L-carnitine/gamma-butyrobetaine antiporter (P31553)

Identification
Name:L-carnitine/gamma-butyrobetaine antiporter
Synonyms:Not Available
Gene Name:caiT
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transporter activity
Specific Function:Catalyzes the exchange of L-carnitine for gamma- butyrobetaine and related betaines
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Metabolites:
ECMDB IDNameView
ECMDB00062CarnitineMetaboCard
ECMDB21336gamma-ButyrobetaineMetaboCard
GO Classification:
Component
cell part
membrane
Function
transporter activity
Process
establishment of localization
transport
Gene Properties
Blattner:b0040
Gene OrientationCounterclockwise
Centisome Percentage:0.87
Left Sequence End40417
Right Sequence End41931
Gene Sequence:
>1515 bp
ATGAAGAATGAAAAGAGAAAAACGGGAATAGAACCGAAGGTTTTCTTTCCGCCGTTAATA
ATCGTCGGCATACTTTGTTGGCTTACAGTCAGAGATCTGGATGCAGCGAATGTCGTTATT
AATGCTGTATTCAGTTACGTCACCAATGTATGGGGATGGGCATTTGAATGGTATATGGTG
GTGATGCTTTTCGGTTGGTTCTGGCTGGTGTTTGGCCCGTATGCCAAAAAGCGTTTAGGT
AACGAACCGCCAGAATTTAGCACCGCCAGTTGGATCTTTATGATGTTCGCCTCCTGTACG
TCTGCTGCCGTACTGTTCTGGGGATCGATTGAGATCTACTACTACATCTCCACCCCGCCG
TTTGGCTTAGAACCGAACTCGACAGGGGCGAAAGAGTTGGGGCTGGCTTACAGCTTGTTC
CACTGGGGACCTCTGCCGTGGGCCACTTACAGCTTCCTTTCAGTCGCCTTCGCTTACTTC
TTCTTTGTCCGCAAAATGGAAGTGATTCGCCCCAGCTCGACACTGGTGCCGCTGGTAGGT
GAAAAACACGCCAAAGGGTTGTTCGGCACTATCGTCGACAACTTCTATCTCGTCGCCTTG
ATCTTCGCGATGGGTACCAGTCTGGGCCTTGCCACGCCGCTGGTGACCGAGTGTATGCAA
TGGTTGTTTGGCATTCCGCATACCCTGCAACTGGACGCTATCATCATTACCTGCTGGATT
ATCCTCAACGCCATTTGCGTCGCTTGCGGTCTGCAAAAAGGGGTACGTATCGCCAGTGAC
GTGCGTAGTTACCTGAGCTTCCTGATGCTGGGTTGGGTGTTCATTGTCAGCGGTGCCAGC
TTCATCATGAACTACTTCACCGATTCGGTGGGGATGTTGCTGATGTATCTGCCGCGCATG
TTGTTCTATACCGATCCCATCGCTAAAGGCGGCTTCCCGCAGGGCTGGACCGTGTTCTAC
TGGGCATGGTGGGTGATTTATGCTATCCAGATGAGTATCTTCCTCGCCCGCATCTCCCGT
GGTCGTACTGTGCGTGAACTGTGCTTCGGCATGGTGCTGGGGCTGACAGCGTCAACCTGG
ATCCTGTGGACTGTACTCGGTAGTAACACTCTGCTGTTGATAGATAAAAACATCATCAAC
ATTCCAAATCTGATCGAACAGTACGGTGTGGCGCGCGCCATCATTGAAACCTGGGCCGCT
CTGCCACTCAGCACCGCCACCATGTGGGGCTTCTTCATCCTCTGCTTTATTGCCACCGTT
ACGCTGGTTAACGCCTGCTCTTATACCCTGGCGATGTCCACTTGCCGCGAAGTACGCGAT
GGTGAAGAACCACCTCTGCTGGTGCGTATCGGTTGGTCAATTCTGGTTGGCATTATCGGT
ATTGTTCTGCTGGCGCTCGGCGGCCTGAAACCGATTCAAACCGCCATTATCGCCGGAGGA
TGCCCGCTGTTCTTCGTCAACATTATGGTGACGCTCTCCTTTATTAAAGACGCGAAACAG
AACTGGAAAGATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:504
Protein Molecular Weight:56587
Protein Theoretical pI:8
Signaling Regions:
  • None
Transmembrane Regions:
  • 10-30
  • 51-71
  • 92-112
  • 143-163
  • 195-215
  • 231-251
  • 263-283
  • 316-336
  • 347-367
  • 398-418
  • 446-466
  • 475-495
Protein Sequence:
>L-carnitine/gamma-butyrobetaine antiporter
MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVFSYVTNVWGWAFEWYMV
VMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSAAVLFWGSIEIYYYISTPP
FGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYFFFVRKMEVIRPSSTLVPLVG
EKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTECMQWLFGIPHTLQLDAIIITCWI
ILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIVSGASFIMNYFTDSVGMLLMYLPRM
LFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIFLARISRGRTVRELCFGMVLGLTASTW
ILWTVLGSNTLLLIDKNIINIPNLIEQYGVARAIIETWAALPLSTATMWGFFILCFIATV
TLVNACSYTLAMSTCREVRDGEEPPLLVRIGWSILVGIIGIVLLALGGLKPIQTAIIAGG
CPLFFVNIMVTLSFIKDAKQNWKD
References
External Links:
ResourceLink
Uniprot ID:P31553
Uniprot Name:CAIT_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674295
Ecogene ID:EG11561
Ecocyc:EG11561
ColiBase:b0040
Kegg Gene:b0040
EchoBASE ID:EB1522
CCDB:CAIT_ECOLI
BacMap:16128034
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Eichler, K., Bourgis, F., Buchet, A., Kleber, H. P., Mandrand-Berthelot, M. A. (1994). "Molecular characterization of the cai operon necessary for carnitine metabolism in Escherichia coli." Mol Microbiol 13:775-786. Pubmed: 7815937
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Jung, H., Buchholz, M., Clausen, J., Nietschke, M., Revermann, A., Schmid, R., Jung, K. (2002). "CaiT of Escherichia coli, a new transporter catalyzing L-carnitine/gamma -butyrobetaine exchange." J Biol Chem 277:39251-39258. Pubmed: 12163501
  • Lenski, R. E., Winkworth, C. L., Riley, M. A. (2003). "Rates of DNA sequence evolution in experimental populations of Escherichia coli during 20,000 generations." J Mol Evol 56:498-508. Pubmed: 12664169
  • Yura, T., Mori, H., Nagai, H., Nagata, T., Ishihama, A., Fujita, N., Isono, K., Mizobuchi, K., Nakata, A. (1992). "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Nucleic Acids Res 20:3305-3308. Pubmed: 1630901