Identification
Name:Probable crotonobetaine/carnitine-CoA ligase
Synonyms:Not Available
Gene Name:caiC
Enzyme Class:Not Available
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Could catalyze the transfer of CoA to carnitine, generating the initial carnitinyl-CoA needed for the CaiB reaction cycle
Cellular Location:Not Available
SMPDB Pathways:
  • L-carnitine degradation I PW002037
  • Operon: 16S ribosomal RNA modification & chaperone PW001877
  • Operon: probable carnitine operon PW001875
KEGG Pathways:Not Available
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB00045Adenosine monophosphateMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB00062CarnitineMetaboCard
ECMDB01423Coenzyme AMetaboCard
ECMDB21321CrotonobetaineMetaboCard
ECMDB20539Crotonobetainyl-CoAMetaboCard
ECMDB20541D-Carnitinyl-CoAMetaboCard
ECMDB23900L-CarnitineMetaboCard
ECMDB20578L-Carnitinyl-CoAMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Function
catalytic activity
Process
metabolic process
Gene Properties
Blattner:b0037
Gene OrientationCounterclockwise
Centisome Percentage:0.78
Left Sequence End36271
Right Sequence End37824
Gene Sequence:
>1569 bp
ATGGATAGAGGTGCAATGGATATCATTGGCGGACAACATCTACGTCAAATGTGGGACGAT
CTTGCGGACGTTTACGGTCATAAAACGGCGCTGATTTGTGAATCCAGCGGCGGAGTCGTT
AACCGGTATAGTTATCTTGAGTTAAATCAGGAGATTAACCGCACGGCAAACCTGTTTTAT
ACGCTGGGGATTCGCAAAGGCGACAAGGTTGCACTACATCTCGACAACTGCCCGGAATTT
ATCTTTTGCTGGTTCGGGCTGGCAAAAATTGGCGCGATTATGGTGCCGATTAACGCCCGC
CTGTTGTGCGAGGAAAGCGCGTGGATCCTGCAAAATAGCCAGGCGTGCCTGCTGGTGACC
AGTGCGCAATTCTATCCTATGTATCAACAGATTCAGCAGGAAGATGCCACTCAATTGCGG
CACATTTGCCTGACAGATGTGGCACTTCCCGCTGATGATGGCGTGAGTTCGTTTACTCAA
CTGAAAAATCAACAACCTGCCACCTTGTGCTATGCACCGCCGCTATCGACTGACGATACG
GCGGAAATTCTCTTCACCTCCGGCACCACCTCCCGACCGAAAGGTGTGGTGATTACCCAT
TACAACCTGCGCTTCGCTGGATATTACTCCGCCTGGCAGTGTGCACTGCGTGACGATGAC
GTCTACCTGACGGTAATGCCTGCGTTTCATATCGATTGCCAGTGTACTGCGGCGATGGCG
GCGTTTTCTGCCGGGGCCACCTTTGTGCTGGTCGAGAAATACAGCGCCCGCGCCTTCTGG
GGACAGGTACAGAAGTACCGCGCCACCGTTACCGAATGTATTCCGATGATGATCCGTACG
TTGATGGTACAGCCGCCTTCAGCGAACGATCAGCAACACCGCCTGCGGGAAGTGATGTTT
TATCTCAACTTGTCGGAGCAGGAAAAAGATGCGTTTTGTGAACGCTTCGGCGTTCGCTTG
CTGACGTCTTATGGGATGACGGAAACCATTGTGGGCATTATCGGCGATCGTCCTGGCGAT
AAACGACGCTGGCCGTCGATTGGTCGGGTGGGGTTTTGCTACGAAGCGGAGATCCGCGAC
GATCACAATCGCCCGCTCCCGGCTGGTGAGATCGGTGAAATCTGCATTAAAGGCATACCT
GGGAAAACCATCTTCAAAGAGTACTTTCTCAACCCACAAGCCACTGCGAAAGTGCTGGAA
GCCGATGGCTGGCTGCATACCGGCGATACCGGATACCGCGACGAAGAGGACTTTTTTTAT
TTCGTCGATCGCCGCTGCAATATGATTAAACGTGGCGGCGAGAATGTCTCCTGCGTGGAG
CTGGAAAATATTATCGCCGCGCACCCGAAAATTCAGGACATCGTGGTTGTGGGTATTAAA
GATTCGATTCGCGATGAAGCCATCAAAGCATTTGTGGTGCTGAATGAAGGTGAAACATTG
AGCGAAGAGGAATTTTTCCGCTTCTGCGAACAAAATATGGCGAAATTTAAAGTGCCCTCT
TATCTGGAGATCAGAAAAGATCTGCCACGTAATTGCTCGGGGAAAATAATTAGAAAGAAT
CTGAAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:517
Protein Molecular Weight:58559
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Probable crotonobetaine/carnitine-CoA ligase
MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELNQEINRTANLFYTLGIR
KGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLCEESAWILQNSQACLLVTSAQFY
PMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQPATLCYAPPLSTDDTAEILF
TSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYLTVMPAFHIDCQCTAAMAAFSAG
ATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTLMVQPPSANDQQHRLREVMFYLNLS
EQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPGDKRRWPSIGRVGFCYEAEIRDDHNRP
LPAGEIGEICIKGIPGKTIFKEYFLNPQATAKVLEADGWLHTGDTGYRDEEDFFYFVDRR
CNMIKRGGENVSCVELENIIAAHPKIQDIVVVGIKDSIRDEAIKAFVVLNEGETLSEEEF
FRFCEQNMAKFKVPSYLEIRKDLPRNCSGKIIRKNLK
References
External Links:
ResourceLink
Uniprot ID:P31552
Uniprot Name:CAIC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321919
Ecogene ID:EG11558
Ecocyc:EG11558
ColiBase:b0037
Kegg Gene:b0037
EchoBASE ID:EB1519
CCDB:CAIC_ECOLI
BacMap:221800780
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Eichler, K., Bourgis, F., Buchet, A., Kleber, H. P., Mandrand-Berthelot, M. A. (1994). "Molecular characterization of the cai operon necessary for carnitine metabolism in Escherichia coli." Mol Microbiol 13:775-786. Pubmed: 7815937
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Heider, J. (2001). "A new family of CoA-transferases." FEBS Lett 509:345-349. Pubmed: 11749953
  • Lenski, R. E., Winkworth, C. L., Riley, M. A. (2003). "Rates of DNA sequence evolution in experimental populations of Escherichia coli during 20,000 generations." J Mol Evol 56:498-508. Pubmed: 12664169
  • Stenmark, P., Gurmu, D., Nordlund, P. (2004). "Crystal structure of CaiB, a type-III CoA transferase in carnitine metabolism." Biochemistry 43:13996-14003. Pubmed: 15518548
  • Yura, T., Mori, H., Nagai, H., Nagata, T., Ishihama, A., Fujita, N., Isono, K., Mizobuchi, K., Nakata, A. (1992). "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Nucleic Acids Res 20:3305-3308. Pubmed: 1630901