tRNA pseudouridine synthase B (P60340)

Identification
Name:tRNA pseudouridine synthase B
Synonyms:
  • Protein p35
  • tRNA pseudouridine 55 synthase
  • Psi55 synthase
  • tRNA pseudouridylate synthase
  • tRNA-uridine isomerase
Gene Name:truB
Enzyme Class:
Biological Properties
General Function:Translation, ribosomal structure and biogenesis
Specific Function:Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
GO Classification:
Function
binding
catalytic activity
intramolecular transferase activity
isomerase activity
nucleic acid binding
pseudouridine synthase activity
RNA binding
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
pseudouridine synthesis
RNA metabolic process
RNA modification
RNA processing
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>945 bp
ATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAACCACAGCTACGC
ATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACT
CGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTCAGTAACGCTGTTGCACGCCTG
AAGGTGATGTTTAATGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCT
CGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTG
CCTGGTTCAGGTTTTGAACCCGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGC
CCGTTAGACAGCATTCTGACCTCGCAGATTTATAATCACATTGAGCAGATTGCGCCAAAT
ATACATGTTATGTTCAAGTCTTCATTAAATCAGAACACTGAACATCAGCTGCGTTATCAG
GAAACGGAGTTTGTGATTAGTTATGAAGACTTCCATCGTCCTGAATTTACCAGCGTACCA
TTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCG
TTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCG
TCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGC
ATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCG
CGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTA
AAACAAAACAGCAGAACCTGTTATCTCTCCTGGCATGAAGCTGCCGGGCGCGATAAAGGC
CATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:314
Protein Molecular Weight:35087
Protein Theoretical pI:6
PDB File:1R3F
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>tRNA pseudouridine synthase B
MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHTGALDPLATGMLPICLG
EATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVTFSAEQLAAALDTFRGDIE
QIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLFIRHEGNELELEIHCSKGTYI
RTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEHLRELVEQAEQQDIPAAELLDPL
LMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAPLEGLVRVTEGENGKFIGMGEIDDE
GRVAPRRLVVEYPA
References
External Links:
ResourceLink
Uniprot ID:P60340
Uniprot Name:TRUB_ECOLI
GenBank Gene ID:U00096
Genebank Protein ID:87081684
PDB ID:1R3F
CCDB:TRUB_ECOLI
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Gutgsell, N., Englund, N., Niu, L., Kaya, Y., Lane, B. G., Ofengand, J. (2000). "Deletion of the Escherichia coli pseudouridine synthase gene truB blocks formation of pseudouridine 55 in tRNA in vivo, does not affect exponential growth, but confers a strong selective disadvantage in competition with wild-type cells." RNA 6:1870-1881. Pubmed: 11142385
  • Hamilton, C. S., Spedaliere, C. J., Ginter, J. M., Johnston, M. V., Mueller, E. G. (2005). "The roles of the essential Asp-48 and highly conserved His-43 elucidated by the pH dependence of the pseudouridine synthase TruB." Arch Biochem Biophys 433:322-334. Pubmed: 15581587
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hoang, C., Ferre-D'Amare, A. R. (2001). "Cocrystal structure of a tRNA Psi55 pseudouridine synthase: nucleotide flipping by an RNA-modifying enzyme." Cell 107:929-939. Pubmed: 11779468
  • Nurse, K., Wrzesinski, J., Bakin, A., Lane, B. G., Ofengand, J. (1995). "Purification, cloning, and properties of the tRNA psi 55 synthase from Escherichia coli." RNA 1:102-112. Pubmed: 7489483
  • Pan, H., Agarwalla, S., Moustakas, D. T., Finer-Moore, J., Stroud, R. M. (2003). "Structure of tRNA pseudouridine synthase TruB and its RNA complex: RNA recognition through a combination of rigid docking and induced fit." Proc Natl Acad Sci U S A 100:12648-12653. Pubmed: 14566049
  • Ramamurthy, V., Swann, S. L., Spedaliere, C. J., Mueller, E. G. (1999). "Role of cysteine residues in pseudouridine synthases of different families." Biochemistry 38:13106-13111. Pubmed: 10529181
  • Sands, J. F., Regnier, P., Cummings, H. S., Grunberg-Manago, M., Hershey, J. W. (1988). "The existence of two genes between infB and rpsO in the Escherichia coli genome: DNA sequencing and S1 nuclease mapping." Nucleic Acids Res 16:10803-10816. Pubmed: 2849753
  • Spedaliere, C. J., Hamilton, C. S., Mueller, E. G. (2000). "Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues." Biochemistry 39:9459-9465. Pubmed: 10924141