Identification
Name:Anaerobic dimethyl sulfoxide reductase chain A
Synonyms:
  • DMSO reductase
Gene Name:dmsA
Enzyme Class:
Biological Properties
General Function:Involved in dimethyl sulfoxide reductase activity
Specific Function:Terminal reductase during anaerobic growth on various sulfoxide and N-oxide compounds. Allows E.coli to grow anaerobically on Me(2)SO as respiratory oxidant
Cellular Location:Cell membrane; Peripheral membrane protein; Cytoplasmic side
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb
SMPDB Reactions:
1.0Thumb+1.0Thumb+2.0Thumb+2.0Electron1.0menaquinone-8+1.0Thumb+1.0Thumb+2.0Thumb
1.0Menaquinol 8 + 1.0Dimethyl sulfoxide + 2.0Hydrogen ion + 2.0Electron → 1.0menaquinone-8 + 1.0Dimethyl sulfide + 1.0Water + 2.0Hydrogen ion
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Menaquinone 8+1.0Thumb
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Menaquinone 8
1.0Dimethyl sulfoxide + 1.0Menaquinol 8 → 1.0Dimethyl sulfide + 1.0Water + 1.0Menaquinone 8
ReactionCard
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB211542-Demethylmenaquinol 8MetaboCard
ECMDB211552-Demethylmenaquinone 8MetaboCard
ECMDB21207Dimethyl sulfideMetaboCard
ECMDB21208Dimethyl sulfoxideMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21551Menaquinol 6MetaboCard
ECMDB21245Menaquinol 8MetaboCard
ECMDB04102MenaquinoneMetaboCard
ECMDB00906TrimethylamineMetaboCard
ECMDB04161Trimethylamine N-OxideMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
4 iron, 4 sulfur cluster binding
binding
catalytic activity
cation binding
dimethyl sulfoxide reductase activity
electron carrier activity
ion binding
iron-sulfur cluster binding
metal cluster binding
metal ion binding
molybdenum ion binding
oxidoreductase activity
oxidoreductase activity, acting on a sulfur group of donors
transition metal ion binding
Process
metabolic process
oxidation reduction
Gene Properties
Blattner:b0894
Gene OrientationClockwise
Centisome Percentage:20.26
Left Sequence End940182
Right Sequence End942626
Gene Sequence:
>2445 bp
ATGAAAACGAAAATCCCTGATGCGGTATTGGCTGCTGAGGTGAGTCGCCGTGGTTTGGTA
AAAACGACAGCGATCGGCGGCCTGGCAATGGCCAGCAGCGCATTAACATTACCTTTTAGT
CGGATTGCGCACGCTGTCGATAGCGCCATTCCAACAAAATCAGACGAAAAGGTTATCTGG
AGCGCCTGTACAGTTAACTGTGGTAGTCGCTGCCCGCTACGTATGCACGTCGTGGACGGT
GAAATCAAATATGTCGAAACGGACAATACCGGCGATGACAATTACGACGGCCTGCACCAG
GTTCGCGCCTGCCTGCGTGGGCGTTCCATGCGTCGCCGTGTCTACAATCCGGACCGCCTG
AAATATCCGATGAAACGAGTCGGGGCGCGCGGTGAAGGCAAATTCGAGCGCATTAGCTGG
GAAGAAGCCTACGACATCATCGCGACCAATATGCAGCGCCTGATCAAAGAGTACGGCAAC
GAGTCTATCTATCTGAACTATGGCACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGG
CCGCCGGGAAATACCCTGGTCGCGCGGCTGATGAACTGCTGCGGCGGCTATCTGAACCAT
TACGGCGACTACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGG
GCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAAT
AACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGC
CAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGG
CGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTG
GCGTACGTCATGATCACTGAAAACCTGGTGGATCAGGCATTCCTCGATAAATATTGCGTT
GGCTACGATGAGAAAACCCTGCCAGCCAGTGCGCCGAAAAATGGCCACTATAAAGCTTAT
ATTCTGGGTGAAGGGCCAGATGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACT
GGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAATCGGTAGTACCAAACCGGCG
TTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCT
ATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGAGGCAACAGCGGCGCG
CGTGAAGGTTCATACAGCTTACCGTTTGTCCGTATGCCGACCTTGGAAAACCCGATCCAG
ACCAGCATTTCGATGTTTATGTGGACCGATGCCATTGAACGTGGCCCGGAAATGACGGCG
CTGCGTGATGGTGTACGCGGGAAAGATAAGCTGGATGTGCCGATCAAAATGATCTGGAAC
TATGCCGGTAACTGCCTGATTAACCAGCATTCTGAAATCAACCGTACCCATGAAATCCTT
