Molybdate-binding periplasmic protein (P37329)

Identification
Name:Molybdate-binding periplasmic protein
Synonyms:Not Available
Gene Name:modA
Enzyme Class:Not Available
Biological Properties
General Function:Involved in molybdate transmembrane-transporting ATPase activity
Specific Function:Involved in the transport of molybdenum into the cell. Binds molybdate with high specificity and affinity
Cellular Location:Periplasm
SMPDB Pathways:Not Available
KEGG Pathways:
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Molybdate → 1.0ADP + 1.0Hydrogen ion + 1.0Molybdate + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Sulfate → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0Sulfate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21246MolybdateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB01448SulfateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
outer membrane-bounded periplasmic space
periplasmic space
Function
anion transmembrane transporter activity
inorganic anion transmembrane transporter activity
ion transmembrane transporter activity
molybdate ion transmembrane transporter activity
molybdate transmembrane-transporting ATPase activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
anion transport
establishment of localization
inorganic anion transport
ion transport
molybdate ion transport
transport
Gene Properties
Blattner:b0763
Gene OrientationClockwise
Centisome Percentage:17.12
Left Sequence End794312
Right Sequence End795085
Gene Sequence:
>774 bp
ATGGCTCGTAAATGGTTGAACTTGTTTGCCGGGGCGGCACTCTCTTTCGCTGTTGCTGGC
AATGCACTGGCAGATGAAGGGAAAATCACGGTGTTCGCCGCCGCATCACTGACTAACGCA
ATGCAGGACATTGCTACGCAGTTTAAAAAAGAGAAAGGCGTGGATGTGGTTTCTTCTTTC
GCTTCGTCATCTACTCTCGCCCGTCAGATTGAAGCGGGTGCGCCTGCGGATCTGTTTATT
TCTGCCGATCAGAAATGGATGGATTATGCGGTTGATAAAAAAGCGATCGATACAGCTACG
CGTCAGACACTGCTCGGCAATAGCCTGGTCGTTGTAGCACCGAAAGCCAGCGTGCAGAAA
GATTTCACCATCGACAGCAAAACCAACTGGACTTCACTGCTGAATGGCGGTCGCCTGGCG
GTTGGCGATCCGGAACATGTTCCCGCTGGCATTTATGCAAAAGAAGCACTGCAAAAACTG
GGCGCATGGGATACGCTCTCTCCGAAACTGGCCCCAGCGGAAGATGTTCGTGGGGCGCTG
GCGCTGGTCGAACGTAACGAAGCGCCTCTGGGCATTGTCTACGGTTCTGACGCAGTTGCC
AGCAAAGGGGTAAAAGTGGTTGCCACCTTCCCGGAAGATTCACATAAAAAAGTGGAATAT
CCGGTTGCTGTTGTGGAAGGGCATAACAATGCGACAGTGAAAGCATTTTATGATTATCTG
AAGGGACCGCAGGCAGCGGAAATCTTTAAACGTTACGGATTTACAATCAAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:257
Protein Molecular Weight:27364
Protein Theoretical pI:9
PDB File:1WOD
Signaling Regions:
  • 1-24
Transmembrane Regions:
  • None
Protein Sequence:
>Molybdate-binding periplasmic protein
MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDIATQFKKEKGVDVVSSF
ASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQTLLGNSLVVVAPKASVQK
DFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKLGAWDTLSPKLAPAEDVRGAL
ALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKVEYPVAVVEGHNNATVKAFYDYL
KGPQAAEIFKRYGFTIK
References
External Links:
ResourceLink
Uniprot ID:P37329
Uniprot Name:MODA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651348
PDB ID:1WOD
Ecogene ID:EG12427
Ecocyc:EG12427
ColiBase:b0763
Kegg Gene:b0763
EchoBASE ID:EB2325
CCDB:MODA_ECOLI
BacMap:16128731
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hu, Y., Rech, S., Gunsalus, R. P., Rees, D. C. (1997). "Crystal structure of the molybdate binding protein ModA." Nat Struct Biol 4:703-707. Pubmed: 9302996
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Rech, S., Deppenmeier, U., Gunsalus, R. P. (1995). "Regulation of the molybdate transport operon, modABCD, of Escherichia coli in response to molybdate availability." J Bacteriol 177:1023-1029. Pubmed: 7860583
  • Rech, S., Wolin, C., Gunsalus, R. P. (1996). "Properties of the periplasmic ModA molybdate-binding protein of Escherichia coli." J Biol Chem 271:2557-2562. Pubmed: 8576221
  • Walkenhorst, H. M., Hemschemeier, S. K., Eichenlaub, R. (1995). "Molecular analysis of the molybdate uptake operon, modABCD, of Escherichia coli and modR, a regulatory gene." Microbiol Res 150:347-361. Pubmed: 8564363