DNA polymerase III subunit chi (P28905)

Identification
Name:DNA polymerase III subunit chi
Synonyms:Not Available
Gene Name:holC
Enzyme Class:
Biological Properties
General Function:Involved in DNA binding
Specific Function:DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0dATP + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0dGTP + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0dCTP + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0Thymidine 5'-triphosphate + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Deoxynucleoside triphosphate+1.0DNA ↔ 1.0Thumb
1.0Deoxynucleoside triphosphate + 1.0DNA ↔ 1.0Pyrophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01532dATPMetaboCard
ECMDB00998dCTPMetaboCard
ECMDB01440dGTPMetaboCard
ECMDB04142PyrophosphateMetaboCard
ECMDB01342Thymidine 5'-triphosphateMetaboCard
GO Classification:
Function
binding
catalytic activity
DNA binding
DNA polymerase activity
DNA-directed DNA polymerase activity
nucleic acid binding
nucleotidyltransferase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cellular macromolecule metabolic process
DNA metabolic process
DNA replication
macromolecule metabolic process
metabolic process
Gene Properties
Blattner:b4259
Gene OrientationCounterclockwise
Centisome Percentage:96.60
Left Sequence End4481860
Right Sequence End4482303
Gene Sequence:
>444 bp
ATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGAT
GCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGATGTCAGCAGGCGGAAGAACAGCTCAAA
ATGCTGATTGATTATCAGAATGAATATCGCAATAACCTCAACAGCGATATGAGTGCCGGG
ATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGACGCTGGAAAAAGCCATT
ACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGT
TGGCGAGAAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACG
GCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAGATGGATGAGTTCGCCCAGCGC
GCCGCCATGAGGAAACCTGAATGA
Protein Properties
Pfam Domain Function:
Protein Residues:147
Protein Molecular Weight:16633
Protein Theoretical pI:6
PDB File:1EM8
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>DNA polymerase III subunit chi
MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACEDEKQAYRLDEALWARP
AESFVPHNLAGEGPRGGAPVEIAWPQKRSSSRRDILISLRTSFADFATAFTEVVDFVPYE
DSLKQLARERYKAYRVAGFNLNTATWK
References
External Links:
ResourceLink
Uniprot ID:P28905
Uniprot Name:HOLC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1736608
PDB ID:1EM8
Ecogene ID:EG11413
Ecocyc:EG11413
ColiBase:b4259
Kegg Gene:b4259
EchoBASE ID:EB1385
CCDB:HOLC_ECOLI
BacMap:16132081
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Carter, J. R., Franden, M. A., Lippincott, J. A., McHenry, C. S. (1993). "Identification, molecular cloning and characterization of the gene encoding the chi subunit of DNA polymerase III holoenzyme of Escherichia coli." Mol Gen Genet 241:399-408. Pubmed: 8246893
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • O'Donnell, M. (1992). "Accessory protein function in the DNA polymerase III holoenzyme from E. coli." Bioessays 14:105-111. Pubmed: 1575709
  • Stirling, C. J., Colloms, S. D., Collins, J. F., Szatmari, G., Sherratt, D. J. (1989). "xerB, an Escherichia coli gene required for plasmid ColE1 site-specific recombination, is identical to pepA, encoding aminopeptidase A, a protein with substantial similarity to bovine lens leucine aminopeptidase." EMBO J 8:1623-1627. Pubmed: 2670557
  • Xiao, H., Crombie, R., Dong, Z., Onrust, R., O'Donnell, M. (1993). "DNA polymerase III accessory proteins. III. holC and holD encoding chi and psi." J Biol Chem 268:11773-11778. Pubmed: 8389364
  • Xiao, H., Dong, Z., O'Donnell, M. (1993). "DNA polymerase III accessory proteins. IV. Characterization of chi and psi." J Biol Chem 268:11779-11784. Pubmed: 8505305