CAGGATGATAAGAAGTGCGAGCTGATTGTGGTTATCGACTGCCACATGACCTCATCGGCG
AAATATGCTGACATCCTGCTGCCTGACTGCACCGCTTCCGAACAGATGGACTTTGCGCTG
GATGCATCCTGCGGGAATATGTCTTACGTGATTTTCAACGATCAGGTGATTAAACCGCGC
TTTGAATGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAG
CAACAGTTTACTGAAGGCCGTACCCAGGAAGAGTGGATGCGGCATCTGTATGCCCAGTCG
CGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCCGCAAGCAGGGGATCTTTAAA
AAGCGCGACCCACAAGGGCATCACGTTGCTTATAAAGCCTTCCGTGAAGATCCGCAGGCA
AACCCACTGACTACGCCATCGGGCAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATT
GCCGCTACCTGGGAATTGCCTGAAGGCGATGTGATCGATCCACTGCCGATCTACACGCCG
GGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATCCGCTGCAGCTTACAGGTTTC
CACTATAAATCTCGCGTTCACTCAACTTACGGCAACGTTGATGTGCTGAAAGCGGCTTGC
CGTCAGGAAATGTGGATCAACCCGCTTGATGCCCAAAAACGCGGTATCCACAACGGCGAT
AAAGTCAGGATCTTTAACGATCGTGGTGAGGTTCATATTGAGGCGAAAGTGACGCCACGA
ATGATGCCGGGTGTGGTCGCACTGGGTGAAGGTGCCTGGTATGACCCGGATGCAAAACGT
GTCGATAAGGGTGGTTGTATTAACGTACTGACCACTCAACGTCCGTCTCCTCTCGCTAAG
GGGAATCCGTCACATACAAACCTTGTTCAGGTTGAAAAGGTGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:814
Protein Molecular Weight:90398
Protein Theoretical pI:7
Signaling Regions:
  • 1-45
Transmembrane Regions:
  • None
Protein Sequence:
>Anaerobic dimethyl sulfoxide reductase chain A
MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAHAVDSAIPTKSDEKVIW
SACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVRACLRGRSMRRRVYNPDRL
KYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGNESIYLNYGTGTLGGTMTRSW
PPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYGGWADGNSPSDIENSKLVVLFGN
NPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTDTGAGREDEWIPIRPGTDAALVNGL
AYVMITENLVDQAFLDKYCVGYDEKTLPASAPKNGHYKAYILGEGPDGVAKTPEWASQIT
GVPADKIIKLAREIGSTKPAFISQGWGPQRHANGEIATRAISMLAILTGNVGINGGNSGA
REGSYSLPFVRMPTLENPIQTSISMFMWTDAIERGPEMTALRDGVRGKDKLDVPIKMIWN
YAGNCLINQHSEINRTHEILQDDKKCELIVVIDCHMTSSAKYADILLPDCTASEQMDFAL
DASCGNMSYVIFNDQVIKPRFECKTIYEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQS
REAIPELPTFEEFRKQGIFKKRDPQGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADI
AATWELPEGDVIDPLPIYTPGFESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAAC
RQEMWINPLDAQKRGIHNGDKVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKR
VDKGGCINVLTTQRPSPLAKGNPSHTNLVQVEKV
References
External Links:
ResourceLink
Uniprot ID:P18775
Uniprot Name:DMSA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674786
Ecogene ID:EG10232
Ecocyc:EG10232
ColiBase:b0894
Kegg Gene:b0894
EchoBASE ID:EB0228
CCDB:DMSA_ECOLI
BacMap:90111183
General Reference:
  • Bilous, P. T., Cole, S. T., Anderson, W. F., Weiner, J. H. (1988). "Nucleotide sequence of the dmsABC operon encoding the anaerobic dimethylsulphoxide reductase of Escherichia coli." Mol Microbiol 2:785-795. Pubmed: 3062312
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Sambasivarao, D., Scraba, D. G., Trieber, C., Weiner, J. H. (1990). "Organization of dimethyl sulfoxide reductase in the plasma membrane of Escherichia coli." J Bacteriol 172:5938-5948. Pubmed: 2170332
  • Sambasivarao, D., Turner, R. J., Simala-Grant, J. L., Shaw, G., Hu, J., Weiner, J. H. (2000). "Multiple roles for the twin arginine leader sequence of dimethyl sulfoxide reductase of Escherichia coli." J Biol Chem 275:22526-22531. Pubmed: 10801884
  • Trieber, C. A., Rothery, R. A., Weiner, J. H. (1994). "Multiple pathways of electron transfer in dimethyl sulfoxide reductase of Escherichia coli." J Biol Chem 269:7103-7109. Pubmed: 8125918
  • Tullman-Ercek, D., DeLisa, M. P., Kawarasaki, Y., Iranpour, P., Ribnicky, B., Palmer, T., Georgiou, G. (2007). "Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides." J Biol Chem 282:8309-8316. Pubmed: 17218314