Identification
Name:Glycolate oxidase iron-sulfur subunit
Synonyms:Not Available
Gene Name:glcF
Enzyme Class:Not Available
Biological Properties
General Function:Involved in iron-sulfur cluster binding
Specific Function:Specific function unknown
Cellular Location:Not Available
SMPDB Pathways:
  • glycolate and glyoxylate degradation PW000827
KEGG Pathways:
  • Glyoxylate and dicarboxylate metabolism ec00630
KEGG Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
SMPDB Reactions:
1.0Thumb+1.0an oxidized electron-transfer flavoprotein 1.0Reduced acceptor+1.0Thumb
1.0Glycolic acid + 1.0an oxidized electron-transfer flavoprotein  → 1.0Reduced acceptor + 1.0Glyoxylic acid
ReactionCard
2.0Thumb+2.0an oxidized electron-transfer flavoprotein 2.0Reduced acceptor+2.0Thumb
2.0Glycolic acid + 2.0an oxidized electron-transfer flavoprotein  → 2.0Reduced acceptor + 2.0Glyoxylic acid
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0Thumb+1.0Menaquinone 81.0Thumb+1.0Thumb
1.0Glycolic acid + 1.0Menaquinone 8 → 1.0Glyoxylic acid + 1.0Menaquinol 8
ReactionCard
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB211542-Demethylmenaquinol 8MetaboCard
ECMDB211552-Demethylmenaquinone 8MetaboCard
ECMDB23741GlycolateMetaboCard
ECMDB03035Glycolic acidMetaboCard
ECMDB00119Glyoxylic acidMetaboCard
ECMDB21232Hydrogen peroxideMetaboCard
ECMDB21245Menaquinol 8MetaboCard
ECMDB04124OxygenMetaboCard
ECMDB01060Ubiquinol-8MetaboCard
ECMDB21296Ubiquinone-8MetaboCard
GO Classification:
Function
binding
catalytic activity
electron carrier activity
iron-sulfur cluster binding
metal cluster binding
oxidoreductase activity
Process
metabolic process
oxidation reduction
Gene Properties
Blattner:b4467
Gene OrientationCounterclockwise
Centisome Percentage:67.29
Left Sequence End3122258
Right Sequence End3123481
Gene Sequence:
>1224 bp
ATGAAAAAAATCGCGCTTATTATCGGCAGCATGATCGCGGGCGGTATTATTTCTGCGGCA
GGTTTTACCTGGGTTGCAAAGGCGGAACCGCCTGCAGAAAAAACGTCGACCGCAGAACGT
AAAATCTTATTCTGGTACGACCCAATGTATCCCAATACGCGGTTCGATAAACCAGGTAAA
TCGCCGTTTATGGATATGGATCTGGTGCCGAAATATGCCGATGAAGAGAGTTCTGCGTCT
GGTGTGCGCATTGACCCGACTCAGACGCAGAATCTGGGGGTGAAAACGGCTACCGTCACG
CGCGGACCGCTGACTTTTGCCCAGAGTTTCCCGGCGAATGTCAGTTACAACGAGTATCAG
TATGCCATTGTGCAGGCTCGCGCTGCCGGGTTTATCGACAAGGTGTATCCGCTTACCGTG
GGCGATAAAGTACAAAAGGGCACACCGCTTCTCGACCTGACCATTCCTGACTGGGTGGAA
GCGCAGAGTGAGTATTTACTGCTGCGCGAAACCGGCGGTACGGCGACCCAGACTGAAGGC
ATTCTTGAGCGGCTGCGACTGGCGGGAATGCCGGAGGCGGATATTCGCCGCCTGATCGCC
ACGCAAAAAATCCAGACTCGCTTTACGCTCAAAGCGCCCATTGATGGCGTGATCACCGCG
TTTGATCTGCGCGCGGGAATGAATATCGCCAAAGATAACGTGGTAGCGAAAATTCAGGGT
ATGGACCCGGTGTGGGTCACTGCTGCGATCCCGGAGTCTATCGCCTGGCTGGTGAAAGAT
GCCTCGCAGTTTACCCTCACCGTTCCGGCGCGACCGGATAAAACACTCACCATCCGCAAA
TGGACGCTGCTACCTGGCGTGGATGCCGCGACCCGCACGCTGCAGCTGCGTCTGGAAGTC
GACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTCAACACCGCC
AGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGG
GTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCA
TCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGC
GGCCTGTTCCTGATTGATTCCGAAGCCAATATTTCTGGCGCACTGGAGCGGATGCGCTCT
GAAAGTGCTACCCATGCGCATTGA
Protein Properties
Pfam Domain Function:
Protein Residues:407
Protein Molecular Weight:45110
Protein Theoretical pI:8
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Glycolate oxidase iron-sulfur subunit
MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQVLE
GNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIVEQKVKRPLPERILREGLR
QVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKPRPPLRHKRRVLMLEGCAQPT
LSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNAQEKGLARARNNIDAWWPAIEAG
AEAILQTASGCGAFVKEYGQMLKNDALYADKARQVSELAVDLVELLREEPLEKLAIRGDK
KLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDVPDSHLCCGSAGTYALTHPDLARQLRD
NKMNALESGKPEMIVTANIGCQTHLASAGRTSVRHWIEIVEQALEKE
References
External Links:
ResourceLink
Uniprot ID:P52074
Uniprot Name:GLCF_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062198
Ecogene ID:EG13291
Ecocyc:EG13291
ColiBase:b4467
Kegg Gene:b4467
EchoBASE ID:EB3076
CCDB:GLCF_ECOLI
BacMap:49176294
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Pellicer, M. T., Badia, J., Aguilar, J., Baldoma, L. (1996). "glc locus of Escherichia coli: characterization of genes encoding the subunits of glycolate oxidase and the glc regulator protein." J Bacteriol 178:2051-2059. Pubmed: 8606183
  • Riley, M., Abe, T., Arnaud, M. B., Berlyn, M. K., Blattner, F. R., Chaudhuri, R. R., Glasner, J. D., Horiuchi, T., Keseler, I. M., Kosuge, T., Mori, H., Perna, N. T., Plunkett, G. 3rd, Rudd, K. E., Serres, M. H., Thomas, G. H., Thomson, N. R., Wishart, D., Wanner, B. L. (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34:1-9. Pubmed: 16397293