Identification
Name:Enterochelin esterase
Synonyms:
  • Ferric enterobactin esterase
Gene Name:fes
Enzyme Class:Not Available
Biological Properties
General Function:Involved in iron ion binding
Specific Function:Upon internalization, ferric enterobactin is processed via an exquisitely specific pathway that is dependent on FES activity, making iron available for metabolic use
Cellular Location:Cytoplasm
SMPDB Pathways:
  • Biosynthesis of siderophore group nonribosomal peptides PW000760
KEGG Pathways:
  • Biosynthesis of siderophore group nonribosomal peptides ec01053
EcoCyc Reactions:
1.0Thumb+3.0Thumb3.0Thumb+3.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb
1.0(2,3-dihydroxybenzoylserine)3+1.0Thumb ? 1.0Thumb
1.0(2,3-dihydroxybenzoylserine)3 + 1.0Water ? 1.02,3-Dihydroxybenzoylserine
ReactionCard
Complex Reactions:
1.0Thumb+3.0Thumb3.0Thumb+1.0Thumb+3.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB211382,3-DihydroxybenzoylserineMetaboCard
ECMDB21212EnterochelinMetaboCard
ECMDB21395Fe3+MetaboCard
ECMDB21331ferric 2,3-dihydroxybenzoylserineMetaboCard
ECMDB21372Ferric enterobactinMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
binding
catalytic activity
cation binding
enterochelin esterase activity
hydrolase activity
hydrolase activity, acting on ester bonds
ion binding
iron ion binding
metal ion binding
transition metal ion binding
Process
cation transport
establishment of localization
ion transport
iron ion transport
metal ion transport
transition metal ion transport
transport
Gene Properties
Blattner:b0585
Gene OrientationClockwise
Centisome Percentage:13.19
Left Sequence End612038
Right Sequence End613162
Gene Sequence:
>1125 bp
ATGTTTGAGGTCACTTTCTGGTGGCGTGATCCCCAAGGTTCTGAAGAATACTCGACGATA
AAGCGCGTATGGGTCTACATCACTGGTGTGACCGATCACCATCAGAACAGCCAGCCCCAG
TCGATGCAGCGAATTGCAGGCACTAACGTCTGGCAGTGGACGACACAACTCAATGCCAAC
TGGCGCGGCAGCTACTGCTTTATTCCCACCGAACGCGATGACATTTTTTCTGTACCATCC
CCCGATCGCCTCGAATTGCGCGAAGGCTGGCGAAAACTATTACCCCAGGCGATAGCCGAT
CCGCTGAACCTACAAAGCTGGAAAGGCGGGCGAGGGCACGCTGTTTCTGCACTCGAAATG
CCGCAAGCGCCTCTGCAACCGGGATGGGATTGTCCGCAAGCGCCAGAAATACCTGCCAAA
GAAATTATCTGGAAAAGTGAACGGTTGAAAAAGTCACGGCGTGTATGGATTTTTACCACC
GGCGATGCAACAGCAGAAGAACGCCCGCTGGCAGTTTTGCTCGATGGCGAATTTTGGGCG
CAAAGTATGCCCGTCTGGCCAGTGCTGACTTCGCTGACCCATCGTCAGCAACTTCCTCCC
GCCGTGTATGTGTTGATCGACGCTATCGACACCACGCACCGCGCCCACGAACTGCCGTGT
AATGCGGATTTCTGGCTCGCAGTACAGCAAGAGTTATTACCCCTGGTGAAAGCTATTGCC
CCTTTTAGCGATCGTGCCGATCGCACCGTGGTTGCCGGGCAGAGTTTTGGTGGGCTTTCC
GCGCTGTATGCCGGACTGCACTGGCCTGAACGCTTTGGCTGTGTATTAAGCCAGTCAGGA
TCGTACTGGTGGCCGCATCGGGGCGGGCAGCAAGAGGGCGTGTTACTTGAAAAGCTAAAA
GCTGGTGAAGTTAGCGCCGAAGGTCTGCGCATTGTGCTGGAAGCGGGTATTCGCGAGCCG
ATGATCATGCGGGCCAATCAGGCGCTGTATGCGCAATTACACCCCATAAAAGAATCCATT
TTCTGGCGTCAGGTTGACGGCGGACATGATGCGCTTTGTTGGCGCGGTGGCTTGATGCAG
GGGCTAATCGACCTCTGGCAACCACTTTTCCATGACAGGAGTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:374
Protein Molecular Weight:42569
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Enterochelin esterase
MFEVTFWWRDPQGSEEYSTIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNAN
WRGSYCFIPTERDDIFSVPSPDRLELREGWRKLLPQAIADPLNLQSWKGGRGHAVSALEM
PQAPLQPGWDCPQAPEIPAKEIIWKSERLKKSRRVWIFTTGDATAEERPLAVLLDGEFWA
QSMPVWPVLTSLTHRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIA
PFSDRADRTVVAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLK
AGEVSAEGLRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQ
GLIDLWQPLFHDRS
References
External Links:
ResourceLink
Uniprot ID:P13039
Uniprot Name:FES_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674705
Ecogene ID:EG10299
Ecocyc:EG10299
ColiBase:b0585
Kegg Gene:b0585
EchoBASE ID:EB0295
CCDB:FES_ECOLI
BacMap:16128568
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Pettis, G. S., Brickman, T. J., McIntosh, M. A. (1988). "Transcriptional mapping and nucleotide sequence of the Escherichia coli fepA-fes enterobactin region. Identification of a unique iron-regulated bidirectional promoter." J Biol Chem 263:18857-18863. Pubmed: 2974033