Identification
Name:O-antigen ligase
Synonyms:Not Available
Gene Name:rfaL
Enzyme Class:Not Available
Biological Properties
General Function:Cell wall/membrane/envelope biogenesis
Specific Function:Adds the O-antigen on the glucose group of LPS
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
KEGG Pathways:
  • Lipopolysaccharide biosynthesis ec00540
EcoCyc Reactions:
1.0Thumb+1.0colanic acid1.0MLPS
1.0Lipid A-core + 1.0colanic acid → 1.0MLPS
ReactionCard
Complex Reactions:
1.0core oligosaccharide lipid A+1.0(enterobacterial common antigen)x4 undecaprenyl-diphosphate1.0(enterobacterial common antigen)x4 core oligosaccharide lipid A+1.0Thumb+1.0Thumb
1.0core oligosaccharide lipid A + 1.0(enterobacterial common antigen)x4 undecaprenyl-diphosphate → 1.0(enterobacterial common antigen)x4 core oligosaccharide lipid A + 1.0Hydrogen ion + 1.0Undecaprenyl diphosphate
ReactionCard
1.0core oligosaccharide lipid A+1.0(O16 antigen)x4 undecaprenyl diphosphate1.0Thumb+1.0(O16 antigen)x4 core oligosaccharide lipid A+1.0Thumb
1.0core oligosaccharide lipid A + 1.0(O16 antigen)x4 undecaprenyl diphosphate → 1.0Hydrogen ion + 1.0(O16 antigen)x4 core oligosaccharide lipid A + 1.0Undecaprenyl diphosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB20535Lipid A-coreMetaboCard
ECMDB01469Undecaprenyl diphosphateMetaboCard
GO Classification:Not Available
Gene Properties
Blattner:b3622
Gene OrientationClockwise
Centisome Percentage:81.79
Left Sequence End3794971
Right Sequence End3796230
Gene Sequence:
>1260 bp
GTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTA
TTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTATGTGGACCGGC
GGCACTCTCGGAACCGGTCTTAGCTATCATGATTTCTTCCTCGCAGTTCTCATCGGTAAT
CTTCTCCTCGGTATTTACACTTCATTTCTCGGTTACATTGGCGCAAAAACCGGCCTGACC
ACTCATCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTA
CTGGGCGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTG
GGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACC
GTCACCGTCTTTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCT
ATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATGGGCGGCCTGGAC
GCATTAAAAGCGGTCGTTCCCGCACAACCGTTAGATTTCAATGTCGCGCTGGCGCTGGTT
GTGGGGTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAAT
GCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTT
ATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATT
GCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAAC
GATAACGCACTCTATGCGTCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAA
ACCCTTTCGGTAATCAACGGTATTATCGGTACGGTCTGCGCATTATGGCTGTATAACAAT
TTTGTCGGCTGGTTGACCTTCCTTTCGGCAGCTATTCCTCCAGTGGGTGGCGTGATCATC
GCCGACTATCTGATGAACCGTCGCCGCTATGAGCACTTTGCGACCACGCGTATGATGAGT
GTCAATTGGGTGGCGATTCTGGCGGTCGCCTTGGGGATTGCTGCAGGCCACTGGTTACCG
GGAATTGTTCCGGTCAACGCGGTATTAGGTGGCGCGCTGAGCTATCTGATCCTTAACCCG
ATTTTGAATCGTAAAACGACAGCAGCAATGACGCATGTGGAGGCTAACAGTGTCGAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:419
Protein Molecular Weight:46877
Protein Theoretical pI:10
Signaling Regions:
  • None
Transmembrane Regions:
  • 18-38
  • 40-60
  • 72-92
  • 106-126
  • 136-156
  • 169-189
  • 194-214
  • 216-236
  • 242-262
  • 347-367
  • 370-390
  • 391-411
Protein Sequence:
>O-antigen ligase
MLTSFKLHSLKPYTLKSSMILEIITYILCFFSMIIAFVDNTFSIKIYNITAIVCLLSLIL
RGRQENYNIKNLILPLSIFLIGLLDLIWYSAFKVDNSPFRATYHSYLNTAKIFIFGSFIV
FLTLTSQLKSKKESVLYTLYSLSFLIAGYAMYINSIHENDRISFGVGTATGAAYSTMLIG
IVSGVAILYTKKNHPFLFLLNSCAVLYVLALTQTRATLLLFPIICVAALIAYYNKSPKKF
TSSIVLLIAILASIVIIFNKPIQNRYNEALNDLNSYTNANSVTSLGARLAMYEIGLNIFI
KSPFSFRSAESRAESMNLLVAEHNRLRGALEFSNVHLHNEIIEAGSLKGLMGIFSTLFLY
FSLFYIAYKKRALGLLILTLGIVGIGLSDVIIWARSIPIIIISAIVLLLVINNRNNTIN
References
External Links:
ResourceLink
Uniprot ID:P27243
Uniprot Name:RFAL_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674478
Ecogene ID:EG11424
Ecocyc:EG11424
ColiBase:b3622
Kegg Gene:b3622
EchoBASE ID:EB1394
CCDB:RFAL_ECOLI
BacMap:16131493
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Klena, J. D., Pradel, E., Schnaitman, C. A. (1992). "Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium." J Bacteriol 174:4746-4752. Pubmed: 1624462
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620