Identification
Name:Malate:quinone oxidoreductase
Synonyms:
  • MQO
  • Malate dehydrogenase [quinone]
Gene Name:mqo
Enzyme Class:
Biological Properties
General Function:Involved in malate dehydrogenase (quinone) activity
Specific Function:(S)-malate + a quinone = oxaloacetate + reduced quinone
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
Thumb+ThumbThumb+Thumb
Thumb+ThumbThumb+Thumb
SMPDB Reactions:
L-Malic acid+Thumb+ThumbThumb+Thumb
EcoCyc Reactions:
Thumb+a quinoneThumb+a quinol
L-Malic acid + a quinone → Oxalacetic acid + a quinol
ReactionCard
Complex Reactions:
Thumb+ThumbThumb+Thumb
Thumb+Menaquinone 8Thumb+Thumb
Thumb+a quinoneThumb+reduced quinone
L-Malic acid + a quinone → Oxalacetic acid + reduced quinone
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01248FADMetaboCard
ECMDB01197FADH2MetaboCard
ECMDB21232Hydrogen peroxideMetaboCard
ECMDB02434HydroquinoneMetaboCard
ECMDB00156L-Malic acidMetaboCard
ECMDB21245Menaquinol 8MetaboCard
ECMDB00223Oxalacetic acidMetaboCard
ECMDB04124OxygenMetaboCard
ECMDB23060QuinoneMetaboCard
ECMDB01060Ubiquinol-8MetaboCard
ECMDB21296Ubiquinone-8MetaboCard
GO Classification:
Function
catalytic activity
malate dehydrogenase (quinone) activity
malate dehydrogenase activity
oxidoreductase activity
oxidoreductase activity, acting on CH-OH group of donors
Process
acetyl-CoA catabolic process
acetyl-CoA metabolic process
cellular metabolic process
coenzyme metabolic process
cofactor metabolic process
metabolic process
oxidation reduction
tricarboxylic acid cycle
Gene Properties
Blattner:b2210
Gene OrientationCounterclockwise
Centisome Percentage:49.64
Left Sequence End2303130
Right Sequence End2304776
Gene Sequence:
>1647 bp
ATGAAAAAAGTGACTGCCATGCTCTTCTCGATGGCCGTGGGGCTTAATGCCGTTTCGATG
GCGGCAAAAGCGAAAGCGTCCGAGGAGCAGGAAACTGATGTACTGTTGATTGGCGGCGGC
ATTATGAGCGCCACGTTGGGGACCTATTTACGCGAGCTGGAGCCTGAATGGTCGATGACC
ATGGTGGAGCGCCTGGAGGGTGTCGCGCAGGAGAGTTCGAACGGCTGGAATAACGCCGGA
ACCGGGCATTCTGCACTGATGGAACTGAACTACACCCCGCAAAACGCCGATGGCAGCATC
AGTATTGAAAAAGCAGTCGCCATTAACGAAGCATTTCAGATTTCCCGCCAGTTCTGGGCG
CACCAGGTTGAGCGCGGCGTGCTGCGTACTCCGCGTTCATTTATCAATACCGTTCCGCAT
ATGAGCTTTGTCTGGGGCGAGGATAACGTCAATTTCCTGCGCGCCCGTTACGCCGCGTTG
CAACAAAGCTCGCTGTTTCGCGGTATGCGTTACTCTGAAGATCACGCGCAGATCAAAGAG
TGGGCACCGTTAGTGATGGAAGGGCGCGATCCGCAACAGAAAGTGGCAGCCACGCGTACG
GAAATTGGTACCGATGTGAACTACGGCGAGATCACCCGCCAGTTAATTGCTTCCTTGCAG
AAGAAATCTAACTTCTCGCTGCAACTCAGCAGCGAAGTCCGCGCCCTAAAGCGTAATGAC
GATAACACCTGGACCGTTACCGTTGCCGATCTGAAAAATGGCACTGCACAGAACATTCGT
GCCAAATTTGTCTTTATCGGCGCGGGCGGTGCGGCGCTGAAGCTGTTACAGGAATCGGGG
ATTCCGGAAGCGAAAGACTACGCCGGTTTCCCGGTGGGCGGACAGTTCCTTGTTTCGGAA
AACCCGGACGTGGTTAATCACCATCTGGCGAAGGTTTACGGTAAAGCATCCGTTGGCGCA
CCACCGATGTCGGTTCCGCATATCGATACCCGCGTTCTGGACGGTAAACGCGTAGTGCTG
TTTGGGCCATTTGCCACCTTCTCAACCAAATTCCTCAAAAACGGTTCATTGTGGGATCTA
ATGAGTTCCACCACCACCTCTAACGTGATGCCGATGATGCACGTCGGGCTGGATAATTTC
GATCTGGTGAAATATCTGGTGAGTCAGGTGATGTTGAGTGAAGAGGATCGTTTTGAAGCG
TTGAAAGAGTACTATCCGCAGGCGAAAAAAGAGGACTGGCGTTTGTGGCAAGCGGGGCAG
CGCGTGCAGATTATCAAGCGTGATGCCGAGAAAGGTGGCGTACTGCGTCTGGGTACTGAA
GTCGTCAGTGACCAGCAAGGAACCATTGCCGCGCTCCTGGGGGCATCGCCAGGGGCGTCA
ACCGCCGCGCCGATTATGTTGAATCTGCTGGAAAAAGTATTTGGCGATCGTGTTTCCAGC
CCGCAATGGCAGGCTACGTTGAAAGCGATCGTTCCGTCTTATGGACGCAAGCTGAACGGT
GATGTAGCGGCAACAGAACGCGAGTTGCAGTACACCAGCGAAGTGCTGGGGTTGAACTAC
GACAAGCCGCAGGCAGCAGATAGTACGCCGAAACCGCAGTTGAAACCGCAACCCGTTCAA
AAAGAAGTGGCGGATATTGCGTTGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:548
Protein Molecular Weight:60229
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Malate:quinone oxidoreductase
MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSATLGTYLRELEPEWSMT
MVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISIEKAVAINEAFQISRQFWA
HQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAALQQSSLFRGMRYSEDHAQIKE
WAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIASLQKKSNFSLQLSSEVRALKRND
DNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSE
NPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDL
MSSTTTSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQ
RVQIIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSS
PQWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLNYDKPQAADSTPKPQLKPQPVQ
KEVADIAL
References
External Links:
ResourceLink
Uniprot ID:P33940
Uniprot Name:MQO_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1736851
Ecogene ID:EG12069
Ecocyc:EG12069
ColiBase:b2210
Kegg Gene:b2210
EchoBASE ID:EB1996
CCDB:MQO_ECOLI
BacMap:16130147
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Kather, B., Stingl, K., van der Rest, M. E., Altendorf, K., Molenaar, D. (2000). "Another unusual type of citric acid cycle enzyme in Helicobacter pylori: the malate:quinone oxidoreductase." J Bacteriol 182:3204-3209. Pubmed: 10809701