCTGGCACCAGAACGTGTTTGCC
TACTATCTGTCGATTTCATGTAACCACTGCGAAGATCCGGCTTGTACTAAAGTCTGCCCG
AGCGGTGCGATGCATAAACGTGAAGATGGTTTTGTTGTGGTCGATGAAGATGTGTGCATT
GGCTGCCGCTACTGCCATATGGCTTGCCCGTATGGCGCACCGCAATATAACGAAACGAAA
GGCCATATGACCAAATGCGATGGTTGTTATGACCGTGTTGCGGAGGGTAAAAAGCCGATC
TGTGTTGAATCTTGTCCGCTGCGGGCGCTGGATTTCGGCCCTATCGACGAGCTGCGTAAA
AAACATGGCGATCTGGCGGCCGTTGCGCCGTTGCCGCGAGCTCACTTTACCAAACCGAAT
ATTGTGATCAAACCCAATGCCAATAGCCGCCCGACCGGGGATACCACTGGCTATCTGGCA
AACCCGAAGGAGGTGTGA
Protein Properties
Pfam Domain Function:
Protein Residues:205
Protein Molecular Weight:22193
Protein Theoretical pI:10
Signaling Regions:
  • None
Transmembrane Regions:
  • 4-24
  • 53-73
  • 81-101
  • 112-132
  • 138-158
Protein Sequence:
>Probable glycerol-3-phosphate acyltransferase
MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGATNVLRIGGKGAAVAVLI
FDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGFKGGKGVATAFGAIAPIGW
DLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKPQFTFPVSMLSCLILLRHHDN
IQRLWRRQETKIWTKFKRKREKDPE
References
External Links:
ResourceLink
Uniprot ID:P60782
Uniprot Name:PLSY_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651422
Ecogene ID:EG11674
Ecocyc:EG11674
ColiBase:b3059
Kegg Gene:b3059
CCDB:PLSY_ECOLI
BacMap:16130955
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Borodovsky, M., Rudd, K. E., Koonin, E. V. (1994). "Intrinsic and extrinsic approaches for detecting genes in a bacterial genome." Nucleic Acids Res 22:4756-4767. Pubmed: 7984428
  • Cain, B. D., Norton, P. J., Eubanks, W., Nick, H. S., Allen, C. M. (1993). "Amplification of the bacA gene confers bacitracin resistance to Escherichia coli." J Bacteriol 175:3784-3789. Pubmed: 8389741
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lu, Y. J., Zhang, Y. M., Grimes, K. D., Qi, J., Lee, R. E., Rock, C. O. (2006). "Acyl-phosphates initiate membrane phospholipid synthesis in Gram-positive pathogens." Mol Cell 23:765-772. Pubmed: 16949372
  • Yoshimura, M., Oshima, T., Ogasawara, N. (2007). "Involvement of the YneS/YgiH and PlsX proteins in phospholipid biosynthesis in both Bacillus subtilis and Escherichia coli." BMC Microbiol 7:69. Pubmed: 17645809