Ribosomal RNA large subunit methyltransferase A (P36999)

Identification
Name:Ribosomal RNA large subunit methyltransferase A
Synonyms:
  • 23S rRNA m1G745 methyltransferase
  • rRNA (guanine-N(1)-)-methyltransferase
Gene Name:rlmA
Enzyme Class:
Biological Properties
General Function:Involved in methyltransferase activity
Specific Function:Specifically methylates the guanosine in position 745 of 23S rRNA
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0rRNA ↔ 1.0Thumb+1.0rRNA containing N1-methylguanine
1.0S-Adenosylmethionine + 1.0rRNA ↔ 1.0S-Adenosylhomocysteine + 1.0rRNA containing N1-methylguanine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00939S-AdenosylhomocysteineMetaboCard
ECMDB01185S-AdenosylmethionineMetaboCard
GO Classification:
Function
catalytic activity
methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
Process
metabolic process
Gene Properties
Blattner:b1822
Gene OrientationCounterclockwise
Centisome Percentage:41.04
Left Sequence End1904275
Right Sequence End1905084
Gene Sequence:
>810 bp
ATGACGAAGCATACTCTTGAGCAACTGGCGGCGGATTTACGCCGCGCCGCAGAGCAGGGC
GAAGCGATTGCACCGCTGCGCGATCTGATTGGTATCGATAACGCTGAAGCGGCTTACGCC
ATTCAGCACATAAATGTGCAACATGACGTTGCGCAGGGGCGTCGCGTGGTAGGGCGTAAA
GTGGGCCTGACACATCCGAAAGTGCAACAACAACTGGGCGTTGATCAACCGGATTTTGGG
ACGTTATTTGCCGACATGTGTTATGGCGATAACGAAATCATTCCTTTTTCCCGTGTTCTG
CAACCCCGCATTGAAGCGGAGATCGCACTGGTGTTGAACCGCGATTTGCCCGCAACCGAT
ATCACCTTCGACGAATTGTATAACGCCATTGAATGGGTACTTCCGGCGCTGGAAGTGGTG
GGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCC
TGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACGTCCGGCGGGGTTAGACCTGAAAAAC
TGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGC
CTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAA
CCGCTGCGCACCGGAGATATCATTCTTACCGGGGCATTAGGTCCGATGGTGGCGGTGAAT
GCGGGCGATCGTTTTGAAGCCCATATTGAAGGCATAGGTTCAGTTGCTGCGACATTTTCA
AGCGCAGCCCCAAAAGGAAGTCTGTCATGA
Protein Properties
Pfam Domain Function:
Protein Residues:269
Protein Molecular Weight:30419
Protein Theoretical pI:7
PDB File:1P91
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Ribosomal RNA large subunit methyltransferase A
MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKRSRDPGDSAEMMQARRA
FLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHAFADALPEITTFGLDVSKV
AIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCKAEELARVVKPGGWVITATPG
PRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCYPMRLRGDEAVALLQMTPFAWRA
KPEVWQTLAAKEVFDCQTDFNIHLWQRSY
References
External Links:
ResourceLink
Uniprot ID:P36999
Uniprot Name:RLMA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674492
PDB ID:1P91
Ecogene ID:EG12207
Ecocyc:EG12207
ColiBase:b1822
Kegg Gene:b1822
EchoBASE ID:EB2122
CCDB:RLMA_ECOLI
BacMap:16129776
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Das, K., Acton, T., Chiang, Y., Shih, L., Arnold, E., Montelione, G. T. (2004). "Crystal structure of RlmAI: implications for understanding the 23S rRNA G745/G748-methylation at the macrolide antibiotic-binding site." Proc Natl Acad Sci U S A 101:4041-4046. Pubmed: 14999102
  • Gustafsson, C., Persson, B. C. (1998). "Identification of the rrmA gene encoding the 23S rRNA m1G745 methyltransferase in Escherichia coli and characterization of an m1G745-deficient mutant." J Bacteriol 180:359-365. Pubmed: 9440525
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Yamanaka, K., Mitani, T., Ogura, T., Niki, H., Hiraga, S. (1994). "Cloning, sequencing, and characterization of multicopy suppressors of a mukB mutation in Escherichia coli." Mol Microbiol 13:301-312. Pubmed: 7984109