Identification
Name:Lipopolysaccharide core heptose(I) kinase rfaP
Synonyms:Not Available
Gene Name:rfaP
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transferase activity, transferring phosphorus-containing groups
Specific Function:Catalyzes the phosphorylation of heptose(I) of the outer membrane lipopolysaccharide core
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Lipopolysaccharide biosynthesis ec00540
SMPDB Reactions:
1.0Thumb+1.0Thumb1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0Thumb
EcoCyc Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
Complex Reactions:
1.0Thumb+1.0heptosyl-heptosyl-kdo2-lipidA1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0heptosyl-heptosyl-kdo2-lipidA → 1.0ADP + 1.0Hydrogen ion + 1.0Phospho-heptosyl-heptosyl-kdo2-lipidA
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB24220glucosyl-(heptosyl)2-Kdo2-lipid AMetaboCard
ECMDB24221glucosyl-(heptosyl)2-Kdo2-lipid A-phosphateMetaboCard
ECMDB20528Glucosyl-heptosyl2-KDO2-lipid AMetaboCard
ECMDB20529Glucosyl-heptosyl2-KDO2-lipid A-phosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21350Phospho-heptosyl-heptosyl-kdo2-lipidAMetaboCard
GO Classification:
Component
cell part
membrane
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
nucleoside binding
phosphotransferase activity, alcohol group as acceptor
purine nucleoside binding
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
lipid biosynthetic process
lipid metabolic process
lipopolysaccharide biosynthetic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b3630
Gene OrientationCounterclockwise
Centisome Percentage:81.97
Left Sequence End3803176
Right Sequence End3803973
Gene Sequence:
>798 bp
ATGCAGGAATACACGAATCATTCCGATACCACTTTTGCACTGCGTAATATCTCCTTTCGT
GTGCCCGGGCGCACGCTTTTGCATCCGCTGTCGTTAACCTTTCCTGCCGGGAAAGTGACC
GGTCTGATTGGTCACAACGGTTCTGGTAAATCCACTCTGCTCAAAATGCTTGGCCGTCAT
CAGCCGCCGTCGGAAGGGGAGATTCTTCTTGATGCCCAACCGCTGGAAAGCTGGAGCAGC
AAAGCGTTTGCCCGCAAAGTGGCTTATTTGCCGCAGCAGCTTCCTCCGGCAGAAGGGATG
ACCGTGCGTGAACTGGTGGCGATTGGTCGTTACCCGTGGCATGGCGCGCTGGGGCGCTTT
GGGGCGGCAGATCGCGAAAAAGTCGAGGAAGCTATCTCGCTGGTTGGCTTAAAACCGCTG
GCGCATCGGCTGGTCGATAGTCTCTCTGGCGGCGAACGTCAGCGGGCGTGGATCGCCATG
CTGGTGGCGCAGGATAGCCGTTGTCTGTTGCTCGACGAACCGACCTCGGCGCTGGATATC
GCCCACCAGGTTGATGTGCTGTCGCTGGTGCACCGTTTAAGTCAGGAGCGTGGCCTGACG
GTCATTGCCGTGTTGCACGATATCAATATGGCGGCACGCTACTGTGATTATCTGGTCGCC
CTGCGCGGCGGTGAAATGATTGCTCAGGGAACGCCTGCGGAAATTATGCGCGGCGAAACC
CTCGAAATGATTTATGGCATCCCGATGGGTATTTTGCCGCATCCGGCGGGTGCTGCACCT
GTGAGTTTTGTTTATTGA
Protein Properties
Pfam Domain Function:
Protein Residues:265
Protein Molecular Weight:30872
Protein Theoretical pI:11
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Lipopolysaccharide core heptose(I) kinase rfaP
MVELKEPLATLWRGKDAFAEVKKLNGEVFRELETRRTLRFELSGKSYFLKWHKGTTLKEI
IKNLLSLRMPVLGADREWHAIHRLSDVGVDTMKGIGFGEKGLNPLTRASFIITEDLTPTI
SLEDYCADWAVNPPDIRVKRMLIARVATMVRKMHTAGINHRDCYICHFLLHLPFTGREDE
LKISVIDLHRAQIRAKVPRRWRDKDLIGLYFSSMNIGLTQRDIWRFMKVYFGMPLRKILS
LEQNLLNMASVKAERIKERTQRKGL
References
External Links:
ResourceLink
Uniprot ID:P25741
Uniprot Name:RFAP_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674357
Ecogene ID:EG11340
Ecocyc:EG11340
ColiBase:b3630
Kegg Gene:b3630
EchoBASE ID:EB1316
CCDB:RFAP_ECOLI
BacMap:16131501
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Parker, C. T., Pradel, E., Schnaitman, C. A. (1992). "Identification and sequences of the lipopolysaccharide core biosynthetic genes rfaQ, rfaP, and rfaG of Escherichia coli K-12." J Bacteriol 174:930-934. Pubmed: 1732225
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620